Interferon-?-(IFN) producing CHO cell lines are resistant to the antiproliferative activity of IFN: A correlation with gene expression

1988 ◽  
Vol 38 (4) ◽  
pp. 269-278 ◽  
Author(s):  
M. van Heuvel ◽  
M. Govaert-Siemerink ◽  
I. J. Bosveld ◽  
E. G. Zwarthoff ◽  
J. Trapman
Keyword(s):  
Gene Expression ◽  
Cell Lines ◽  
Antiproliferative Activity ◽  
Cho Cell

Identification of the limitations on recombinant gene expression in CHO cell lines with varying luciferase production rates

2009 ◽  
Vol 102 (6) ◽  
pp. 1593-1602 ◽  
Author(s):  
Emma J. Mead ◽  
Lesley M. Chiverton ◽  
C. Mark Smales ◽  
Tobias von der Haar
Keyword(s):  
Gene Expression ◽  
Cell Lines ◽  
Cho Cell ◽  
Production Rates ◽  
Recombinant Gene Expression ◽  
Recombinant Gene

scholarly journals CHO-S Antibody Titers >1 Gram/Liter Using Flow Electroporation-Mediated Transient Gene Expression followed by Rapid Migration to High-Yield Stable Cell Lines

2014 ◽  
Vol 20 (4) ◽  
pp. 545-551 ◽  
Author(s):  
Krista Steger ◽  
James Brady ◽  
Weili Wang ◽  
Meg Duskin ◽  
Karen Donato ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Lines ◽  
Early Stage ◽  
Transient Gene Expression ◽  
Genetically Engineered ◽  
Expression Vectors ◽  
High Yield ◽  
Stable Cell Line ◽  
Cho Cell ◽  
Antibody Titers

In recent years, researchers have turned to transient gene expression (TGE) as an alternative to CHO stable cell line generation for early-stage antibody development. Despite advances in transfection methods and culture optimization, the majority of CHO-based TGE systems produce insufficient antibody titers for extensive use within biotherapeutic development pipelines. Flow electroporation using the MaxCyte STX Scalable Transfection System is a highly efficient, scalable means of CHO-based TGE for gram-level production of antibodies without the need for specialized expression vectors or genetically engineered CHO cell lines. CHO cell flow electroporation is easily scaled from milligram to multigram quantities without protocol reoptimization while maintaining transfection performance and antibody productivity. In this article, data are presented that demonstrate the reproducibility, scalability, and antibody production capabilities of CHO-based TGE using the MaxCyte STX. Data show optimization of posttransfection parameters such as cell density, media composition, and feed strategy that result in secreted antibody titers >1 g/L and production of multiple grams of antibody within 2 weeks of a single CHO-S cell transfection. In addition, data are presented to demonstrate the application of scalable electroporation for the rapid generation of high-yield stable CHO cell lines to bridge the gap between early- and late-stage antibody development activities.

DNA Methylation Inhibites ANXA1 Gene Expression in Nasopharyngeal Carcinoma Cell Lines*

2009 ◽  
Vol 36 (10) ◽  
pp. 1319-1326 ◽  
Author(s):  
Shuang-Xiang TAN ◽  
Rui-Cheng HU ◽  
Ai-Guo DAI ◽  
Cen-E TANG ◽  
Hong YI ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Nasopharyngeal Carcinoma ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Nasopharyngeal Carcinoma Cell ◽  
Carcinoma Cell Lines
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