Phenotypic differences in subclones and long-term cultures of clonally derived rat bone cell lines

1986 ◽  
Vol 31 (2) ◽  
pp. 153-169 ◽  
Author(s):  
Carlton G. Bellows ◽  
Jaro Sodek ◽  
Kam-Ling Yao ◽  
Jane E. Aubin
Keyword(s):  
Cell Lines ◽  
Bone Cell ◽  
Phenotypic Differences ◽  
Rat Bone

scholarly journals Apoferritin/Vandetanib Association Is Long-Term Stable but Does Not Improve Pharmacological Properties of Vandetanib

2021 ◽  
Vol 22 (8) ◽  
pp. 4250
Author(s):  
Kateřina Jáklová ◽  
Tereza Feglarová ◽  
Simona Rex ◽  
Zbyněk Heger ◽  
Tomáš Eckschlager ◽  
...  
Keyword(s):  
Cell Lines ◽  
Targeted Delivery ◽  
Medullary Thyroid Cancer ◽  
Kinase Inhibitor ◽  
Medullary Thyroid ◽  
Long Term Stability ◽  
Carcinoma Cell Lines ◽  
Average Size ◽  
Thyroid Carcinoma Cell

A tyrosine kinase inhibitor, vandetanib (Van), is an anticancer drug affecting the signaling of VEGFR, EGFR and RET protooncogenes. Van is primarily used for the treatment of advanced or metastatic medullary thyroid cancer; however, its usage is significantly limited by side effects, particularly cardiotoxicity. One approach to minimize them is the encapsulation or binding of Van in- or onto a suitable carrier, allowing targeted delivery to tumor tissue. Herein, we constructed a nanocarrier based on apoferritin associated with Van (ApoVan). Based on the characteristics obtained by analyzing the average size, the surface ζ-potential and the polydispersive index, ApoVan nanoparticles exhibit long-term stability and maintain their morphology. Experiments have shown that ApoVan complex is relatively stable during storage. It was found that Van is gradually released from its ApoVan form into the neutral environment (pH 7.4) as well as into the acidic environment (pH 6.5). The effect of free Van and ApoVan on neuroblastoma and medullary thyroid carcinoma cell lines revealed that both forms were toxic in both used cell lines, and minimal differences between ApoVan and Van were observed. Thus, we assume that Van might not be encapsulated into the cavity of apoferritin, but instead only binds to its surface.

scholarly journals Patient-individual cancer cell lines and tissue analysis delivers no evidence for a role of DNA virus infection on colorectal cancer oncogenesis

2019 ◽  
Author(s):  
Michael Gock ◽  
Marcel Kordt ◽  
Stephanie Matschos ◽  
Christina S. Mullins ◽  
Michael Linnebacher
Keyword(s):  
Cell Lines ◽  
Molecular Subtypes ◽  
Morphological Changes ◽  
Tissue Analysis ◽  
Oncogenic Viruses ◽  
Dna Viruses ◽  
Viral Particles ◽  
Specific Sequences

Abstract Background Several DNA viruses are highly suspicious to have oncogenic effects in humans. This study investigates the presence of potentially oncogenic viruses such as SV40, JCV, BKV and EBV in patient-derived colorectal carcinoma (CRC) cells typifying all molecular subtypes of CRC. Methods Sample material (gDNA and cDNA) of a total of 49 patient-individual CRC cell lines and corresponding primary material from 11 patients, including normal, tumor-derived and metastasis-derived tissue were analyzed for sequences of SV40, JVC, BKV and EBV using endpoint PCR. In addition, the susceptibility of CRC cells to JCV and BKV was examined using a long-term cultivation approach of patient-individual cells in the presence of viruses. Results No virus-specific sequences could be detected in all specimens. Likewise, no morphological changes were observed and no evidence for viral infection or integration could be provided after long term CRC cell cultivation in presence of viral particles. Conclusions In summary, the presented data suggest that there is no direct correlation between tumorigenesis and viral load and consequently no evidence for a functional role of the DNA viruses included into this analysis in CRC development.

scholarly journals Upstream Regulatory Role for XIAP in Receptor-Mediated Apoptosis

2004 ◽  
Vol 24 (16) ◽  
pp. 7003-7014 ◽  
Author(s):  
John C. Wilkinson ◽  
Enrique Cepero ◽  
Lawrence H. Boise ◽  
Colin S. Duckett
Keyword(s):  
Cell Death ◽  
Cell Lines ◽  
Death Receptor ◽  
Full Length ◽  
Proliferative Capacity ◽  
Term Survival ◽  
Truncation Mutant ◽  
Short And Long Term ◽  
Induced Apoptosis

