Interaction of the insulin receptor kinase with serine/threonine kinases in vitro

1985 ◽  
Vol 28 (2) ◽  
pp. 171-182 ◽  
Author(s):  
Hans U. Haring ◽  
Morris F. White ◽  
C. Ronald Kahn ◽  
Zafeer Ahmad ◽  
Anna A. DePaoli-Roach ◽  
...  
Keyword(s):  
Insulin Receptor ◽  
Receptor Kinase ◽  
Threonine Kinases ◽  
Insulin Receptor Kinase

scholarly journals Tyrosine phosphorylation of two cytosolic proteins of 50 kDa and 35 kDa in rat liver by insulin-receptor kinase in vitro

1987 ◽  
Vol 248 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Y C Kwok ◽  
C C Yip
Keyword(s):  
Insulin Receptor ◽  
Rat Liver ◽  
Growth Factor Receptor ◽  
Insulin Receptors ◽  
Artificial Substrates ◽  
Receptor Kinase ◽  
Insulin Receptor Tyrosine Kinase ◽  
Cytosolic Proteins ◽  
Insulin Receptor Kinase

Insulin-receptor tyrosine kinase can phosphorylate a variety of artificial substrates in vitro. Its physiological substrate(s), however, remains unknown. In the present study, we show that immobilized insulin receptors phosphorylate tyrosine residues of two cytosolic proteins of 50 kDa and 35 kDa in rat liver. Phosphorylation of these two proteins required Mn2+- or Mg2+-ATP as the phosphate donor. Phosphorylation was time- and temperature-dependent. Furthermore, the rate of phosphorylation of the two proteins was related to the autophosphorylated state of the insulin receptor. The pI of the phosphorylated 50 kDa and 35 kDa proteins was 5.4 and 5.6 respectively. These proteins were present in low abundance. They were not related to each other, nor to the insulin receptor, as demonstrated by in-gel proteolytic digestion and by immunoprecipitation using antibodies produced against them. They were specific substrates for the insulin receptor kinase, since they were not phosphorylated by epidermal-growth-factor-receptor kinase. These observations suggest that the 50 kDa and 35 kDa cytosolic proteins may be endogenous substrates for the insulin-receptor kinase.

Tissue Specific Differences in the Insulin Receptor Kinase Activated in Vitro and in Vivo*

Endocrinology ◽  
1988 ◽  
Vol 122 (2) ◽  
pp. 427-437 ◽  
Author(s):  
CHARLES F. BURANT ◽  
MARY K. TREUTELAAR ◽  
MARIA G. BUSE
Keyword(s):  
Insulin Receptor ◽  
Receptor Kinase ◽  
Tissue Specific ◽  
Insulin Receptor Kinase
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