scholarly journals Under-investigated area in prostate cancer: cross talk between the bone microenvironment and prostate cancer bone metastasis

2004 ◽  
Vol 91 (4) ◽  
pp. 684-685 ◽  
Author(s):  
Suresh Mohla
Keyword(s):  
Prostate Cancer ◽  
Bone Metastasis ◽  
Cross Talk ◽  
Bone Microenvironment

scholarly journals A Novel in vitro Model for Prostate Cancer Bone Metastasis using Microfluidics

2020 ◽  
Vol 1 (2) ◽  
Author(s):  
Alexander Cui ◽  
Et al.
Keyword(s):  
Prostate Cancer ◽  
Bone Metastasis ◽  
Rna Sequencing ◽  
Cell Types ◽  
Pcr Analysis ◽  
Bone Microenvironment ◽  
Sequencing Library ◽  
Multiple Cell ◽  
Vitro Model

Alexander Cui, Srinivasa Rao, Sam Olechnowicz, James Edwards, Peter Cook, Edmond Walsh, Claire Edwards Prostate cancer (PCa) cells predominantly metastasise to bone, and once this happens the disease is incurable. Hence it is vital to understand the mechanism of bone metastasis in PCa. Several models have been established to study PCa bone metastasis in vitro, involving the co-culture of PCa cell lines with cells of the bone microenvironment such as osteoblasts, osteoclasts, bone marrow stromal cells, etc. However, these models are limited to one-to-one co-culture (i.e., PCa cells co-cultured with one other cell type), which overly simplifies the complex bone microenvironment consisting of multiple cell types. We aimed to overcome this limitation by adapting a microfluidics platform that employs a novel mechanism to isolate sub-microliter volumes using fluid walls. By using this technology, we were able to successfully differentiate osteoblasts and adipocytes from precursor cells (2T3 and ST2 cells respectively) in nanowells, and co-cultured them with PC3 prostate cancer cells. Differentiation to osteoblasts and adipocytes was confirmed by calcein green and BODIPY live staining which could be monitored using the Incucyte imaging platform. Following co-culture, the PCa cells were trypsinised and total RNA was extracted from them. A cDNA amplification step was successfully incorporated into the reverse transcription protocol to provide sufficient material for quantitative PCR analysis. Further, sequencing adapters were added to the cDNA to generate an RNA-sequencing library for transcriptome-wide analysis. Using this novel model, with imaging, RNA-sequencing and qPCR as read-outs, we are working on elucidating the signalling pathways that are activated by the bone microenvironment in PCa cells. 

scholarly journals Hybrid Discrete-Continuum Cellular Automaton (HCA) model of Prostate to Bone Metastasis

2016 ◽  
Author(s):  
Arturo Araujo ◽  
David Basanta
Keyword(s):  
Prostate Cancer ◽  
Bone Metastasis ◽  
Molecular Mechanisms ◽  
Bone Matrix ◽  
Cellular Interactions ◽  
Vicious Cycle ◽  
Bone Microenvironment ◽  
Basic Multicellular Unit ◽  
Bone Degradation ◽  
The Impact

AbstractProstate to bone metastases induce a “vicious cycle” by promoting excessive osteoclast and osteoblast mediated bone degradation and formation that in turn yields factors that drive cancer growth. Recent advances defining the molecular mechanisms that control the vicious cycle have revealed new therapeutic targeting opportunities. However, given the complex temporal and simultaneous cellular interactions occurring in the bone microenvironment, assessing the impact of putative therapies is challenging. To this end, we have integrated biological and computational approaches to generate an accurate model of normal bone matrix homeostasis and the prostate cancer-bone microenvironment. The model faithfully reproduces the basic multicellular unit (BMU) bone coupling process and introduction of a single prostate cancer cell yields a vicious cycle that is similar in cellular composition and pathophysiology to models of prostate to bone metastasis.

scholarly journals Bone metastasis in prostate cancer: Recurring mitochondrial DNA mutation reveals selective pressure exerted by the bone microenvironment

Bone ◽  
2015 ◽  
Vol 78 ◽  
pp. 81-86 ◽  
Author(s):  
Rebecca S. Arnold ◽  
Stacey A. Fedewa ◽  
Michael Goodman ◽  
Adeboye O. Osunkoya ◽  
Haydn T. Kissick ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Mitochondrial Dna ◽  
Bone Metastasis ◽  
Selective Pressure ◽  
Bone Microenvironment ◽  
Mitochondrial Dna Mutation ◽  
Dna Mutation

466: Age is an Independent Predictor of Bone Metastasis at Prostate Cancer Diagnosis

2007 ◽  
Vol 177 (4S) ◽  
pp. 156-156
Author(s):  
Andrea Salonia ◽  
Pierre I. Karakiewicz ◽  
Andrea Gallina ◽  
Alberto Briganti ◽  
Tommaso C. Camerata ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Bone Metastasis ◽  
Cancer Diagnosis ◽  
Independent Predictor ◽  
Prostate Cancer Diagnosis

664: CXCL12/CXCR4 Trans-Activation of HER2 in Lipid Rafts of Prostate Cancer Cell Membranes Promotes Bone Metastasis

2007 ◽  
Vol 177 (4S) ◽  
pp. 223-223
Author(s):  
Sreenivasa R. Chinni ◽  
Hamilto Yamamoto ◽  
Zhong Dong ◽  
Aaron Sabbota ◽  
Sanaa Nabha ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Bone Metastasis ◽  
Lipid Rafts ◽  
Cancer Cell ◽  
Cell Membranes ◽  
Prostate Cancer Cell

790: BMP-7 is a Potent Inhibitor of Prostate Cancer Bone Metastasis in Vivo

2006 ◽  
Vol 175 (4S) ◽  
pp. 255-256
Author(s):  
Cyrill A. Rentsch ◽  
Jeroen Buijs ◽  
Geertje Van der Horst ◽  
Petra Van Overveld ◽  
Antoinette Wetterwald ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Bone Metastasis ◽  
Potent Inhibitor
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