Chondrogenic effect of precartilaginous stem cells following NLS-TAT cell penetrating peptide-assisted transfection of eukaryotic hTGFβ3

2013 ◽  
Vol 114 (11) ◽  
pp. 2588-2594 ◽  
Author(s):  
Xin Guo ◽  
Xiangyu Chu ◽  
Wenkai Li ◽  
Qiyong Pan ◽  
Hongbo You
Keyword(s):  
Stem Cells ◽  
Cell Penetrating Peptide ◽  
Precartilaginous Stem Cells ◽  
Cell Penetrating

scholarly journals Pharmacodynamics of siRNARANKL and Oestrogen Loaded MSNs-CADY on Human Periodontal Ligament Stem Cells with Porphyromonas gingivalis infection

2020 ◽  
Author(s):  
Liang Song ◽  
Xiaojun Shi ◽  
Fengling Hu ◽  
Huijuan Chen ◽  
Bin Xu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Drug Release ◽  
Mesoporous Silica ◽  
Porphyromonas Gingivalis ◽  
Periodontal Ligament ◽  
Mesoporous Silica Nanoparticles ◽  
Cell Penetrating Peptide ◽  
Periodontal Ligament Stem Cells ◽  
Cell Penetrating

Abstract Background: Periodontitis irreversibly invades and destroys periodontal supporting tissues, loses the ability of periodontal regeneration and restoration, and eventually leads to tooth loosening and loss. periodontal ligament stem cells (PDLSCs) hold great promises for periodontal tissue regeneration which was the potential target of periodontitis treatment, siRNARANKL and oestrogen can help PDLSCs maintain normal function, however, it was very difficult for siRNARANKL and oestrogen to get into PDLSCs. Here, Cell penetrating peptide CADY was modified on the surface of siRNARANKL and oestrogen loaded mesoporous silica nanoparticles (MSNs) to carry them into Porphyromonas gingivalis infected PDLSCs, Then further affect the proliferation of PDLSCs. Methods: 120-150 nm Mesoporous silica nanoparticles (MSNs) was prepared, and the biocompatibility, loading capacity and drug release properity were tested; MSNs was modified by penetrating peptide CADY and the prepared MSNs/CADY was loaded with siRNARANKL and oestrogen; In vitro drug release of siRNARANKL/MSNs-CADY and oestrogen/MSNs-CADY was tested by using semi-permeable dialysis bag diffusion; Cellular uptake and internalization of FITC-Labeled MSNs and FITC-Labeled MSNs-CADY was observed by use of Laser confocal microscopy; Finally, the effect of siRNARANKL and oestrogen loaded MSNs-CADY on cell proliferation of Porphyromonas gingivalis infected human periodontal ligament stem cells was tested by MTT assay. Results: according to the results, MSNs-CADY with a concentration of 6.25-200 ug/mL have no toxic to PDLSCs; 24.6 mg oestrogen and 0.5 mM siRNARANKL can be loaded into 1mg of MSNs-CADY; and drug loaded MSNs-CADY nanodrug carriers can release siRNARANKL and oestrogen stably for at least 48 h; After modification with cell penetrating peptide CADY, more MSNs-CADY can be taken by PDLSCs. siRNARANKL/oestrogen/MSNs-CADY can increase the proliferation of PDLSCs significantly. Conclusion: siRNARANKL/oestrogen/MSNs-CADY constructed can significantly improve the cell proliferation of P-gingivalis infected PDLSCs, this nano drug carrier has the potential to be used in PDLSCs -based periodontitis treatment, this work provided a useful theoretical basis and therapeutic ideas for the treatment of periodontitis.

scholarly journals Targeted gene silencing in human embryonic stem cells using cell-penetrating peptide PepFect 14

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Egle-Helene Ervin ◽  
Martin Pook ◽  
Indrek Teino ◽  
Valmar Kasuk ◽  
Annika Trei ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Gene Silencing ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating

DNA-binding Cell-penetrating Peptide-based TRAIL Over-expression in Adipose Tissue-derived Mesenchymal Stem Cells Inhibits Glioma U251MG Growth

2021 ◽  
Vol 41 (6) ◽  
pp. 2859-2866
Author(s):  
JAESIK SHIN ◽  
SOON KOO BAIK ◽  
YONGDAE YOON ◽  
SOONJAE HWANG ◽  
JOON HYUNG SOHN ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Dna Binding ◽  
Cell Penetrating Peptide ◽  
Over Expression ◽  
Cell Penetrating ◽  
Binding Cell

scholarly journals A Novel Cell Penetrating Peptide for the Differentiation of Human Neural Stem Cells

Biomolecules ◽  
2018 ◽  
Vol 8 (3) ◽  
pp. 48 ◽  
Author(s):  
Weili Ma ◽  
Geun-woo Jin ◽  
Paul Gehret ◽  
Neil Chada ◽  
Won Suh
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Cell Penetrating Peptide ◽  
Human Neural Stem Cells ◽  
Cell Penetrating
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