Characterization of tumors produced by signal peptide-basic fibroblast growth factor-transformed cells

1989 ◽  
Vol 39 (1) ◽  
pp. 13-23 ◽  
Author(s):  
Snezna Rogelj ◽  
Robert A. Weinberg ◽  
Paul Fanning ◽  
Michael Klagsbrun
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Signal Peptide ◽  
Transformed Cells ◽  
Fibroblast Growth

scholarly journals Synthesis and Characterization of a Homogeneous Chemical Conjugate Between Basic Fibroblast Growth Factor and Saporin

1995 ◽  
Vol 234 (3) ◽  
pp. 706-713 ◽  
Author(s):  
Ying Ji Buechler ◽  
Barbara A. Sosnowski ◽  
Kimberly D. Victor ◽  
Zahra Parandoosh ◽  
Stuart J. Bussell ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Synthesis And Characterization ◽  
Fibroblast Growth ◽  
Chemical Conjugate ◽  
Homogeneous Chemical

scholarly journals A growth factor-repressible gene associated with protein kinase C-mediated inhibition of adipocyte differentiation.

1988 ◽  
Vol 107 (1) ◽  
pp. 279-286 ◽  
Author(s):  
M Navre ◽  
G M Ringold
Keyword(s):  
Protein Kinase C ◽  
Growth Factor ◽  
Protein Kinase ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Phorbol Esters ◽  
Adipocyte Differentiation ◽  
Fibroblast Growth ◽  
Kinase C

The conversion of determined adipoblasts to fully differentiated adipocytes requires appropriate environmental conditions. A strict dependence on cell confluence and a facilitation by glucocorticoid hormones have previously been described. We have found that agents that are capable of activating protein kinase C, such as basic fibroblast growth factor and phorbol esters, inhibit the differentiation of the adipogenic cell line TA1 without stimulating cell growth. Here we describe the sequence and characterization of a cDNA (clone 5) that detects an RNA, the expression of which is enhanced by glucocorticoids and increasing cell density. In contrast, activators of protein kinase C including basic fibroblast growth factor, phorbol esters, and synthetic diacylglycerols inhibit clone 5 gene expression. It appears that clone 5 expression is closely linked to environmental and hormonal factors that promote the differentiation of adipogenic cells.

scholarly journals Transformation of mouse BALB/c 3T3 cells with human basic fibroblast growth factor cDNA.

1988 ◽  
Vol 8 (2) ◽  
pp. 588-594 ◽  
Author(s):  
R Sasada ◽  
T Kurokawa ◽  
M Iwane ◽  
K Igarashi
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Cellular Transformation ◽  
Soft Agar ◽  
Transformed Cells ◽  
Fibroblast Growth ◽  
Morphological Transformation ◽  
Phenotypic Alteration ◽  
Autocrine Stimulation

The expression of human basic fibroblast growth factor (bFGF) cDNA in mouse BALB/c 3T3 clone A31 cells induced morphological transformation. These transformed cells grew well and reached more than a sixfold-higher saturation density than parental A31 cells even in serum-free medium. They were able to form colonies in soft agar. The phenotypic alteration in the transformed cells was reversed by the addition of anti-human bFGF antibodies to the medium. These results suggest that the cellular transformation mediated by bFGF is caused by autocrine stimulation with secreted bFGF molecules.

scholarly journals Characterization of the signalling pathways involved in ATP and basic fibroblast growth factor-induced astrogliosis

1997 ◽  
Vol 121 (8) ◽  
pp. 1692-1699 ◽  
Author(s):  
Chiara Bolego ◽  
Stefania Ceruti ◽  
Roberta Brambilla ◽  
Lina Puglisi ◽  
Flaminio Cattabeni ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Signalling Pathways ◽  
Fibroblast Growth

Purification and characterization of the 210-amino acid recombinant basic fibroblast growth factor form (FGF-2)

FEBS Letters ◽  
1994 ◽  
Vol 349 (1) ◽  
pp. 23-28 ◽  
Author(s):  
Véronique Patry ◽  
Béatrix Bugler ◽  
François Amalric ◽  
Jean-Claude Promé ◽  
Hervé Prats
Keyword(s):  
Amino Acid ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Fibroblast Growth ◽  
Purification And Characterization ◽  
Factor Form

Characterization of an endooligopeptidase A-like protein in PC12 cells: Activity modulation by cAMP but not by basic fibroblast growth factor

1995 ◽  
Vol 57 (2) ◽  
pp. 311-320 ◽  
Author(s):  
Emer S. Ferro ◽  
Denise V. Tambourgy ◽  
Patricia A. E. Abreu ◽  
Antonio C. M. Camargo ◽  
Isaias Raw ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Pc12 Cells ◽  
Basic Fibroblast Growth Factor ◽  
Fibroblast Growth

scholarly journals Plant-Produced Basic Fibroblast Growth Factor (bFGF) Promotes Cell Proliferation and Collagen Production

2020 ◽  
Vol 07 (04) ◽  
pp. e150-e157
Author(s):  
Kaewta Rattanapisit ◽  
Angkana Jantimaporn ◽  
Pornjira Kaewpungsup ◽  
Balamurugan Shanmugaraj ◽  
Prasit Pavasant ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Signal Peptide ◽  
Dermal Fibroblast ◽  
Biologically Active ◽  
Vector System ◽  
Fibroblast Growth ◽  
Collagen Production

AbstractHuman fibroblast growth factor regulates a broad spectrum of biological functions, including cell proliferation and tissue differentiation, and has a wider application in tissue engineering. Here, we described the production of human basic fibroblast growth factor in plants by using a geminiviral vector system. In this study, we transiently expressed basic fibroblast growth factor containing a C-terminus 8X-Histidine with and without a barley alpha amylase signal peptide in Nicotiana benthamiana. The expression level of basic fibroblast growth factor without the signal peptide was found to be higher than the basic fibroblast growth factor with the signal peptide. Further, the recombinant basic fibroblast growth factor was purified from the plant crude extract by two-step purification viz., ammonium sulfate precipitation and Ni-affinity chromatography. Our results demonstrated that the purified plant-produced basic fibroblast growth factor was biologically active and promotes the proliferation of human periodontal ligament stem cells and human follicle dermal papilla cells in vitro. Moreover, the plant-produced basic fibroblast growth factor also induced collagen production in human dermal fibroblast cells. Our results suggest the potential use of plant-produced basic fibroblast growth factor as an antiaging and hair growth-promoting agent in the cosmetic industry.

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