Nanotopography Drives Stem Cell Fate Toward Osteoblast Differentiation Through α1β1 Integrin Signaling Pathway

2014 ◽  
Vol 115 (3) ◽  
pp. 540-548 ◽  
Author(s):  
A.L. Rosa ◽  
R.B. Kato ◽  
L.M.S. Castro Raucci ◽  
L.N. Teixeira ◽  
F.S. de Oliveira ◽  
...  
Keyword(s):  
Stem Cell ◽  
Signaling Pathway ◽  
Cell Fate ◽  
Osteoblast Differentiation ◽  
Integrin Signaling ◽  
Stem Cell Fate

Targeting Wnt Signaling through Small molecules in Governing Stem Cell Fate and Diseases

2019 ◽  
Vol 19 (3) ◽  
pp. 233-246 ◽  
Author(s):  
Antara Banerjee ◽  
Ganesan Jothimani ◽  
Suhanya Veronica Prasad ◽  
Francesco Marotta ◽  
Surajit Pathak
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Wnt Signaling ◽  
Small Molecules ◽  
Signaling Pathway ◽  
Cell Fate ◽  
Wnt Pathway ◽  
Molecular Mechanisms ◽  
Wnt Signaling Pathway ◽  
Stem Cell Fate

Background:The conserved Wnt/β-catenin signaling pathway is responsible for multiple functions including regulation of stem cell pluripotency, cell migration, self-renewability and cell fate determination. This signaling pathway is of utmost importance, owing to its ability to fuel tissue repair and regeneration of stem cell activity in diverse organs. The human adult stem cells including hematopoietic cells, intestinal cells, mammary and mesenchymal cells rely on the manifold effects of Wnt pathway. The consequences of any dysfunction or manipulation in the Wnt genes or Wnt pathway components result in specific developmental defects and may even lead to cancer, as it is often implicated in stem cell control. It is absolutely essential to possess a comprehensive understanding of the inhibition and/ or stimulation of the Wnt signaling pathway which in turn is implicated in determining the fate of the stem cells.Results:In recent years, there has been considerable interest in the studies associated with the implementation of small molecule compounds in key areas of stem cell biology including regeneration differentiation, proliferation. In support of this statement, small molecules have unfolded as imperative tools to selectively activate and inhibit specific developmental signaling pathways involving the less complex mechanism of action. These compounds have been reported to modulate the core molecular mechanisms by which the stem cells regenerate and differentiate.Conclusion:This review aims to provide an overview of the prevalent trends in the small molecules based regulation of stem cell fate via targeting the Wnt signaling pathway.

The CLAVATA signaling pathway mediating stem cell fate in shoot meristems requires Ca2+as a secondary cytosolic messenger

2016 ◽  
Vol 85 (4) ◽  
pp. 494-506 ◽  
Author(s):  
Hsuan Chou ◽  
Yingfang Zhu ◽  
Yi Ma ◽  
Gerald A. Berkowitz
Keyword(s):  
Stem Cell ◽  
Signaling Pathway ◽  
Cell Fate ◽  
Stem Cell Fate ◽  
Shoot Meristems

scholarly journals Hierarchical macropore/nano surface regulates stem cell fate through a ROCK-related signaling pathway

RSC Advances ◽  
2017 ◽  
Vol 7 (14) ◽  
pp. 8521-8532 ◽  
Author(s):  
Houhua Pan ◽  
Youtao Xie ◽  
Zequan Zhang ◽  
Kai Li ◽  
Dandan Hu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Signaling Pathway ◽  
Cell Fate ◽  
Stem Cell Fate ◽  
Rock Activity

Synergistic cytoskeleton distribution accompanying higher ROCK activity activated FAK-ERK1/2 signaling pathway and promotion of BMSC osteogenesis on hierarchical surface.

scholarly journals GLP-1 Notch - LAG-1 CSL control of the germline stem cell fate is mediated by transcriptional targets lst-1 and sygl-1

2019 ◽  
Author(s):  
Jian Chen ◽  
Ariz Mohammad ◽  
Nanette Pazdernik ◽  
Huiyan Huang ◽  
Beth Bowman ◽  
...  
Keyword(s):  
Stem Cell ◽  
Signaling Pathway ◽  
Cell Fate ◽  
Primary Response ◽  
Secondary Response ◽  
Germline Stem Cell ◽  
Cell Systems ◽  
Stem Cell Fate ◽  
Transcriptional Targets ◽  
C Elegans

AbstractStem cell systems are essential for the development and maintenance of polarized tissues. Intercellular signaling pathways control stem cell systems, where niche cells signal stem cells to maintain the stem cell fate/self renewal and inhibit differentiation. In the C. elegans germline, GLP-1 Notch signaling specifies the stem cell fate. We undertook a comprehensive genome-wide approach to identify transcriptional targets of GLP-1 signaling. We expected primary response target genes to be evident at the intersection of genes identified as directly bound by LAG-1, the C. elegans Notch pathway sequence-specific DNA binding protein, from ChIP-seq experiments, with genes identified as requiring GLP-1 signaling for RNA accumulation, from RNA-seq analysis. Furthermore, we performed a time-course transcriptomics analysis following auxin inducible degradation of LAG-1 to distinguish between genes whose RNA level was a primary or secondary response of GLP-1 signaling. Surprisingly, only lst-1 and sygl-1, the two known target genes of GLP-1 in the germline, fulfilled these criteria, indicating that these two genes are the primary response targets of GLP-1 Notch and may be the sole germline GLP-1 signaling protein-coding transcriptional targets for mediating the stem cell fate. In addition, three secondary response genes were identified based on their timing following loss of LAG-1, their lack of a LAG-1 ChIP-seq peak and that their glp-1 dependent mRNA accumulation could be explained by a requirement for lst-1 and sygl-1 activity. Moreover, our analysis also suggests that the function of the primary response genes lst-1 and sygl-1 can account for the glp-1 dependent peak protein accumulation of FBF-2, which promotes the stem cell fate and, in part, for the spatial restriction of elevated LAG-1 accumulation to the stem cell region.Author SummaryStem cell systems are central to tissue development, homeostasis and regeneration, where niche to stem cell signaling pathways promote the stem cell fate/self-renewal and inhibit differentiation. The evolutionarily conserved GLP-1 Notch signaling pathway in the C. elegans germline is an experimentally tractable system, allowing dissection of control of the stem cell fate and inhibition of meiotic development. However, as in many systems, the primary molecular targets of the signaling pathway in stem cells is incompletely known, as are secondary molecular targets, and this knowledge is essential for a deep understanding of stem cell systems. Here we focus on the identification of the primary transcriptional targets of the GLP-1 signaling pathway that promotes the stem cell fate, employing unbiased multilevel genomic approaches. We identify only lst-1 and sygl-1, two of a number of previously reported targets, as likely the sole primary mRNA transcriptional targets of GLP-1 signaling that promote the germline stem cell fate. We also identify secondary GLP-1 signaling RNA and protein targets, whose expression shows dependence on lst-1 and sygl-1, where the protein targets reinforce the importance of posttranscriptional regulation in control of the stem cell fate.

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