Distribution of somatic H1 subtypes is non-random on active vs. inactive chromatin II: Distribution in human adult fibroblasts

2001 ◽  
Vol 83 (4) ◽  
pp. 643-659 ◽  
Author(s):  
Missag H. Parseghian ◽  
Robert L. Newcomb ◽  
Barbara A. Hamkalo
Keyword(s):  
Human Adult ◽  
Inactive Chromatin ◽  
Adult Fibroblasts

scholarly journals Generation of iPSC line HEL47.2 from healthy human adult fibroblasts

2015 ◽  
Vol 15 (1) ◽  
pp. 263-265 ◽  
Author(s):  
Ras Trokovic ◽  
Jere Weltner ◽  
Timo Otonkoski
Keyword(s):  
Ipsc Line ◽  
Healthy Human ◽  
Human Adult ◽  
Adult Fibroblasts

scholarly journals Reprogramming Human Adult Fibroblasts into GABAergic Interneurons

Cells ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 3450
Author(s):  
Andreas Bruzelius ◽  
Srisaiyini Kidnapillai ◽  
Janelle Drouin-Ouellet ◽  
Tom Stoker ◽  
Roger A. Barker ◽  
...  
Keyword(s):  
Human Fibroblasts ◽  
Autism Spectrum ◽  
Specific Gene ◽  
Gabaergic Interneurons ◽  
Human Adult ◽  
Gene And Protein Expression ◽  
Parkinson’S Diseases ◽  
Fetal Fibroblasts ◽  
Adult Skin ◽  
Adult Fibroblasts

Direct reprogramming is an appealing strategy to generate neurons from a somatic cell by forced expression of transcription factors. The generated neurons can be used for both cell replacement strategies and disease modelling. Using this technique, previous studies have shown that γ-aminobutyric acid (GABA) expressing interneurons can be generated from different cell sources, such as glia cells or fetal fibroblasts. Nevertheless, the generation of neurons from adult human fibroblasts, an easily accessible cell source to obtain patient-derived neurons, has proved to be challenging due to the intrinsic blockade of neuronal commitment. In this paper, we used an optimized protocol for adult skin fibroblast reprogramming based on RE1 Silencing Transcription Factor (REST) inhibition together with a combination of GABAergic fate determinants to convert human adult skin fibroblasts into GABAergic neurons. Our results show a successful conversion in 25 days with upregulation of neuronal gene and protein expression levels. Moreover, we identified specific gene combinations that converted fibroblasts into neurons of a GABAergic interneuronal fate. Despite the well-known difficulty in converting adult fibroblasts into functional neurons in vitro, we could detect functional maturation in the induced neurons. GABAergic interneurons have relevance for cognitive impairments and brain disorders, such as Alzheimer’s and Parkinson’s diseases, epilepsy, schizophrenia and autism spectrum disorders.

scholarly journals MicroRNAs Overcome Cell Fate Barrier by Reducing EZH2-Controlled REST Stability during Neuronal Conversion of Human Adult Fibroblasts

2018 ◽  
Vol 46 (1) ◽  
pp. 73-84.e7 ◽  
Author(s):  
Seong Won Lee ◽  
Young Mi Oh ◽  
Ya-Lin Lu ◽  
Woo Kyung Kim ◽  
Andrew S. Yoo
Keyword(s):  
Cell Fate ◽  
Human Adult ◽  
Neuronal Conversion ◽  
Adult Fibroblasts

scholarly journals MicroRNA-Mediated Direct Reprogramming of Human Adult Fibroblasts Toward Cardiac Phenotype

2020 ◽  
Vol 8 ◽  
Author(s):  
C. Paoletti ◽  
C. Divieto ◽  
G. Tarricone ◽  
F. Di Meglio ◽  
D. Nurzynska ◽  
...  
Keyword(s):  
Direct Reprogramming ◽  
Human Adult ◽  
Cardiac Phenotype ◽  
Adult Fibroblasts
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