Understanding immune cell trafficking patterns via in vivo bioluminescence imaging

2002 ◽  
Vol 87 (S39) ◽  
pp. 239-248 ◽  
Author(s):  
Stefanie Mandl ◽  
Christoph Schimmelpfennig ◽  
Matthias Edinger ◽  
Robert S. Negrin ◽  
Christopher H. Contag
Keyword(s):  
Bioluminescence Imaging ◽  
Immune Cell ◽  
Cell Trafficking ◽  
Immune Cell Trafficking ◽  
In Vivo Bioluminescence Imaging

Revealing lymphoma growth and the efficacy of immune cell therapies using in vivo bioluminescence imaging

Blood ◽  
2003 ◽  
Vol 101 (2) ◽  
pp. 640-648 ◽  
Author(s):  
Matthias Edinger ◽  
Yu-An Cao ◽  
Michael R. Verneris ◽  
Michael H. Bachmann ◽  
Christopher H. Contag ◽  
...  
Keyword(s):  
T Cells ◽  
Tumor Growth ◽  
Bioluminescence Imaging ◽  
Immune Cell ◽  
Residual Disease ◽  
Cell Therapies ◽  
Nk T Cells ◽  
In Vivo Bioluminescence Imaging ◽  
Minimal Residual

Cancer therapeutics have achieved success in the treatment of a variety of malignancies, however, relapse of disease from small numbers of persistent tumor cells remains a major obstacle. Advancement of treatment regimens that effectively control minimal residual disease and prevent relapse would be greatly accelerated if sensitive and noninvasive assays were used to quantitatively assess tumor burden in animal models of minimal residual disease that are predictive of the human response. In vivo bioluminescence imaging (BLI) is an assay for the detection of small numbers of cells noninvasively and enables the quantification of tumor growth within internal organs. Fusion genes that encode bioluminescent and fluorescent reporter proteins effectively couple the powerful in vivo capabilities of BLI with the subset-discriminating capabilities of fluorescence-activated cell sorting. We labeled 2 murine lymphoma cell lines with dual function reporter genes and monitored radiation and chemotherapy as well as immune-based strategies that employ the tumorcidal activity of ex vivo–expanded CD8+ natural killer (NK)–T cells. Using BLI we were able to visualize the entire course of malignant disease including engraftment, expansion, metastasis, response to therapy, and unique patterns of relapse. We also labeled the effector NK-T cells and monitored their homing to the sites of tumor growth followed by tumor eradication. These studies reveal the efficacy of immune cell therapies and the tempo of NK-T cell trafficking in vivo. The complex cellular processes in bone marrow transplantation and antitumor immunotherapy, previously inaccessible to investigation, can now be revealed in real time in living animals.

In vivo imaging of immune cell trafficking in cancer

2010 ◽  
Vol 38 (5) ◽  
pp. 949-968 ◽  
Author(s):  
Luisa Ottobrini ◽  
Cristina Martelli ◽  
Daria Lucia Trabattoni ◽  
Mario Clerici ◽  
Giovanni Lucignani
Keyword(s):  
In Vivo Imaging ◽  
Immune Cell ◽  
Cell Trafficking ◽  
Immune Cell Trafficking

scholarly journals A Dynamic in vitro BBB Model for the Study of Immune Cell Trafficking into the Central Nervous System

2010 ◽  
Vol 31 (2) ◽  
pp. 767-777 ◽  
Author(s):  
Luca Cucullo ◽  
Nicola Marchi ◽  
Mohammed Hossain ◽  
Damir Janigro
Keyword(s):  
Immune Cells ◽  
Immune Cell ◽  
Cell Trafficking ◽  
In Vitro System ◽  
Immune Cell Trafficking ◽  
The Central Nervous System ◽  
In Vitro Bbb Model ◽  
The Brain

Although there is significant evidence correlating overreacting or perhaps misguided immune cells and the blood–brain barrier (BBB) with the pathogenesis of neuroinflammatory diseases, the mechanisms by which they enter the brain are largely unknown. For this purpose, we revised our humanized dynamic in vitro BBB model (DIV-BBBr) to incorporate modified hollow fibers that now feature transmural microholes (2 to 4 μm Ø) allowing for the transendothelial trafficking of immune cells. As with the original model, this new DIV-BBBr reproduces most of the physiological characteristics of the BBB in vivo. Measurements of transendothelial electrical resistance (TEER), sucrose permeability, and BBB integrity during reversible osmotic disruption with mannitol (1.6 mol/L) showed that the microholes do not hamper the formation of a tight functional barrier. The in vivo rank permeability order of sucrose, phenytoin, and diazepam was successfully reproduced in vitro. Flow cessation followed by reperfusion (Fc/Rp) in the presence of circulating monocytes caused a biphasic BBB opening paralleled by a significant increase of proinflammatory cytokines and activated matrix metalloproteinases. We also observed abluminal extravasation of monocytes but only when the BBB was breached. In conclusion, the DIV-BBBr represents the most realistic in vitro system to study the immune cell trafficking across the BBB.

