Rottlerin inhibits migration of follicular thyroid carcinoma cells by PKCδ-independent destabilization of the focal adhesion complex

2010 ◽  
pp. n/a-n/a ◽  
Author(s):  
Chien-Jung Lin ◽  
Chieh-Yu Lin ◽  
Ying Chen ◽  
Shih-Horng Huang ◽  
Seu-Mei Wang
Keyword(s):  
Thyroid Carcinoma ◽  
Focal Adhesion ◽  
Follicular Thyroid Carcinoma ◽  
Carcinoma Cells ◽  
Focal Adhesion Complex ◽  
Adhesion Complex

Increased transcriptional response to mechanical strain in keloid fibroblasts due to increased focal adhesion complex formation

2005 ◽  
Vol 206 (2) ◽  
pp. 510-517 ◽  
Author(s):  
Zhen Wang ◽  
Kenton D. Fong ◽  
Toan-Thang Phan ◽  
Ivor J. Lim ◽  
Michael T. Longaker ◽  
...  
Keyword(s):  
Complex Formation ◽  
Focal Adhesion ◽  
Mechanical Strain ◽  
Transcriptional Response ◽  
Focal Adhesion Complex ◽  
Adhesion Complex ◽  
Keloid Fibroblasts

scholarly journals PHIP drives glioblastoma motility and invasion by regulating the focal adhesion complex

2020 ◽  
Vol 117 (16) ◽  
pp. 9064-9073
Author(s):  
David de Semir ◽  
Vladimir Bezrookove ◽  
Mehdi Nosrati ◽  
Kara R. Scanlon ◽  
Eric Singer ◽  
...  
Keyword(s):  
Focal Adhesion ◽  
Pleckstrin Homology Domain ◽  
Physical Interaction ◽  
Glioblastoma Cells ◽  
Focal Adhesion Complex ◽  
Adhesion Proteins ◽  
Focal Adhesion Proteins ◽  
Tumor Cell Motility ◽  
Adhesion Complex ◽  
Force Transduction

The invasive behavior of glioblastoma is essential to its aggressive potential. Here, we show that pleckstrin homology domain interacting protein (PHIP), acting through effects on the force transduction layer of the focal adhesion complex, drives glioblastoma motility and invasion. Immunofluorescence analysis localized PHIP to the leading edge of glioblastoma cells, together with several focal adhesion proteins: vinculin (VCL), talin 1 (TLN1), integrin beta 1 (ITGB1), as well as phosphorylated forms of paxillin (pPXN) and focal adhesion kinase (pFAK). Confocal microscopy specifically localized PHIP to the force transduction layer, together with TLN1 and VCL. Immunoprecipitation revealed a physical interaction between PHIP and VCL. Targeted suppression of PHIP resulted in significant down-regulation of these focal adhesion proteins, along with zyxin (ZYX), and produced profoundly disorganized stress fibers. Live-cell imaging of glioblastoma cells overexpressing a ZYX-GFP construct demonstrated a role for PHIP in regulating focal adhesion dynamics. PHIP silencing significantly suppressed the migratory and invasive capacity of glioblastoma cells, partially restored following TLN1 or ZYX cDNA overexpression. PHIP knockdown produced substantial suppression of tumor growth upon intracranial implantation, as well as significantly reduced microvessel density and secreted VEGF levels. PHIP copy number was elevated in the classical glioblastoma subtype and correlated with elevated EGFR levels. These results demonstrate PHIP’s role in regulating the actin cytoskeleton, focal adhesion dynamics, and tumor cell motility, and identify PHIP as a key driver of glioblastoma migration and invasion.

scholarly journals Molecular Signature of Prospero Homeobox 1 (PROX1) in Follicular Thyroid Carcinoma Cells

2019 ◽  
Vol 20 (9) ◽  
pp. 2212 ◽  
Author(s):  
Magdalena Rudzińska ◽  
Małgorzata Grzanka ◽  
Anna Stachurska ◽  
Michał Mikula ◽  
Katarzyna Paczkowska ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Thyroid Carcinoma ◽  
Follicular Thyroid Carcinoma ◽  
Carcinoma Cells ◽  
Molecular Signature ◽  
Transcriptional Analysis ◽  
Rna Seq ◽  
Cytoskeleton Organization ◽  
Transcriptional Signature ◽  
Interfering Rna

The prospero homeobox 1 (PROX1) transcription factor is a product of one of the lymphangiogenesis master genes. It has also been suggested to play a role in carcinogenesis, although its precise role in tumour development and metastasis remains unclear. The aim of this study was to gain more knowledge on the PROX1 function in thyroid tumorigenesis. Follicular thyroid cancer-derived cells—CGTH-W-1—were transfected with PROX1-siRNA (small interfering RNA) and their proliferation, cell cycle, apoptosis and motility were then analysed. The transcriptional signature of PROX1 depletion was determined using RNA-Sequencing (RNA-Seq) and the expression of relevant genes was further validated using reverse transcriptase quantitative PCR (RT-qPCR), Western blot and immunocytochemistry. PROX1 depletion resulted in a decreased cell motility, with both migratory and invasive potential being significantly reduced. The cell morphology was also affected, while the other studied cancer-related cell characteristics were not significantly altered. RNA-seq analysis revealed significant changes in the expression of transcripts encoding genes involved in both motility and cytoskeleton organization. Our transcriptional analysis of PROX1-depleted follicular thyroid carcinoma cells followed by functional and phenotypical analyses provide, for the first time, evidence that PROX1 plays an important role in the metastasis of thyroid cancer cells by regulating genes involved in focal adhesion and cytoskeleton organization in tumour cells.

Simulated microgravity alters differentiation and increases apoptosis in human follicular thyroid carcinoma cells

2002 ◽  
Vol 16 (6) ◽  
pp. 604-606 ◽  
Author(s):  
Daniela Grimm ◽  
Johann Bauer ◽  
Peter Kossmehl ◽  
Mehdi Shakibaei ◽  
Johann Schönberger ◽  
...  
Keyword(s):  
Thyroid Carcinoma ◽  
Simulated Microgravity ◽  
Follicular Thyroid Carcinoma ◽  
Carcinoma Cells

scholarly journals The role of prospero homeobox 1 (PROX1) expression in follicular thyroid carcinoma cells

Oncotarget ◽  
2017 ◽  
Vol 8 (69) ◽  
pp. 114136-114155 ◽  
Author(s):  
Magdalena Rudzinska ◽  
Joanna K. Ledwon ◽  
Damian Gawel ◽  
Justyna Sikorska ◽  
Barbara Czarnocka
Keyword(s):  
Thyroid Carcinoma ◽  
Follicular Thyroid Carcinoma ◽  
Carcinoma Cells ◽  
Prox1 Expression

Beta3-integrin–mediated focal adhesion complex formation: adult cardiocytes embedded in three-dimensional polymer matrices

1999 ◽  
Vol 83 (12) ◽  
pp. 38-43 ◽  
Author(s):  
Toshio Nagai ◽  
Martin Laser ◽  
Catalin F Baicu ◽  
Michael R Zile ◽  
George Cooper ◽  
...  
Keyword(s):  
Complex Formation ◽  
Focal Adhesion ◽  
Three Dimensional ◽  
Polymer Matrices ◽  
Focal Adhesion Complex ◽  
Beta3 Integrin ◽  
Adhesion Complex
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