uPA and MMP-2 were involved in self-assembled network formation in a two dimensional co-culture model of bone marrow stromal cells and endothelial cells

2013 ◽  
Vol 114 (3) ◽  
pp. 650-657 ◽  
Author(s):  
Haiyan Li ◽  
Richard Daculsi ◽  
Reine Bareille ◽  
Chantal Bourget ◽  
Joëlle Amedee
Keyword(s):  
Bone Marrow ◽  
Endothelial Cells ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Network Formation ◽  
Marrow Stromal Cells ◽  
Two Dimensional ◽  
Culture Model ◽  
Self Assembled

Development of Self-Assembled, Tissue-Engineered Ligament from Bone Marrow Stromal Cells

2007 ◽  
Vol 0 (0) ◽  
pp. 070108073551001
Author(s):  
Michelle Hairfield-Stein ◽  
Christopher England ◽  
Hyun J. Paek ◽  
Kerry B. Gilbraith ◽  
Robert Dennis ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Self Assembled

scholarly journals PHBV/bioglass composite scaffolds with co-cultures of endothelial cells and bone marrow stromal cells improve vascularization and osteogenesis for bone tissue engineering

RSC Advances ◽  
2017 ◽  
Vol 7 (36) ◽  
pp. 22197-22207 ◽  
Author(s):  
Jun Wu ◽  
Zhi Wu ◽  
Zhenqiang Xue ◽  
Haiyan Li ◽  
Jinbo Liu
Keyword(s):  
Tissue Engineering ◽  
Bone Marrow ◽  
Endothelial Cells ◽  
Bone Tissue Engineering ◽  
Osteogenic Differentiation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Composite Scaffolds ◽  
Paracrine Effects

PHBV + 10% BG composite scaffolds stimulated osteogenic differentiation and angiogenic differentiation of co-cultures of HBMSCs and HUVECs by enhancing paracrine effects between the two types of cells.

scholarly journals Endothelial cells influence the osteogenic potential of bone marrow stromal cells

2009 ◽  
Vol 8 (1) ◽  
pp. 34 ◽  
Author(s):  
Ying Xue ◽  
Zhe Xing ◽  
Sølve Hellem ◽  
Kristina Arvidson ◽  
Kamal Mustafa
Keyword(s):  
Bone Marrow ◽  
Endothelial Cells ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Osteogenic Potential

Development of Self-Assembled, Tissue-Engineered Ligament from Bone Marrow Stromal Cells

2007 ◽  
Vol 13 (4) ◽  
pp. 703-710 ◽  
Author(s):  
Michelle Hairfield-Stein ◽  
Christopher England ◽  
Hyun J. Paek ◽  
Kerry B. Gilbraith ◽  
Robert Dennis ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Self Assembled

Role of neural-cadherin in early osteoblastic differentiation of human bone marrow stromal cells cocultured with human umbilical vein endothelial cells

2010 ◽  
Vol 299 (2) ◽  
pp. C422-C430 ◽  
Author(s):  
Haiyan Li ◽  
Richard Daculsi ◽  
Maritie Grellier ◽  
Reine Bareille ◽  
Chantal Bourget ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Endothelial Cells ◽  
Cell Adhesion ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Umbilical Vein ◽  
Osteoblastic Differentiation ◽  
Marrow Stromal Cells ◽  
Umbilical Vein Endothelial Cells ◽  
Cell Cell

In our previous studies, roles of gap junction and vascular endothelial growth factor in the cross-talking of human bone marrow stromal cells (HBMSCs) and human umbilical vein endothelial cells (HUVECs) have been extensively studied. The present study focused on the investigation of the roles of neural (N)-cadherin in early differentiation of HBMSCs in direct-contact cocultures with HUVECs for 24 and 48 h. Quantitative real-time polymerase chain reaction, immunofluorescence, Western blot, as well as functional studies were applied to perform the studies at both protein and gene levels. Results showed that cocultured cells expressed much higher N-cadherin than monocultured cells after 24 and 48 h of culture. We observed that N-cadherin concentrated in the membrane of cocultured HBMSCs (co-HBMSCs) while distributed within the cytoplasm of monocultured HBMSCs, which indicated that the cell-cell adhesion was improved between cocultured cells. In addition, more β-catenin was found to translocate into the cocultured cells nuclei and more T cell factor-1 (TCF-1) were detected in cocultured cells than in the monocultured cells. Moreover, mRNA levels of early osteoblastic markers including alkaline phosphatase (ALP) and type I collagen (Col-I) of co-HBMSCs were significantly upregulated, whereas neutralization of N-cadherin led to a downregulation of ALP and Col-I in both of the HBMSCs and co-HBMSCs compared with untreated cells. Taking our findings together it can be concluded that cocultures of HBMSCs with HUVECs increased N-cadherin expression and improved cell-cell adhesion. Whether this applies only to osteoprogenitor cells or to all the cell types in the culture will need to be determined by further studies. Subsequently, signaling transduction might be induced with the participation of β-catenin and TCF-1. With the N-cadherin-mediated cell-cell adhesion and signaling transductions, the early osteoblastic differentiation of co-HBMSCs was significantly upregulated.

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