Detection and localization of actin mRNA isoforms in chicken muscle cells by in situ hybridization using biotinated oligonucleotide probes

1990 ◽  
Vol 44 (4) ◽  
pp. 241-252 ◽  
Author(s):  
Krishan L. Taneja ◽  
Robert H. Singer
Keyword(s):  
In Situ Hybridization ◽  
Muscle Cells ◽  
Oligonucleotide Probes ◽  
Mrna Isoforms ◽  
Chicken Muscle ◽  
Actin Mrna ◽  
Detection And Localization

Actin mRNA isoforms are differentially sorted in normal osteoblasts and sorting is altered in osteoblasts from a skeletal mutation in the rat

1998 ◽  
Vol 111 (9) ◽  
pp. 1287-1292 ◽  
Author(s):  
H. Watanabe ◽  
E.H. Kislauskis ◽  
C.A. Mackay ◽  
A. Mason-Savas ◽  
S.C. Marks
Keyword(s):  
In Situ Hybridization ◽  
Bone Resorption ◽  
Cell Types ◽  
Mrna Isoforms ◽  
Actin Isoforms ◽  
Beta Actin ◽  
Cell Processes ◽  
Actin Mrna ◽  
Actin Isoform

Actin isoform sorting has been shown to occur in a variety of cell types in culture. To this list we add osteoblasts, in which we show by in situ hybridization that beta-actin is distributed primarily in cell processes and on one side of the nucleus and gamma-actin has a perinuclear distribution. Osteoblasts from the skeletal mutation toothless (tl), evaluated under identical conditions, fail to sort these actin isoforms differentially and exhibit diffuse labeling as their major manifestation. Northern analyses of actin mRNAs showed no differences between normal and mutant cultures. Shortened osteoblast life span and an inability to direct osteoclast-mediated bone resorption have recently been demonstrated in tl mutants. The present results suggest that a failure of osteoblasts to sort actin mRNAs may be related to one or both of these pathological manifestations in this mutation and represent, to our knowledge, the first correlation of an actin mRNA-sorting abnormality with a mammalian disease.

Detection and localization of human papillomavirus DNA in human genital condylomas by in situ hybridization with biotinylated probes

1985 ◽  
Vol 16 (3) ◽  
pp. 265-273 ◽  
Author(s):  
Anna Marie Beckmann ◽  
David Myerson ◽  
Janet R. Daling ◽  
Nancy B. Kiviat ◽  
Cecilia M. Fenoglio ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Human Papillomavirus ◽  
Detection And Localization ◽  
Papillomavirus Dna ◽  
Biotinylated Probes

Visualization of sporopollenin-containing pathogenic green micro-alga Prototheca wickerhamii by fluorescent in situ hybridization (FISH)

2009 ◽  
Vol 55 (4) ◽  
pp. 465-472 ◽  
Author(s):  
Ryohei Ueno
Keyword(s):  
Cell Wall ◽  
In Situ Hybridization ◽  
Cell Walls ◽  
Fluorescent In Situ Hybridization ◽  
Rapid Identification ◽  
Oligonucleotide Probes ◽  
Logarithmic Growth Phase ◽  
Prototheca Wickerhamii ◽  
Algal Genus

Fluorescent in situ hybridization (FISH) using taxon-specific, rRNA-targeted oligonucleotide probes is one of the most powerful tools for the rapid identification of harmful microorganisms. However, eukaryotic algal cells do not always allow FISH probes to permeate over their cell walls. Members of the pathogenic micro-algal genus Prototheca are characterized by their distinctive cell-wall component, sporopollenin, an extremely tough biopolymer that resists acid and alkaline hydrolysis, enzyme attack, and acetolysis. To our knowledge, there has been no report of the successful permeation by the oligonucleotide probes over the cell walls of unicellular green micro-algae, which contain sporopollenin. The DNA probes passed through the cell wall of Prototheca wickerhamii after treating the algal cells with cetyltrimethylammonium bromide (CTAB). Most cells in the middle logarithmic growth phase culture fluoresced when hybridized with the rRNA-targeted universal probe for eukaryotes, though individual cells included in this culture differed in the level of cell-wall vulnerability to attack by the polysaccharide-degrading enzyme, thus reflecting the different stages of the life cycle. This is the first report regarding the visualization of sporopollenin-containing, green micro-algal cells by FISH.

scholarly journals Microstructure of Anaerobic Granules Bioaugmented with Desulfitobacterium frappieri PCP-1

2002 ◽  
Vol 68 (8) ◽  
pp. 4035-4043 ◽  
Author(s):  
M. Lanthier ◽  
B. Tartakovsky ◽  
R. Villemur ◽  
G. DeLuca ◽  
S. R. Guiot
Keyword(s):  
Growth Rate ◽  
In Situ Hybridization ◽  
Outer Layer ◽  
Specific Growth ◽  
Anaerobic Sludge ◽  
Oligonucleotide Probes ◽  
Reactor Operation ◽  
Mathematical Simulations ◽  
Successful Colonization

ABSTRACT Oligonucleotide probes were used to study the structure of anaerobic granular biofilm originating from a pentachlorophenol-fed upflow anaerobic sludge bed reactor augmented with Desulfitobacterium frappieri PCP-1. Fluorescence in situ hybridization demonstrated successful colonization of anaerobic granules by strain PCP-1. Scattered microcolonies of strain PCP-1 were detected on the biofilm surface after 3 weeks of reactor operation, and a dense outer layer of strain PCP-1 was observed after 9 weeks. Hybridization with probes specific for Eubacteria and Archaea probes showed that Eubacteria predominantly colonized the outer layer, while Archaea were observed in the granule interior. Mathematical simulations showed a distribution similar to that observed experimentally when using a specific growth rate of 2.2 day−1 and a low bacterial diffusion of 10−7 dm2 day−1. Also, the simulations showed that strain PCP-1 proliferation in the outer biofilm layer provided excellent protection of the biofilm from pentachlorophenol toxicity.

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