Differential regulation of Cbfa1/Runx2 and osteocalcin gene expression by vitamin-D3, dexamethasone, and local growth factors in primary human osteoblasts

2002 ◽  
Vol 86 (2) ◽  
pp. 348-356 ◽  
Author(s):  
Volker Viereck ◽  
Heide Siggelkow ◽  
Simone Tauber ◽  
Dirk Raddatz ◽  
Norbert Schutze ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Factors ◽  
Vitamin D3 ◽  
Differential Regulation ◽  
Local Growth ◽  
Human Osteoblasts ◽  
Osteocalcin Gene ◽  
Primary Human Osteoblasts

scholarly journals Adhesion Behaviour of Primary Human Osteoblasts and Fibroblasts on Polyether Ether Ketone Compared with Titanium under In Vitro Lipopolysaccharide Incubation

Materials ◽  
2019 ◽  
Vol 12 (17) ◽  
pp. 2739 ◽  
Author(s):  
Korbinian Benz ◽  
Andreas Schöbel ◽  
Marisa Dietz ◽  
Peter Maurer ◽  
Jochen Jackowski
Keyword(s):  
Gene Expression ◽  
Protein Level ◽  
Binding Protein ◽  
Sem Images ◽  
Human Osteoblasts ◽  
Polyether Ether Ketone ◽  
Primary Human Osteoblasts ◽  
Titanium Surfaces ◽  
Ether Ketone

The aim of this in vitro pilot study was to analyse the adhesion behaviour of human osteoblasts and fibroblasts on polyether ether ketone (PEEK) when compared with titanium surfaces in an inflammatory environment under lipopolysaccharide (LPS) incubation. Scanning electron microscopy (SEM) images of primary human osteoblasts/fibroblasts on titanium/PEEK samples were created. The gene expression of the LPS-binding protein (LBP) and the LPS receptor (toll-like receptor 4; TLR4) was measured by real-time polymerase chain reaction (PCR). Immunocytochemistry was used to obtain evidence for the distribution of LBP/TLR4 at the protein level of the extra-cellular-matrix-binding protein vinculin and the actin cytoskeleton. SEM images revealed that the osteoblasts and fibroblasts on the PEEK surfaces had adhesion characteristics comparable to those of titanium. The osteoblasts contracted under LPS incubation and a significantly increased LBP gene expression were detected. This was discernible at the protein level on all the materials. Whereas no increase of TLR4 was detected with regard to mRNA concentrations, a considerable increase in the antibody reaction was detected on all the materials. As is the case with titanium, the colonisation of human osteoblasts and fibroblasts on PEEK samples is possible under pro-inflammatory environmental conditions and the cellular inflammation behaviour towards PEEK is lower than that of titanium.

scholarly journals Microarray analysis of gene expression in primary human osteoblasts derived from spine

2012 ◽  
Vol 20 ◽  
pp. S197-S198
Author(s):  
F. Moldovan ◽  
K. Fendri ◽  
S. Parent ◽  
C. Zaouter ◽  
A. Moreau ◽  
...  
Keyword(s):  
Gene Expression ◽  
Microarray Analysis ◽  
Human Osteoblasts ◽  
Primary Human Osteoblasts

Effects of glucocorticoids on vitamin D3-metabolizing 24-hydroxylase (CYP24A1) in Saos-2 cells and primary human osteoblasts

2019 ◽  
Vol 496 ◽  
pp. 110525 ◽  
Author(s):  
Ahmad Zayny ◽  
Mokhtar Almokhtar ◽  
Kjell Wikvall ◽  
Östen Ljunggren ◽  
Kumari Ubhayasekera ◽  
...  
Keyword(s):  
Vitamin D3 ◽  
Human Osteoblasts ◽  
Primary Human Osteoblasts

Simvastatin and atorvastatin enhance gene expression of collagen type 1 and osteocalcin in primary human osteoblasts and MG-63 cultures

2007 ◽  
Vol 101 (6) ◽  
pp. 1430-1438 ◽  
Author(s):  
Silvia Ruiz-Gaspa ◽  
Xavier Nogues ◽  
Anna Enjuanes ◽  
Joan C. Monllau ◽  
Josep Blanch ◽  
...  
Keyword(s):  
Gene Expression ◽  
Collagen Type ◽  
Human Osteoblasts ◽  
Enhance Gene Expression ◽  
Primary Human Osteoblasts ◽  
Collagen Type 1
Sign in / Sign up

Export Citation Format

Share Document