Effect of cleidocranial dysplasia-related novel mutation of RUNX2 on characteristics of dental pulp cells and tooth development

2010 ◽  
Vol 111 (6) ◽  
pp. 1473-1481 ◽  
Author(s):  
DongYing Xuan ◽  
Xi Sun ◽  
YuXia Yan ◽  
BaoYi Xie ◽  
PingPing Xu ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Tooth Development ◽  
Novel Mutation ◽  
Cleidocranial Dysplasia ◽  
Dental Pulp Cells ◽  
Pulp Cells

The location and characteristics of two populations of dental pulp cells affect tooth development

2009 ◽  
Vol 117 (2) ◽  
pp. 113-121 ◽  
Author(s):  
Yoshinori Sumita ◽  
Shuhei Tsuchiya ◽  
Izumi Asahina ◽  
Hideaki Kagami ◽  
Masaki J. Honda
Keyword(s):  
Dental Pulp ◽  
Tooth Development ◽  
Dental Pulp Cells ◽  
Pulp Cells ◽  
Two Populations

Altered gene expression in human cleidocranial dysplasia dental pulp cells

2005 ◽  
Vol 50 (2) ◽  
pp. 227-236 ◽  
Author(s):  
Shuo Chen ◽  
Lori Santos ◽  
Yimin Wu ◽  
Rose Vuong ◽  
Isabel Gay ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dental Pulp ◽  
Cleidocranial Dysplasia ◽  
Dental Pulp Cells ◽  
Altered Gene Expression ◽  
Pulp Cells ◽  
Altered Gene

Abnormal Differentiation of Dental Pulp Cells in Cleidocranial Dysplasia

2015 ◽  
Vol 94 (4) ◽  
pp. 577-583 ◽  
Author(s):  
W.J. Yan ◽  
C.Y. Zhang ◽  
X. Yang ◽  
Z.N. Liu ◽  
X.Z. Wang ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Cleidocranial Dysplasia ◽  
Dental Pulp Cells ◽  
Pulp Cells

scholarly journals Runx3 negatively regulates Osterix expression in dental pulp cells

2007 ◽  
Vol 405 (1) ◽  
pp. 69-75 ◽  
Author(s):  
Li Zheng ◽  
Koichiro Iohara ◽  
Masaki Ishikawa ◽  
Takeshi Into ◽  
Teruko Takano-Yamamoto ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Dental Pulp ◽  
Tooth Development ◽  
Tooth Germ ◽  
Dental Pulp Cells ◽  
Mobility Shift ◽  
Pulp Cells ◽  
Mobility Shift Assay ◽  
Responsive Element

Osterix, a zinc-finger-containing transcription factor, is required for osteoblast differentiation and bone formation. Osterix is also expressed in dental mesenchymal cells of the tooth germ. However, transcriptional regulation by Osterix in tooth development is not clear. Genetic studies in osteogenesis place Osterix downstream of Runx2 (Runt-related 2). The expression of Osterix in odontoblasts overlaps with Runx3 during terminal differentiation in vivo. Runx3 down-regulates Osterix expression in mouse DPCs (dental pulp cells). Therefore the regulatory role of Runx3 on Osterix expression in tooth development was investigated. Enforced expression of Runx3 down-regulated the activity of the Osterix promoter in the human embryonic kidney 293 cell line. When the Runx3 responsive element on the Osterix promoter, located at −713 to −707 bp (site 3, AGTGGTT) relative to the cap site, was mutated, this down-regulation was abrogated. Furthermore, electrophoretic mobility-shift assay and chromatin immunoprecipitation assays in mouse DPCs demonstrated direct functional binding of Runx3 to the Osterix promoter. These results demonstrate the transcriptional regulation of Osterix expression by Runx3 during differentiation of dental pulp cells into odontoblasts during tooth development.

scholarly journals The Preparation and Characterization of Chitosan-Based Hydrogels Cross-Linked by Glyoxal

