Effects of c-Jun and a negative dominant mutation of c-Jun on differentiation and gene expression in lens epithelial cells

1995 ◽  
Vol 58 (2) ◽  
pp. 237-247 ◽  
Author(s):  
Jo Ann Rinaudo ◽  
Emanuel Vacchiano ◽  
Peggy S. Zelenka
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Lens Epithelial Cells ◽  
Dominant Mutation

scholarly journals Identification of Differential Gene Expression Pattern in Lens Epithelial Cells Derived from Cataractous and Noncataractous Lenses of Shumiya Cataract Rat

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Hidetoshi Ishida ◽  
Teppei Shibata ◽  
Yuka Nakamura ◽  
Yasuhito Ishigaki ◽  
Dhirendra P. Singh ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Expression Patterns ◽  
Synergetic Effect ◽  
Gene Expression Pattern ◽  
Lens Opacity ◽  
Lens Epithelial Cells ◽  
Lens Fiber ◽  
Molecular Events ◽  
Old Rats

The Shumiya cataract rat (SCR) is a model for hereditary cataract. Two-thirds of these rats develop lens opacity within 10-11 weeks. Onset of cataract is attributed to the synergetic effect of lanosterol synthase (Lss) and farnesyl-diphosphate farnesyltransferase 1 (Fdft1) mutant alleles that lead to cholesterol deficiency in the lenses, which in turn adversely affects lens biology including the growth and differentiation of lens epithelial cells (LECs). Nevertheless, the molecular events and changes in gene expression associated with the onset of lens opacity in SCR are poorly understood. In the present study, a microarray-based approach was employed to analyze comparative gene expression changes in LECs isolated from the precataractous and cataractous stages of lenses of 5-week-old SCRs. The changes in gene expression observed in microarray results in the LECs were further validated using real-time reverse transcribed quantitative PCR (RT-qPCR) in 5-, 8-, and 10-week-old SCRs. A mild posterior and cortical opacity was observed in 5-week-old rats. Expressions of approximately 100 genes, including the major intrinsic protein of the lens fiber (Mip and Aquaporin 0), deoxyribonuclease II beta (Dnase2B), heat shock protein B1 (HspB1), and crystallin γ (γCry) B, C, and F, were found to be significantly downregulated (0.07-0.5-fold) in rat LECs derived from cataract lenses compared to that in noncataractous lenses (control). Thus, our study was aimed at identifying the gene expression patterns during cataract formation in SCRs, which may be responsible for cataractogenesis in SCR. We proposed that cataracts in SCR are associated with reduced expression of these lens genes that have been reported to be related with lens fiber differentiation. Our findings may have wider implications in understanding the effect of cholesterol deficiency and the role of cholesterol-lowering therapeutics on cataractogenesis.

Differential Gene Expression in the Lens Epithelial Cells from Selenite Injected Rats

2002 ◽  
Vol 74 (2) ◽  
pp. 231-236 ◽  
Author(s):  
Takeshi Nakajima ◽  
Emi Nakajima ◽  
Chiho Fukiage ◽  
Mitsuyoshi Azuma ◽  
Thomas R. Shearer
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Differential Gene Expression ◽  
Lens Epithelial Cells ◽  
Differential Gene

scholarly journals Identification of differential gene expression pattern in lens epithelial cells derived from cataractous and non-cataractous lenses of Shumiya cataract rat

2020 ◽  
Author(s):  
Hidetoshi Ishida ◽  
Teppei Shibata ◽  
Yuka Nakamura ◽  
Yasuhito Ishigaki ◽  
Dhirendra P Singh ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Expression Patterns ◽  
Synergetic Effect ◽  
Gene Expression Pattern ◽  
Lens Opacity ◽  
Lens Epithelial Cells ◽  
Lens Fiber ◽  
Molecular Events ◽  
Lanosterol Synthase

AbstractThe Shumiya cataract rat (SCR) is a model for hereditary cataract. Two-third of these rats develop lens opacity within 10-11-weeks. Onset of cataract is attributed to the synergetic effect of lanosterol synthase (Lss) and farnesyl-diphosphate farnesyltransferase 1 (Fdft1) mutant alleles that lead to cholesterol deficiency in the lenses, which in turn adversely affects lens biology including the growth and differentiation of lens epithelial cells (LECs). Nevertheless, the molecular events and changes in gene expression associated with the onset of lens opacity in SCR is poorly understood. In the present study, a microarray-based approach was employed to analyze comparative gene expression changes in LECs isolated from the pre-cataractous and cataractous stages of lenses of 5-, 8- and 10-week-old SCRs. The changes in gene expression observed in microarray results in the LECs were further validated using real-time PCR and western blot analyses. Lens opacity was not observed in 5-week-old rats. However, histological analysis revealed mild dysplasia in the anterior suture and poorly differentiated fiber cells at the bow region. Expression of approximately 100 genes, including major intrinsic protein of lens fiber (MIP and Aquaporin0), deoxyribonuclease II beta (Dnase2b), heat shock protein B1 (Hspb1), and crystallin γ (γCry) B, C, and F were found to be significantly downregulated (0.07-0.5 fold) in rat LECs derived from cataract lenses compared to that in noncataractous lenses (control). Thus, our study aimed to identify the gene expression patterns during cataract formation in SCRs, which may be responsible for cataractogenesis in SCR. We proposed that mutation in lanosterol synthase was responsible for the downregulation of genes associated with lens fiber differentiation, which in turn leads to the formation of cataract in these rats. Our findings may have wider implications in understanding the effect of cholesterol deficiency and the role of cholesterol-lowering therapeutics on cataractogenesis.

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