Purification and growth of endothelial progenitor cells from murine bone marrow mononuclear cells

2007 ◽  
Vol 103 (1) ◽  
pp. 21-29 ◽  
Author(s):  
Qi Ru Wang ◽  
Bao He Wang ◽  
Yan Hong Huang ◽  
Guo Dai ◽  
Wei Ming Li ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Mononuclear Cells ◽  
Bone Marrow Mononuclear Cells ◽  
Endothelial Progenitor ◽  
Murine Bone Marrow

scholarly journals Improved Culture-Based Isolation of Differentiating Endothelial Progenitor Cells from Mouse Bone Marrow Mononuclear Cells

PLoS ONE ◽  
2011 ◽  
Vol 6 (12) ◽  
pp. e28639 ◽  
Author(s):  
Haruki Sekiguchi ◽  
Masaaki Ii ◽  
Kentaro Jujo ◽  
Ayumi Yokoyama ◽  
Nobuhisa Hagiwara ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Mononuclear Cells ◽  
Mouse Bone Marrow ◽  
Bone Marrow Mononuclear Cells ◽  
Endothelial Progenitor

scholarly journals CD34+ Cells Represent Highly Functional Endothelial Progenitor Cells in Murine Bone Marrow

PLoS ONE ◽  
2011 ◽  
Vol 6 (5) ◽  
pp. e20219 ◽  
Author(s):  
Junjie Yang ◽  
Masaaki Ii ◽  
Naosuke Kamei ◽  
Cantas Alev ◽  
Sang-Mo Kwon ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Endothelial Progenitor ◽  
Murine Bone Marrow ◽  
Cd34 Cells

scholarly journals Reduced Ischemic Brain Injury by Partial Rejuvenation of Bone Marrow Cells in Aged Rats

2010 ◽  
Vol 31 (3) ◽  
pp. 855-867 ◽  
Author(s):  
Akihiko Taguchi ◽  
Pengxiang Zhu ◽  
Fang Cao ◽  
Akie Kikuchi-Taura ◽  
Yukiko Kasahara ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Mononuclear Cells ◽  
Bone Marrow Cells ◽  
Aged Rats ◽  
Endothelial Progenitor ◽  
Ischemic Brain ◽  
Marrow Cells

Circulating bone marrow-derived immature cells, including endothelial progenitor cells, have been implicated in homeostasis of the microvasculature. Decreased levels of circulating endothelial progenitor cells, associated with aging and/or cardiovascular risk factors, correlate with poor clinical outcomes in a range of cardiovascular diseases. Herein, we transplanted bone marrow cells from young stroke-prone spontaneously hypertensive rats (SHR-SP) into aged SHR-SP, the latter not exposed to radiation or chemotherapy. Analysis of recipient peripheral blood 28 days after transplantation revealed that 5% of circulating blood cells were of donor origin. Cerebral infarction was induced on day 30 posttransplantation. Animals transplanted with bone marrow from young SHR-SP displayed an increase in density of the microvasculature in the periinfarction zone, reduced ischemic brain damage and improved neurologic function. In vitro analysis revealed enhanced activation of endothelial nitric oxide synthase and reduced activation p38 microtubule-associated protein (MAP) kinase, the latter associated with endothelial apoptosis, in cultures exposed to bone marrow-derived mononuclear cells from young animals versus cells from aged counterparts. Our findings indicate that partial rejuvenation of bone marrow from aged rats with cells from young animals enhances the response to ischemic injury, potentially at the level of endothelial/vascular activation, providing insight into a novel approach ameliorate chronic vascular diseases.

Endothelial Progenitor Cells for Vasculogenesis

Physiology ◽  
2005 ◽  
Vol 20 (1) ◽  
pp. 36-42 ◽  
Author(s):  
Satoshi Murasawa ◽  
Takayuki Asahara
Keyword(s):  
Bone Marrow ◽  
Endothelial Cells ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Mononuclear Cells ◽  
Attractive Potential ◽  
Therapeutic Application ◽  
Endothelial Progenitor ◽  
Potential Therapeutic Application ◽  
Ischemic Diseases

Postnatal vasculogenesis is considered to be involved in neovascularization of adult tissues, because bone marrow-derived endothelial progenitor cells (EPCs) were isolated from circulating mononuclear cells in peripheral blood and were shown to incorporate into sites of physiological and pathological neovascularization and to differentiate into mature endothelial cells. EPCs might have an attractive potential therapeutic application for cardiovascular ischemic diseases as a novel cell-based strategy mainly via a vasculogenesis mechanism.

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