Materials ◽  
2021 ◽  
Vol 14 (9) ◽  
pp. 2449
Author(s):  
Beata Kaczmarek-Szczepańska ◽  
Olha Mazur ◽  
Marta Michalska-Sionkowska ◽  
Krzysztof Łukowicz ◽  
Anna Maria Osyczka
Keyword(s):  
Thermal Stability ◽  
Tannic Acid ◽  
Dental Pulp ◽  
Blood Compatibility ◽  
Dental Pulp Cells ◽  
Preliminary Assessment ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells

In this study, hydrogels based on chitosan cross-linked by glyoxal have been investigated for potential medical applications. Hydrogels were loaded with tannic acid at different concentrations. The thermal stability and the polyphenol-releasing rate were determined. For a preliminary assessment of the clinical usefulness of the hydrogels, they were examined for blood compatibility and in the culture of human dental pulp cells (hDPC). The results showed that after immersion in a polyphenol solution, chitosan/glyoxal hydrogels remain nonhemolytic for erythrocytes, and we also did not observe the cytotoxic effect of hydrogels immersed in tannic acid (TA) solutions with different concentration. Tannic acid was successfully released from hydrogels, and its addition improved material thermal stability. Thus, the current findings open the possibility to consider such hydrogels in clinics.

scholarly journals Cytotoxicity assessment of different doses of ozonated water on dental pulp cells

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Ferdiye Küçük ◽  
Sibel Yıldırım ◽  
Serap Çetiner
Keyword(s):  
Cell Viability ◽  
Repeated Measures ◽  
Dental Pulp ◽  
Control Group ◽  
Dental Pulp Cells ◽  
Time Points ◽  
Significant Difference ◽  
Pulp Cells ◽  
Time Point ◽  
Ozonated Water

Abstract Background The purpose of this study was to assess the cytotoxicity of various concentrations of ozonated water (OW) on human primary dental pulp cells. Methods Human primary dental pulp cells were isolated from exfoliated primary canine teeth of an 11-year-old patient with good systemic and oral health. Afterwards, cells were divided into 6 experimental groups; four groups of OW in concentrations of 2 mg/L, 4 mg/L, 8 mg/L, and 16 mg/L, untreated control group, and cell culture without cells. Cytotoxicity was evaluated after exposure for 5-min exposure using Mosmann’s Tetrazolium Toxicity (MTT) assay at 0 h and 48 h time points. Data were analyzed using a repeated measures analysis of variance and Post-hoc tests were performed using Bonferroni correction for multiple comparisons. Results All experimental groups showed proliferation at 0 h time point. However, all groups also experienced a decrease in overtime at 48 h time point (p < 0.05). At both time points 2 mg/L OW showed the highest cell viability as well as proliferation. At 0 h time point, the increase in cell viability for all experimental groups was found statistically significant when compared to positive control group (p < 0.05). At 48 h time point, although 8 mg/L and 16 mg/L OW showed statistically significant reduction in compare to 0 h time point, 2 mg/L and 4 mg/L OW groups didn’t experience any statistically significant difference (p < 0.05). Conclusion Considering our findings, due to ozonated water's induced a higher proliferation rate of dental pulp cells, indicating their biocompatibility and a possible adjuvant on irrigating agent in regenerative endodontic procedures.

The molecular functions of Biodentine and MTA in LPS‐induced inflamed dental pulp cells

2021 ◽  
Author(s):  
M.C. Wang ◽  
H.F. Tu ◽  
K.W. Chang ◽  
S.C. Lin ◽  
L.Y. Yeh ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Pulp Cells ◽  
Molecular Functions

HGF Enhanced Proliferation and Differentiation of Dental Pulp Cells

2006 ◽  
Vol 32 (8) ◽  
pp. 736-741 ◽  
Author(s):  
Ling Ye ◽  
Li Peng ◽  
Hong Tan ◽  
Xuedong Zhou
Keyword(s):  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Proliferation And Differentiation ◽  
Pulp Cells
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