scholarly journals Hepatocyte Nuclear Factor 4α (HNF4α) in Coordination With Retinoic Acid Receptors Increasesall-trans-Retinoic Acid-Dependent CYP26A1 Gene Expression in HepG2 Human Hepatocytes

2014 ◽  
Vol 115 (10) ◽  
pp. 1740-1751 ◽  
Author(s):  
Reza Zolfaghari ◽  
A. Catharine Ross
Keyword(s):  
Gene Expression ◽  
Retinoic Acid ◽  
Human Hepatocytes ◽  
Retinoic Acid Receptors ◽  
Nuclear Factor ◽  
Hepatocyte Nuclear Factor ◽  
Trans Retinoic Acid ◽  
Hepatocyte Nuclear Factor 4Α

Activation of p21CIP1/WAF1 gene expression and inhibition of cell proliferation by overexpression of hepatocyte nuclear factor-4α

2005 ◽  
Vol 302 (1) ◽  
pp. 11-21 ◽  
Author(s):  
Hideki Chiba ◽  
Taisei Itoh ◽  
Seiro Satohisa ◽  
Naoyuki Sakai ◽  
Hiroko Noguchi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Nuclear Factor ◽  
Hepatocyte Nuclear Factor ◽  
Hepatocyte Nuclear Factor 4Α

scholarly journals Role of hepatocyte nuclear factor 4α in control of blood coagulation factor gene expression

2006 ◽  
Vol 84 (4) ◽  
pp. 345-345
Author(s):  
Yusuke Inoue ◽  
L. Luanne Peters ◽  
Sun Hee Yim ◽  
Junko Inoue ◽  
Frank J. Gonzalez
Keyword(s):  
Gene Expression ◽  
Blood Coagulation ◽  
Coagulation Factor ◽  
Nuclear Factor ◽  
Hepatocyte Nuclear Factor ◽  
Factor Gene ◽  
Hepatocyte Nuclear Factor 4Α

scholarly journals Hepatocyte-specific deletion of hepatocyte nuclear factor-4α in adult mice results in increased hepatocyte proliferation

2013 ◽  
Vol 304 (1) ◽  
pp. G26-G37 ◽  
Author(s):  
Chad Walesky ◽  
Sumedha Gunewardena ◽  
Ernest F. Terwilliger ◽  
Genea Edwards ◽  
Prachi Borude ◽  
...  
Keyword(s):  
Gene Expression ◽  
Target Genes ◽  
Viral Vector ◽  
Hepatocyte Proliferation ◽  
Nuclear Factor ◽  
Hepatocyte Nuclear Factor ◽  
Hepatocyte Differentiation ◽  
Hepatic Differentiation ◽  
Hepatocyte Nuclear Factor 4Α ◽  
Specific Deletion

Hepatocyte nuclear factor-4α (HNF4α) is known as the master regulator of hepatocyte differentiation. Recent studies indicate that HNF4α may inhibit hepatocyte proliferation via mechanisms that have yet to be identified. Using a HNF4α knockdown mouse model based on delivery of inducible Cre recombinase via an adeno-associated virus 8 viral vector, we investigated the role of HNF4α in the regulation of hepatocyte proliferation. Hepatocyte-specific deletion of HNF4α resulted in increased hepatocyte proliferation. Global gene expression analysis showed that a majority of the downregulated genes were previously known HNF4α target genes involved in hepatic differentiation. Interestingly, ≥500 upregulated genes were associated with cell proliferation and cancer. Furthermore, we identified potential negative target genes of HNF4α, many of which are involved in the stimulation of proliferation. Using chromatin immunoprecipitation analysis, we confirmed binding of HNF4α at three of these genes. Furthermore, overexpression of HNF4α in mouse hepatocellular carcinoma cells resulted in a decrease in promitogenic gene expression and cell cycle arrest. Taken together, these data indicate that, apart from its role in hepatocyte differentiation, HNF4α actively inhibits hepatocyte proliferation by repression of specific promitogenic genes.

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