Isorhamnetin inhibits H2O2-induced activation of the intrinsic apoptotic pathway in H9c2 cardiomyocytes through scavenging reactive oxygen species and ERK inactivation

2012 ◽  
Vol 113 (2) ◽  
pp. 473-485 ◽  
Author(s):  
Bing Sun ◽  
Gui-Bo Sun ◽  
Jing Xiao ◽  
Rong-Chang Chen ◽  
Xin Wang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Intrinsic Apoptotic Pathway ◽  
Apoptotic Pathway ◽  
H9c2 Cardiomyocytes

Novel Gold and Silver Carbene Complexes Exert Antitumor Effects Triggering the Reactive Oxygen Species Dependent Intrinsic Apoptotic Pathway

ChemMedChem ◽  
2017 ◽  
Vol 12 (24) ◽  
pp. 2054-2065 ◽  
Author(s):  
Domenico Iacopetta ◽  
Annaluisa Mariconda ◽  
Carmela Saturnino ◽  
Anna Caruso ◽  
Giuseppe Palma ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Intrinsic Apoptotic Pathway ◽  
Antitumor Effects ◽  
Apoptotic Pathway ◽  
Carbene Complexes

scholarly journals Mitochondria-Ros Crosstalk in the Control of Cell Death and Aging

2012 ◽  
Vol 2012 ◽  
pp. 1-17 ◽  
Author(s):  
Saverio Marchi ◽  
Carlotta Giorgi ◽  
Jan M. Suski ◽  
Chiara Agnoletto ◽  
Angela Bononi ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Death ◽  
Redox State ◽  
Balance Of Power ◽  
Molecular Pathways ◽  
Oxygen Species ◽  
Intrinsic Apoptotic Pathway ◽  
Apoptotic Pathway ◽  
Induce Cell Death

Reactive oxygen species (ROS) are highly reactive molecules, mainly generated inside mitochondria that can oxidize DNA, proteins, and lipids. At physiological levels, ROS function as “redox messengers” in intracellular signalling and regulation, whereas excess ROS induce cell death by promoting the intrinsic apoptotic pathway. Recent work has pointed to a further role of ROS in activation of autophagy and their importance in the regulation of aging. This review will focus on mitochondria as producers and targets of ROS and will summarize different proteins that modulate the redox state of the cell. Moreover, the involvement of ROS and mitochondria in different molecular pathways controlling lifespan will be reported, pointing out the role of ROS as a “balance of power,” directing the cell towards life or death.

scholarly journals Hesperidin Extracted from Citrus reticulata Blanco Protects Cardiac Mitochondria Against Hypoxia/Reoxygenation Injury

2021 ◽  
Vol 37 (4) ◽  
Author(s):  
Vu Thi Thu ◽  
Phuong Thien Thuong
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Cell Group ◽  
Control Group ◽  
Citrus Reticulata ◽  
Oxygen Species ◽  
Cardiac Mitochondria ◽  
Citrus Reticulata Blanco ◽  
H9c2 Cardiomyocytes ◽  
Hypoxia Reoxygenation

This study was conducted to evaluate the protective effect of Hesperdin (Hes) extracted from Citrus reticulata Blanco on cardiac mitochondria in hypoxia/reoxygenation (HR) injury in vitro. Methods: H9C2 cardiomyocytes were cultured under normal (control), HR, and treatment conditions. The reactive oxygen species and calcium levels in experimental groups were analyzed by using suitable fluorescence kits. Results: The obtained results showed that the addition of Hes at dose of  0,01562 mg/mL sharply decreased the mitochondrial oxidative stress of H9C2 cells under HR conditions. In particular, Hes showed the remarkable efficiency in maintaing cellular calcium levels. In HR-exposed H9C2 cell group, the hydrogen peroxide and superoxide levels were highly increased compared to those in control group (1,54±0,06 and 1,74±0,38, p<0,05). HR also strongly induced the elevation of cytosolic Ca²⁺ and mitochondial Ca²⁺ of H9C2 cardiomyocytes with the values were 1,96±0,05% and 1,62±0,33 (ratio to control, p<0,05), respectively. Interestingly, post-hypoxic supplementation of Hes effectivelly abolished the negative incresement of these indicators with the lower levels of reactive oxygen species and the better modulation of Ca²⁺ homeostasis. Conclusion: The present results are pilot data on the effects of Hes in protecting cardiac mitochondria against HR injury.

Sign in / Sign up

Export Citation Format

Share Document