ABSTRACT X-linked inhibitor of apoptosis (XIAP) is an endogenous inhibitor of cell death that functions by suppressing caspases 3, 7, and 9. Here we describe the establishment of Jurkat-derived cell lines stably overexpressing either full-length XIAP or a truncation mutant of XIAP that can only inhibit caspase 9. Characterization of these cell lines revealed that following CD95 activation full-length XIAP supported both short- and long-term survival as well as proliferative capacity, in contrast to the truncation mutant but similar to Bcl-xL. Full-length XIAP was also able to inhibit CD95-mediated caspase 3 processing and activation, the mitochondrial release of cytochrome c and Smac/DIABLO, and the loss of mitochondrial membrane potential, whereas the XIAP truncation mutant failed to prevent any of these cell death events. Finally, suppression of XIAP levels by RNA interference sensitized Bcl-xL-overexpressing cells to death receptor-induced apoptosis. These data demonstrate for the first time that full-length XIAP inhibits caspase activation required for mitochondrial amplification of death receptor signals and that, by acting upstream of mitochondrial activation, XIAP supports the long-term proliferative capacity of cells following CD95 stimulation.

Long-term adaptation of breast tumor cell lines to high concentrations of nitric oxide

Tumor Biology ◽  
2010 ◽  
Vol 31 (4) ◽  
pp. 267-275 ◽  
Author(s):  
Benjamin J. Vesper ◽  
Kim M. Elseth ◽  
Gabor Tarjan ◽  
G. Kenneth Haines ◽  
James A. Radosevich
Keyword(s):  
Nitric Oxide ◽  
Tumor Cell ◽  
Cell Lines ◽  
Breast Tumor ◽  
Tumor Cell Lines ◽  
Breast Tumor Cell ◽  
High Concentrations

Retinoic acid effects on an SV-40 large T antigen immortalized adult rat bone cell line

1999 ◽  
Vol 179 (3) ◽  
pp. 267-275 ◽  
Author(s):  
Marie-Helene Lafage-Proust ◽  
Gregg Wesolowski ◽  
Matthias Ernst ◽  
Gideon A. Rodan ◽  
Sevgi B. Rodan
Keyword(s):  
Retinoic Acid ◽  
Cell Line ◽  
Bone Cell ◽  
T Antigen ◽  
Large T Antigen ◽  
Adult Rat ◽  
Rat Bone

Structure Activity Relationships of Antiproliferative Rocaglamide Derivatives from Aglaia Species (Meliaceae)

1999 ◽  
Vol 54 (1-2) ◽  
pp. 55-60 ◽  
Author(s):  
Frank I. Bohnenstengel ◽  
Klaus G. Steube ◽  
Corinna Meyer ◽  
Bambang W. Nugroho ◽  
Pham D. Hung ◽  
...  
Keyword(s):  
Cell Lines ◽  
Potential Role ◽  
Human Cancer ◽  
Experimental Medicine ◽  
Dependent Manner ◽  
Pyran Ring ◽  
Human Cancer Cell Lines ◽  
Structure Activity ◽  
High Doses

Eleven rocaglamide derivatives (cyclopentatetrahydrobenzofurans) and one structurally related aglain congener all isolated from different Aglaia species (Meliaceae) were tested for growth inhibiting properties using the human cancer cell lines MONO-MAC-6 and MEL-JUSO. Proliferation of both cell lines was efficiently inhibited in a dose and compound dependent manner. Applying a MTT-Assay, the IC50 of the most active compound didesmethyl-rocaglamide (1) was observed at 0.002 and 0.006 μg/ml (0.004 and 0.013 μM) depending on the cell line investigated. Bulky aminoacyl substituents at C-2, acetylation of the OH substituent at C-1 or insertion of a OH or OMe substituent at C-3 ’of the rocaglamide skeleton all diminished the activity of the compounds investigated. The aglain derivative 12 was inactive up to a concentration of 3 μg/ml (4.6 μᴍ) . This loss of activity is assumed to be mainly due to the presence of a pyran ring in the aglains vs. a furan ring as found in rocaglamide derivatives. Rocaglamide derivatives may act primarily by inhibition of cell proliferation as evidenced by the absence of a significant cytotoxic effect in long-term cultures of MONO-MAC-6 cells treated with high doses of didesmethylrocaglamide. Our data suggest that rocaglamide derivatives could exert a potential role in the treatment of malignant diseases and are worth to be investigated in further studies of experimental medicine and pharmacology

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