Atrial natriuretic peptide attenuates high glucose-activated transforming growth factor-β, Smad and collagen synthesis in renal proximal tubular cells

2008 ◽  
Vol 103 (6) ◽  
pp. 1999-2009 ◽  
Author(s):  
Chao-Sheng Lo ◽  
Zhao-Hong Chen ◽  
Tusty-Jiuan Hsieh ◽  
Shyi-Jang Shin
Keyword(s):  
Growth Factor ◽  
Atrial Natriuretic Peptide ◽  
Collagen Synthesis ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β ◽  
Proximal Tubular Cells ◽  
Tubular Cells ◽  
Proximal Tubular ◽  
Renal Proximal Tubular Cells ◽  
Renal Proximal Tubular

scholarly journals Essential Role(s) of the Intrarenal Renin-Angiotensin System in Transforming Growth Factor–β1 Gene Expression and Induction of Hypertrophy of Rat Kidney Proximal Tubular Cells in High Glucose

2002 ◽  
Vol 13 (2) ◽  
pp. 302-312
Author(s):  
Shao-Ling Zhang ◽  
Catherine To ◽  
Xing Chen ◽  
Janos G. Filep ◽  
Shiow-Shih Tang ◽  
...  
Keyword(s):  
Gene Expression ◽  
High Glucose ◽  
Transforming Growth Factor ◽  
Proximal Tubular Cells ◽  
Tubular Cells ◽  
Type Iv ◽  
Proximal Tubular ◽  
Renal Proximal Tubular Cells ◽  
Renal Proximal Tubular ◽  
Tgf Β1

ABSTRACT. These studies investigated the question of whether the intrarenal renin-angiotensin system (RAS) is essential for transforming growth factor–β1 (TGF-β1) gene expression and induction of hypertrophy of renal proximal tubular cells in high glucose in vitro. Antisense and sense angiotensinogen (ANG) cDNAs were stably transfected into rat immortalized renal proximal tubular cells (IRPTC). ANG secretion from rat IRPTC was quantified by a specific RIA for rat ANG. Cellular ANG, TGF-β1, and collagen α1 (type IV) mRNA levels were determined by Northern blot analysis or by reverse transcriptase–PCR assay. Hypertrophy of IRPTC was analyzed by Western blotting of cellular p27Kip1 protein, flow cytometry, and cellular protein assay. The results showed that stable transfer of antisense ANG cDNA into IRPTC suppressed the basal TGF-β1 and collagen α1 (type IV) mRNA expression and blocked the stimulatory effect of high glucose (i.e., 25 mM) on TGF-β1 and collagen α1 (type IV) mRNA expression and induction of IRPTC hypertrophy. In contrast, stable transfer of sense ANG cDNA into IRPTC had no significant effect on these parameters. These data demonstrate that local intrarenal RAS activation is essential for TGF-β1 gene expression and induction of hypertrophy of renal proximal tubular cells in high glucose.

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