scholarly journals Multilineage differentiation potential of cells isolated from the human amniotic membrane

2010 ◽  
Vol 111 (4) ◽  
pp. 846-857 ◽  
Author(s):  
Silvia Díaz-Prado ◽  
Emma Muiños-López ◽  
Tamara Hermida-Gómez ◽  
Maria Esther Rendal-Vázquez ◽  
Isaac Fuentes-Boquete ◽  
...  
Keyword(s):  
Amniotic Membrane ◽  
Human Amniotic Membrane ◽  
Multilineage Differentiation ◽  
Differentiation Potential

scholarly journals The Osteogenic Capacity of HumanAmniotic Membrane Mesenchymal Stem Cell (hAMSC) and Potential for Application in Maxillofacial Bone Reconstruction in Vitro Study

2020 ◽  
Vol 4 (1) ◽  
pp. 17
Author(s):  
David Kamadjaja
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Osteogenic Differentiation ◽  
Amniotic Membrane ◽  
Isolation Procedure ◽  
Target Cells ◽  
Human Amniotic Membrane ◽  
Bone Reconstruction ◽  
Differentiation Potential

Amniotic membrane of human placenta is a source of abundant mesenchymal stem cell (hAMSC) which makes it a potential source of allogeneic multipotent cell for bone healing.  However, much has to be explored about its isolation procedure and the osteogenic differentiation potential. The aims of this study are to establish the procurement procedure of human amniotic membrane, the isolation   and culture   of hAMSC, the MSC phenotypic characterization, and the in vitro osteogenic differentiation of hAMSC.  Results of the study are as follows. The quality of human amniotic membrane would be best if procured from Caesarean operation under highly aseptic condition to avoid fungal and bacterial contamination on the culture.  Isolation procedure using modified   Soncini protocol yielded large amount of MSC with high proliferative capacity in culture medium.  Characterization of hAMSC showed that the majority of the target cells exhibited specific MSC markers (CD10S and CD90) with a small number of these cells expressing CD45the marker of hematopoeitic cells. The in vitro osteogenic differentiation of hAMSC  followed by Alizarin  Red staining showed that  osteoblastic differentiation  was  detected in a significantly   high  number  of cells.  This study concludes that hAMSCs isolated from human amniotic membrane have the capacity for in vitro osteogenesis which makes them be one of the potential allogeneic stem cells for application in maxillofacial bone reconstruction.

scholarly journals Sub-Regional Differences of the Human Amniotic Membrane and Their Potential Impact on Tissue Regeneration Application

2021 ◽  
Vol 8 ◽  
Author(s):  
Adelheid Weidinger ◽  
Laura Poženel ◽  
Susanne Wolbank ◽  
Asmita Banerjee
Keyword(s):  
Tissue Regeneration ◽  
Amniotic Membrane ◽  
Human Amniotic Membrane ◽  
Anatomical Location ◽  
Differentiation Potential ◽  
Proliferation And Differentiation ◽  
Reprogramming Efficiency ◽  
Potential Impact ◽  
Structural Entity ◽  
Fine Tune

For more than 100 years, the human amniotic membrane (hAM) has been used in multiple tissue regeneration applications. The hAM consists of cells with stem cell characteristics and a rich layer of extracellular matrix. Undoubtedly, the hAM with viable cells has remarkable properties such as the differentiation potential into all three germ layers, immuno-modulatory, and anti-fibrotic properties. At first sight, the hAM seems to be one structural entity. However, by integrating its anatomical location, the hAM can be divided into placental, reflected, and umbilical amniotic membrane. Recent studies show that cells of these amniotic sub-regions differ considerably in their properties such as morphology, structure, and content/release of certain bioactive factors. The aim of this review is to summarize these findings and discuss the relevance of these different properties for tissue regeneration. In summary, reflected amnion seems to be more immuno-modulatory and could have a higher reprogramming efficiency, whereas placental amnion seems to be pro-inflammatory, pro-angiogenic, with higher proliferation and differentiation capacity (e.g., chondrogenic and osteogenic), and could be more suitable for certain graft constructions. Therefore, we suggest that the respective hAM sub-region should be selected in consideration of its desired outcome. This will help to optimize and fine-tune the clinical application of the hAM.

scholarly journals Can Human Oral Mucosa Stem Cells Differentiate to Corneal Epithelia?

2021 ◽  
Vol 22 (11) ◽  
pp. 5976
Author(s):  
Sonia López ◽  
Lía Hoz ◽  
Eda Patricia Tenorio ◽  
Beatriz Buentello ◽  
Fátima Sofía Magaña ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Oral Mucosa ◽  
Amniotic Membrane ◽  
Embryonic Stem ◽  
Human Amniotic Membrane ◽  
Anatomical Location ◽  
Multilineage Differentiation ◽  
Therapeutic Alternative ◽  
E Cadherin

Human oral mucosa stem cells (hOMSCs) arise from the neural crest, they can self-renew, proliferate, and differentiate to several cell lines and could represent a good source for application in tissue engineering. Because of their anatomical location, hOMSCs are easy to isolate, have multilineage differentiation capacity and express embryonic stem cells markers such as—Sox2, Oct3/4 and Nanog. We have used SHEM (supplemented hormonal epithelial medium) media and cultured hOMSCs over human amniotic membrane and determined the cell’s capacity to differentiate to an epithelial-like phenotype and to express corneal specific epithelial markers—CK3, CK12, CK19, Pan-cadherin and E-cadherin. Our results showed that hOMSCs possess the capacity to attach to the amniotic membrane and express CK3, CK19, Pan-Cadherin and E-Cadherin without induction with SHEM media and expressed CK12 or changed the expression pattern of E-Cadherin to a punctual-like feature when treated with SHEM media. The results observed in this study show that hOMSCs possess the potential to differentiate toward epithelial cells. In conclusion, our results revealed that hOMSCs readily express markers for corneal determination and could provide the ophthalmology field with a therapeutic alternative for tissue engineering to achieve corneal replacement when compared with other techniques. Nevertheless, further studies are needed to develop a predictable therapeutic alternative for cornea replacement.

Effect of viable cryopreserved human amniotic membrane sutured to cornea on course of experimental bacterial keratitis

2015 ◽  
Vol 57 (5) ◽  
pp. 35-41
Author(s):  
K. Sereda ◽  
◽  
G. Drozhzhina ◽  
T. Gaidamaka ◽  
V. Vit ◽  
...  
Keyword(s):  
Amniotic Membrane ◽  
Human Amniotic Membrane ◽  
Bacterial Keratitis

scholarly journals Human Amniotic Membrane as a Matrix for Endothelial Differentiation of VEGF-Treated Dental Stem Cells

2019 ◽  
Vol 12 (6) ◽  
pp. 599-613 ◽  
Author(s):  
Siti Nurnasihah Md Hashim ◽  
Muhammad Fuad Hilmi Yusof ◽  
Wafa’ Zahari ◽  
Hamshawagini Chandra ◽  
Khairul Bariah Ahmad Amin Noordin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Amniotic Membrane ◽  
Endothelial Differentiation ◽  
Human Amniotic Membrane ◽  
Dental Stem Cells

Human amniotic membrane as a delivery vehicle for stem cell-based therapies

Life Sciences ◽  
2021 ◽  
pp. 119157
Author(s):  
Ping Chen ◽  
Minjun Lu ◽  
Tao Wang ◽  
Dongchun Dian ◽  
Yong Zhong ◽  
...  
Keyword(s):  
Stem Cell ◽  
Amniotic Membrane ◽  
Human Amniotic Membrane ◽  
Delivery Vehicle
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