Effect of dual-specificity protein phosphatase 5 on pluripotency maintenance and differentiation of mouse embryonic stem cells

2011 ◽  
Vol 112 (11) ◽  
pp. 3185-3193 ◽  
Author(s):  
Qi Chen ◽  
Yang Zhou ◽  
Xiaoli Zhao ◽  
Ming Zhang
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Protein Phosphatase ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Protein Phosphatase 5 ◽  
Dual Specificity ◽  
Dual Specificity Protein Phosphatase ◽  
Specificity Protein ◽  
Pluripotency Maintenance

Mechanisms of pluripotency maintenance in mouse embryonic stem cells

2016 ◽  
Vol 74 (10) ◽  
pp. 1805-1817 ◽  
Author(s):  
Chen-Yun Chen ◽  
Yuan-Yuan Cheng ◽  
Christopher Y. T. Yen ◽  
Patrick C. H. Hsieh
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Pluripotency Maintenance

Slc25a36 modulates pluripotency of mouse embryonic stem cells by regulating mitochondrial function and glutathione level

2019 ◽  
Vol 476 (11) ◽  
pp. 1585-1604 ◽  
Author(s):  
Yanli Xin ◽  
Yanliang Wang ◽  
Liang Zhong ◽  
Bingbo Shi ◽  
Hui Liang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cells ◽  
Mitochondrial Dysfunction ◽  
Cell Fate ◽  
Mitochondrial Function ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Glutathione Metabolism ◽  
Pluripotency Maintenance

Abstract Mitochondria play a central role in the maintenance of the naive state of embryonic stem cells. Many details of the mechanism remain to be fully elucidated. Solute carrier family 25 member 36 (Slc25a36) might regulate mitochondrial function through transporting pyrimidine nucleotides for mtDNA/RNA synthesis. Its physical role in this process remains unknown; however, Slc25a36 was recently found to be highly expressed in naive mouse embryonic stem cells (mESCs). Here, the function of Slc25a36 was characterized as a maintenance factor of mESCs pluripotency. Slc25a36 deficiency (via knockdown) has been demonstrated to result in mitochondrial dysfunction, which induces the differentiation of mESCs. The expression of key pluripotency markers (Pou5f1, Sox2, Nanog, and Utf1) decreased, while that of key TE genes (Cdx2, Gata3, and Hand1) increased. Cdx2-positive cells emerged in Slc25a36-deficient colonies under trophoblast stem cell culture conditions. As a result of Slc25a36 deficiency, mtDNA of knockdown cells declined, leading to impaired mitochondria with swollen morphology, decreased mitochondrial membrane potential, and low numbers. The key transcription regulators of mitochondrial biogenesis also decreased. These results indicate that mitochondrial dysfunction leads to an inability to support the pluripotency maintenance. Moreover, down-regulated glutathione metabolism and up-regulated focal adhesion reinforced and stabilized the process of differentiation by separately enhancing OCT4 degradation and promoting cell spread. This study improves the understanding of the function of Slc25a36, as well as the relationship of mitochondrial function with naive pluripotency maintenance and stem cell fate decision.

The effects of gelatin–dopamine coating on polydimethylsiloxane substrates on pluripotency maintenance and myocardial differentiation of cultured mouse embryonic stem cells

2016 ◽  
Vol 4 (48) ◽  
pp. 7961-7973 ◽  
Author(s):  
Jiayin Fu ◽  
Kai Yun Quek ◽  
Yon Jin Chuah ◽  
Chee Seong Lim ◽  
Changjiang Fan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Pluripotency Maintenance ◽  
Myocardial Differentiation

Gelatin–dopamine coating to improve ESC culture and myocardial differentiation on PDMS substrates.

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