Expression of cyclin D3 through Sp1 sites by histone deacetylase inhibitors is mediated with protein kinase C-δ (PKC-δ) signal pathway

2007 ◽  
Vol 101 (4) ◽  
pp. 987-995 ◽  
Author(s):  
Young-Ho Kim ◽  
Jun Hee Lim ◽  
Tae-Jin Lee ◽  
Jong-Wook Park ◽  
Taeg Kyu Kwon
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Histone Deacetylase ◽  
Histone Deacetylase Inhibitors ◽  
Signal Pathway ◽  
Cyclin D3 ◽  
Kinase C

scholarly journals Identification of Combinations of Protein Kinase C Activators and Histone Deacetylase Inhibitors that Potently Reactivate Latent HIV

Viruses ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 609
Author(s):  
Francesca Curreli ◽  
Shahad Ahmed ◽  
Sofia M. Benedict Victor ◽  
Asim K. Debnath
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Synergistic Effect ◽  
Histone Deacetylase ◽  
Histone Deacetylase Inhibitors ◽  
Latent Virus ◽  
Hiv Latency ◽  
Kinase C ◽  
Protein Kinase C Activators ◽  
Latent Hiv

Combination antiretroviral therapy (cART) is successful in maintaining undetectable levels of HIV in the blood; however, the persistence of latent HIV reservoirs has become the major barrier for a HIV cure. Substantial efforts are underway in finding the best latency-reversing agents (LRAs) to purge the latent viruses from the reservoirs. We hypothesize that identifying the right combination of LRAs will be the key to accomplishing that goal. In this study, we evaluated the effect of combinations of three protein kinase C activators (prostratin, (-)-indolactam V, and TPPB) with four histone deacetylase inhibitors (AR-42, PCI-24781, givinostat, and belinostat) on reversing HIV latency in different cell lines including in a primary CD4+ T-cell model. Combinations including indolactam and TPPB with AR-42 and PCI produced a strong synergistic effect in reactivating latent virus as indicated by higher p24 production and envelope gp120 expression. Furthermore, treatment with TPPB and indolactam greatly downregulated the cellular receptor CD4. Indolactam/AR-42 combination emerged from this study as the best combination that showed a strong synergistic effect in reactivating latent virus. Although AR-42 alone did not downregulate CD4 expression, indolactam/AR-42 showed the most efficient downregulation. Our results suggest that indolactam/AR-42 is the most effective combination, showing a strong synergistic effect in reversing HIV latency combined with the most efficient CD4 downregulation.

Protein kinase C-alpha antagonizes apoptosis induction by histone deacetylase inhibitors in multidrug resistant leukaemia cells

2007 ◽  
Vol 39 (10) ◽  
pp. 1877-1885 ◽  
Author(s):  
María D. Castro-Galache ◽  
Maria P. Menéndez-Gutiérrez ◽  
Estefania Carrasco Garcia ◽  
Pilar Garcia-Morales ◽  
Isabel Martinez-Lacaci ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Histone Deacetylase ◽  
Histone Deacetylase Inhibitors ◽  
Multidrug Resistant ◽  
Apoptosis Induction ◽  
Kinase C ◽  
Protein Kinase C Alpha

scholarly journals Phospholipase C beta1 (PI‐PLCbeta1)/Cyclin D3/protein kinase C (PKC) alpha signaling modulation during iron‐induced oxidative stress in myelodysplastic syndromes (MDS)

2020 ◽  
Vol 34 (11) ◽  
pp. 15400-15416
Author(s):  
Alessandra Cappellini ◽  
Sara Mongiorgi ◽  
Carlo Finelli ◽  
Antonietta Fazio ◽  
Stefano Ratti ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Phospholipase C ◽  
Myelodysplastic Syndromes ◽  
Cyclin D3 ◽  
Kinase C ◽  
Pkc Alpha

scholarly journals Sphingolipid synthesis and role in uterine epithelia proliferation

Reproduction ◽  
2018 ◽  
Vol 156 (2) ◽  
pp. 173-183 ◽  
Author(s):  
Jorge Cerbón ◽  
Noemi Baranda-Avila ◽  
Alejandro Falcón-Muñoz ◽  
Ignacio Camacho-Arroyo ◽  
Marco Cerbón
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Epithelial Cells ◽  
Estrous Cycle ◽  
Cyclin D3 ◽  
Uterine Epithelial Cells ◽  
Uterine Epithelial Cell ◽  
Kinase C

