Human Wharton's Jelly stem cell conditioned medium and cell-free lysate inhibit human osteosarcoma and mammary carcinoma cell growth in vitro and in xenograft mice

2012 ◽  
Vol 114 (2) ◽  
pp. 366-377 ◽  
Author(s):  
Kalamegam Gauthaman ◽  
Chui-Yee Fong ◽  
Suganya Arularasu ◽  
Arjunan Subramanian ◽  
Arijit Biswas ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Growth ◽  
Conditioned Medium ◽  
Mammary Carcinoma ◽  
Carcinoma Cell ◽  
Mammary Carcinoma Cell ◽  
Human Osteosarcoma ◽  
Xenograft Mice ◽  
Cell Conditioned Medium

scholarly journals The Potential of a Hair Follicle Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Hair Follicle Regeneration

2020 ◽  
Vol 10 (8) ◽  
pp. 2646
Author(s):  
Keng-Liang Ou ◽  
Yun-Wen Kuo ◽  
Chia-Yu Wu ◽  
Bai-Hung Huang ◽  
Fang-Tzu Pai ◽  
...  
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Hair Follicle ◽  
Conditioned Medium ◽  
Hair Growth ◽  
Hair Regeneration ◽  
Hacat Keratinocyte ◽  
Cell Conditioned Medium

The study elucidated the wound healing and hair regeneration properties of a conditioned medium prepared from the culture of human hair follicle mesenchymal stem cells (HFMSCs). The wound-healing effects of mesenchymal stem cell-conditioned medium (MSC-CM) were tested in vitro using scratch assays co-cultured with HaCaT keratinocyte and monitored through optical microscopy. The cell proliferation of HFMSCs and the HaCaT keratinocyte were observed in the presence of different kinds of drugs including UK5099, sodium L-lactate, lactate dehydrogenase-A, MSC-CM, caffeine, and caffeic acid. The hair regeneration properties were investigated in vivo by administrating the MSC-CM solutions to adult B6 mouse models. For quantification, hematoxylin and eosin staining were performed following euthanasia. In vitro results revealed that MSC-CM promotes dermal cell migrations and enhances proliferation of HFMSCs and HaCaT keratinocytes, demonstrating wound-healing properties. Moreover, when the MSC-CM solutions were applied to the shaved mouse skin, a dark area that expanded overtime was seen. Although no hair growth was found, histological analysis proved that a fat layer thickness increment was found under the mouse’s skin, ultimately projecting the formation of new hair growth. MSC-CM promotes the migration and proliferation of dermal keratinocytes that are beneficial for wound healing and hair growth. It is believed that MSC-CM can potentially serve as the basis of alternative therapeutic applications for wound closure and skin regeneration as well as hair growth stimulation and hair loss prevention in alopecia.

Expression Profiling of Mammary Carcinoma Cell Lines: Correlation of in vitro Invasiveness with Expression of CD24

Tumor Biology ◽  
2002 ◽  
Vol 23 (3) ◽  
pp. 139-145 ◽  
Author(s):  
Sabine Schindelmann ◽  
Jasmin Windisch ◽  
Regina Grundmann ◽  
Rolf Kreienberg ◽  
Robert Zeillinger ◽  
...  
Keyword(s):  
Cell Lines ◽  
Mammary Carcinoma ◽  
Expression Profiling ◽  
Carcinoma Cell ◽  
Mammary Carcinoma Cell ◽  
Carcinoma Cell Lines ◽  
In Vitro Invasiveness
Sign in / Sign up

Export Citation Format

Share Document