An insight into the mechanism of cytotoxicity of ricin to hepatoma cell: Roles of Bcl-2 family proteins, caspases, Ca2+-dependent proteases and protein kinase C

2001 ◽  
Vol 81 (4) ◽  
pp. 583-593 ◽  
Author(s):  
Rong-gui Hu ◽  
Qi-wei Zhai ◽  
Wang-yi Liu ◽  
Xin-yuan Liu
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Hepatoma Cell ◽  
Kinase C ◽  
Insight Into

scholarly journals Keratin 8/18 Modulation of Protein Kinase C-mediated Integrin-dependent Adhesion and Migration of Liver Epithelial Cells

2010 ◽  
Vol 21 (10) ◽  
pp. 1698-1713 ◽  
Author(s):  
François Bordeleau ◽  
Luc Galarneau ◽  
Stéphane Gilbert ◽  
Anne Loranger ◽  
Normand Marceau
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Epithelial Cells ◽  
Time Course ◽  
Cell Attachment ◽  
Hepatoma Cell ◽  
Focal Adhesions ◽  
Regulatory Function ◽  
Kinase C ◽  
And Migration

Keratins are intermediate filament (IF) proteins of epithelial cells, expressed as pairs in a lineage/differentiation manner. Hepatocyte and hepatoma cell IFs are made solely of keratins 8/18 (K8/K18), the hallmark of all simple epithelia. Cell attachment/spreading (adhesion) and migration involve the formation of focal adhesions at sites of integrin interactions with extracellular matrix, actin adaptors such as talin and vinculin, and signaling molecules such as focal adhesion kinase (FAK) and member(s) of the protein kinase C (PKC) family. Here, we identify the novel PKCδ as mediator of the K8/K18 modulation of hepatoma cell adhesion and migration. We also demonstrate a K8/K18-dependent relationship between PKCδ and FAK activation through an integrin/FAK-positive feedback loop, in correlation with a reduced FAK time residency at focal adhesions. Notably, a K8/K18 loss results to a time course modulation of the receptor of activated C-kinase-1, β1-integrin, plectin, PKC, and c-Src complex formation. Although the K8/K18 modulation of hepatocyte adhesion also occurs through a PKC mediation, these differentiated epithelial cells exhibit minimal migrating ability, in link with marked differences in protein partner content and distribution. Together, these results uncover a key regulatory function for K8/K18 IFs in the PKC-mediated integrin/FAK-dependent adhesion and migration of simple epithelial cells.

Sign in / Sign up

Export Citation Format

Share Document