Nerve growth factor stimulates the interaction of ZIP/p62 with atypical protein kinase C and targets endosomal localization: Evidence for regulation of nerve growth factor-induced differentiation

2001 ◽  
Vol 82 (3) ◽  
pp. 452-466 ◽  
Author(s):  
Ivy S. Samuels ◽  
M. Lamar Seibenhener ◽  
Kimberly B.W. Neidigh ◽  
Marie W. Wooten
Keyword(s):  
Protein Kinase C ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Protein Kinase ◽  
Atypical Protein Kinase C ◽  
Induced Differentiation ◽  
Nerve Growth ◽  
Atypical Protein Kinase ◽  
Kinase C

scholarly journals The Atypical Protein Kinase C-interacting Protein p62 Is a Scaffold for NF-κB Activation by Nerve Growth Factor

2001 ◽  
Vol 276 (11) ◽  
pp. 7709-7712 ◽  
Author(s):  
Marie W. Wooten ◽  
M. Lamar Seibenhener ◽  
Vidya Mamidipudi ◽  
Maria T. Diaz-Meco ◽  
Philip A. Barker ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Protein Kinase ◽  
Atypical Protein Kinase C ◽  
Interacting Protein ◽  
Nerve Growth ◽  
Atypical Protein Kinase ◽  
Kinase C

scholarly journals Mapping of Atypical Protein Kinase C within the Nerve Growth Factor Signaling Cascade: Relationship to Differentiation and Survival of PC12 Cells

2000 ◽  
Vol 20 (13) ◽  
pp. 4494-4504 ◽  
Author(s):  
Marie W. Wooten ◽  
M. Lamar Seibenhener ◽  
Kimberly B. W. Neidigh ◽  
Michel L. Vandenplas
Keyword(s):  
Protein Kinase C ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Protein Kinase ◽  
Pc12 Cells ◽  
Dominant Negative ◽  
Atypical Protein Kinase C ◽  
Nerve Growth ◽  
Atypical Protein Kinase ◽  
Kinase C

ABSTRACT The pathway by which atypical protein kinase C (aPKC) contributes to nerve growth factor (NGF) signaling is poorly understood. We previously reported that in PC12 cells NGF-induced activation of mitogen-activated protein kinase (MAPK) occurs independently of classical and nonclassical PKC isoforms, whereas aPKC isoforms were shown to be required for NGF-induced differentiation. NGF-induced activation of PKC-ι was observed to be dependent on phosphatidylinositol 3-kinase (PI3K) and led to coassociation of PKC-ι with Ras and Src. Expression of dominant negative mutants of either Src (DN2) or Ras (Asn-17) impaired activation of PKC-ι by NGF. At the level of Raf-1, neither PKC-ι nor PI3 kinase was required for activation; however, PKC-ι could weakly activate MEK. Inhibitors of PKC-ι activity and PI3K had no effect on NGF-induced MAPK or p38 activation but reduced NGF-stimulated c-Jun N-terminal kinase activity. Src, PI3K, and PKC-ι were likewise required for NGF-induced NF-κB activation and cell survival, whereas Ras was not required for either survival or NF-κB activation but was required for differentiation. IKK existed as a complex with PKC-ι, Src and IκB. Consistent with a role for Src in regulating NF-κB activation, an absence of Src activity impaired recruitment of PKC-ι into an IKK complex and markedly impaired NGF-induced translocation of p65/NF-κB to the nucleus. These findings reveal that in PC12 cells, aPKCs comprise a molecular switch to regulate differentiation and survival responses coupled downstream to NF-κB. On the basis of these findings, Src emerges as a critical upstream regulator of both PKC-ι and the NF-κB pathway.

Nerve growth factor activates kinases that phosphorylate atypical protein kinase C

2002 ◽  
Vol 14 (4) ◽  
pp. 359-363 ◽  
Author(s):  
Michel L Vandenplas ◽  
Vidya Mamidipudi ◽  
M Lamar Seibenhener ◽  
Marie W Wooten
Keyword(s):  
Protein Kinase C ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Protein Kinase ◽  
Atypical Protein Kinase C ◽  
Nerve Growth ◽  
Atypical Protein Kinase ◽  
Kinase C

Nerve growth factor enhances the level of the protein kinase C substrate B-50 in pheochromocytoma PC12 cells

1986 ◽  
Vol 139 (2) ◽  
pp. 644-651 ◽  
Author(s):  
C.O.M. Van Hooff ◽  
P.N.E. De Graan ◽  
J. Boonstra ◽  
A.B. Oestreicher ◽  
M.H. Schmidt-Michels ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Protein Kinase ◽  
Pc12 Cells ◽  
Nerve Growth ◽  
Kinase C ◽  
Pheochromocytoma Pc12 ◽  
Pheochromocytoma Pc12 Cells
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