Sphingolipids are involved in the regulation of cell proliferation. It has been reported that diacylglycerol and sphingosine-1-phosphate generation, during the synthesis of phospho-sphingolipids, is necessary for both, G1-S transition of cell cycle during the sustained activation of protein kinase C in various cell models (MDCK,SaccharomycesandEntamoeba) and AKT pathway activation. During the estrous cycle of the rat, AKT signaling is the main pathway involved in the regulation of uterine cell proliferation. The aim of the present study was to investigate the role of sphingolipid synthesis during proliferation of uterine cells in the estrous cycle of the rat. On metestrus day, when both luminal and glandular uterine epithelia present the maximal BrdU-labeled cells (S phase cells), there was an increase in the relative abundance of total sphingomyelins, as compared to estrus day. Myriocin, a sphingolipid synthesis inhibitor administered on estrus day, before the new cell cycle of epithelial cells is initiated, decreased the abundance of sphingomyelin, accompanied by proliferation arrest in uterine epithelial cells on metestrus day. In order to study the sphingolipid signaling pathway affected by myriocin, we evaluated the activation of the PKC-AKT-GSK3b-Cyclin D3 pathway. We observed that total and phosphorylated protein kinase C diminished in uterine epithelial cells of myriocin treated animals. Interestingly, cyclin D3 nuclear localization was blocked by myriocin, concomitantly with a decrease in nuclear pRb expression. In conclusion, we demonstrate that sphingolipid synthesis and signaling are involved in uterine epithelial cell proliferation during the estrous cycle of the rat.

scholarly journals Epidermal growth factor receptor activation by protein kinase C is necessary for FSH-induced meiotic resumption in porcine cumulus–oocyte complexes

2008 ◽  
Vol 197 (2) ◽  
pp. 409-419 ◽  
Author(s):  
Xiufen Chen ◽  
Bo Zhou ◽  
Jun Yan ◽  
Baoshan Xu ◽  
Ping Tai ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Growth Factor ◽  
Protein Kinase ◽  
Cumulus Cells ◽  
Inhibitory Effect ◽  
Signal Pathway ◽  
Kinase C ◽  
Egfr Activation ◽  
Porcine Oocyte ◽  
Epidermal Growth

It is proved that epidermal growth factor (EGF)-like factors mediate gonadotropin-induced rodent oocyte maturation via EGF receptor (EGFR). However, the detail kinetics and signal pathway between FSH and EGF/EGFR is not clear in large animals. In the present study, we investigated the roles of EGFR and protein kinase C (PKC) in FSH-induced porcine oocyte meiotic resumption. Porcine cumulus–oocyte complexes were cultured in NCSU37 medium containing 10% porcine follicular fluid and germinal vesicle breakdown (meiotic resumption) was detected after different treatments. The results showed that EGF-like factor amphiregulin (AR) and EGFR mRNA were expressed in porcine cumulus cells, but not oocytes. FSH significantly induced AR mRNA expression with maximum at 4 h and activated EGFR phosphorylation at 8 h. AR (1–100 ng/ml) dose-dependently induced meiosis resumption of porcine oocyte. The specific EGFR inhibitor, AG1478, but not AG43 (the inactive analog of AG1478), completely blocked FSH, EGF, and AR-induced oocyte meiotic resumption; the inhibitory effect of AG1478 on FSH action gradually decreased when the inhibitor was added at 6 h or later and disappeared when it was added at 11 h; EGF reversed the inhibitory effect on FSH when AG1478 was added within 6 h. FSH triggered porcine oocyte meiotic resumption (at 20 h) later than that of EGF and AR (at 18 h). All these results supported that endogenously produced EGFR activator(s), possibly AR (maximum at 4 h) and EGFR activation (began at 6 h and finished within 11 h), in cumulus cells is necessary for FSH-induced porcine oocyte meiotic resumption (began at 18 h). Furthermore, PKC activator PMA mimicked but PKC inhibitor chelerythrine chloride inhibited FSH action, and AG1478 also suppressed PMA-induced porcine oocyte meiotic resumption. These data together suggested that EGFR activation, by PKC signal pathway, participates in FSH-induced porcine oocyte meiotic resumption.

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