The Antitumor Effects of Britanin on Hepatocellular Carcinoma Cells and its Real-Time Evaluation by In Vivo Bioluminescence Imaging

2020 ◽  
Vol 20 (9) ◽  
pp. 1147-1156
Author(s):  
Hanrui Li ◽  
GeTao Du ◽  
Lu Yang ◽  
Liaojun Pang ◽  
Yonghua Zhan
Keyword(s):  
Hepatocellular Carcinoma ◽  
Western Blot ◽  
Blot Analysis ◽  
Tumor Size ◽  
Hepg2 Cells ◽  
Western Blot Analysis ◽  
Bioluminescence Imaging ◽  
Antitumor Effects ◽  
In Vivo Bioluminescence Imaging

Background: Hepatocellular carcinoma is cancer with many new cases and the highest mortality rate. Chemotherapy is the most commonly used method for the clinical treatment of hepatocellular carcinoma. Natural products have become clinically important chemotherapeutic drugs due to their great potential for pharmacological development. Many sesquiterpene lactone compounds have been proven to have antitumor effects on hepatocellular carcinoma. Objective: Britanin is a sesquiterpene lactone compound that can be considered for the treatment of hepatocellular carcinoma. The present study aimed to investigate the antitumor effect of britanin. Methods: BEL 7402 and HepG2 cells were used to study the cytotoxicity and antitumor effects of britanin. Preliminary studies on the nuclear factor kappa B pathway were conducted by western blot analysis. A BEL 7402-luc subcutaneous tumor model was established for the in vivo antitumor studies of britanin. In vivo bioluminescence imaging was conducted to monitor changes in tumor size. Results: The results of the cytotoxicity analysis showed that the IC50 values for britanin in BEL 7402 and HepG2 cells were 2.702μM and 6.006μM, respectively. The results of the colony formation demonstrated that the number of cells in a colony was reduced significantly after britanin treatment. And the results of transwell migration assays showed that the migration ability of tumor cells was significantly weakened after treatment with britanin. Tumor size measurements and staining results showed that tumor size was inhibited after britanin treatment. The western blot analysis results showed the inhibition of p65 protein expression and reduced the ratio of Bcl-2/Bax after treatment. Conclusion: A series of in vitro and in vivo experiments demonstrated that britanin had good antitumor effects and provided an option for hepatocellular carcinoma treatment.

In Vivo Bioluminescence Imaging of Transplanted Islets and Early Detection of Graft Rejection

2006 ◽  
Vol 81 (10) ◽  
pp. 1421-1427 ◽  
Author(s):  
Xiaojuan Chen ◽  
Xiaomin Zhang ◽  
Courtney S. Larson ◽  
Marshall S. Baker ◽  
Dixon B. Kaufman
Keyword(s):  
Early Detection ◽  
Graft Rejection ◽  
Bioluminescence Imaging ◽  
In Vivo Bioluminescence Imaging

scholarly journals An Advanced Preclinical Mouse Model for Acute Myeloid Leukemia Using Patients' Cells of Various Genetic Subgroups and In Vivo Bioluminescence Imaging

PLoS ONE ◽  
2015 ◽  
Vol 10 (3) ◽  
pp. e0120925 ◽  
Author(s):  
Binje Vick ◽  
Maja Rothenberg ◽  
Nadine Sandhöfer ◽  
Michela Carlet ◽  
Cornelia Finkenzeller ◽  
...  
Keyword(s):  
Acute Myeloid Leukemia ◽  
Mouse Model ◽  
Myeloid Leukemia ◽  
Bioluminescence Imaging ◽  
In Vivo Bioluminescence Imaging ◽  
Acute Myeloid

HEVs, lymphatics and homeostatic immune cell trafficking in lymph nodes

2012 ◽  
Vol 12 (11) ◽  
pp. 762-773 ◽  
Author(s):  
Jean-Philippe Girard ◽  
Christine Moussion ◽  
Reinhold Förster
Keyword(s):  
Lymph Nodes ◽  
Immune Cell ◽  
Cell Trafficking ◽  
Immune Cell Trafficking
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