Automated red blood cell exchange in preparation for filgrastim mobilization of autologous peripheral blood hematopoietic progenitor cells in a patient with sickle cell anemia

2017 ◽  
Vol 33 (3) ◽  
pp. 431-435 ◽  
Author(s):  
Yong Zhao ◽  
Jeffrey A. Bailey ◽  
Jeanne Linden ◽  
Patricia St. Pierre ◽  
Jan Cerny ◽  
...  
Keyword(s):  
Blood Cell ◽  
Progenitor Cells ◽  
Sickle Cell ◽  
Sickle Cell Anemia ◽  
Red Blood Cell ◽  
Peripheral Blood ◽  
Hematopoietic Progenitor Cells ◽  
Hematopoietic Progenitor

scholarly journals Arhgap21+/- Mice Show Impaired Adhesion, Migration, Homing and Short Term Reconstitution of Hematopoietic Progenitor Cells

Blood ◽  
2014 ◽  
Vol 124 (21) ◽  
pp. 4333-4333
Author(s):  
Juliana M. Xavier ◽  
Ricon Lauremília ◽  
Vieira Karla ◽  
Longhini Ana Leda ◽  
Franchi Gilberto ◽  
...  
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Progenitor Cells ◽  
Red Blood Cell ◽  
Peripheral Blood ◽  
Hematopoietic Progenitor Cells ◽  
Blood Cell Count ◽  
Hematopoietic Progenitor ◽  
Short Term

Abstract The main signaling pathway involved in migration, adhesion and homing of hematopoietic progenitor cells (HPC) is the CXCL-12, chemokine produced by bone marrow (BM) stromal cells that bind to its main receptor CXCR-4, expressed by HPC. ARHGAP21 is a RhoGAP member, negative regulators of RhoGTPAses. It was described that ARHGAP21 is involved in cellular migration and adhesion but its role in hematopoiesis is unknown. Researching Arhgap21 role in hematopoiesis, Arhgap21+/- mice were generated using Embryonic Stem cell obtained from GeneTrap consortium. Hematologic parameters were investigated by blood cell count, colony formation assay in methylcellulose medium and immunophenotipic characterization of hematopoietic populations. Transwell migration and adhesion of Lin- cells assay in the presence of CXCL12 were also performed. In vivo analysis of Arhgap21+/- HPC was achieved with homing and CFU-S assays in sublethally irradiated mice and hematopoiesis stress was generated by 5-fluoracil treatment. Arhgap21+/- mice presented more than 50% reduction in Arhgap21 expression (0.47 ± 0.13) in BM when compared to WT (1 ± 0.03) and live in normal conditions. On the other hand, Arhgap21 mice showed a reduction in BFU-E colonies and erythroid (Terr119+) committed cells in BM together with decreased peripheral blood (PB) Terr119+ cells and red blood cell number and increased medium corpuscular volume. In myeloid compartment, Arhgap21+/- mice presented less GM colonies and myeloid (Gr1/Mac1+) cells in BM followed by an increase of these cells in PB suggesting myeloid mobilization. Corroborating to this, whenArhgap21+/- BM was challenged for hematopoiesis stress with 5FU, the animals showed increased neutrophil number in peripheral blood 14 days (WT: 1887/mm3 ±721.9; Arhgap21+/-:3325/mm3 ±1640, p=0.02) and 21days (WT: 1264/mm3 ±635; Arhgap21+/-: 2182/mm3 ±854, p=0.01) after treatment, together with increased number of LSK cells in the BM (WT: 1.3% ±0.4; Arhgap21+/-: 1.9% ±0.3, p=0.006) 28 days after 5FU infusion. Erythroid and myeloid compartments reductions were observed together with an increase of Arhgap21+/- BM short term LSK( Arhgap21+/- : 0.34% ±0.13; WT: 0.18% ±0.07, p=0.03) and long term LSK (Arhgap21+/-: 0.003% ±0.001; WT: 0.002 ±0.0007, p=0.02) suggesting an attempt to restore normal hematopoietic levels. Arhgap21+/- HPC showed reduced CXCL-12-induced migration compared to WT (WT: 100%, Arhgap21+/-: 54.73% ± 13.57, p=0.01) in addition to decreased adhesion to fibronectin (Arhgap21+/-: 15% ± 3.8; WT: 27% ± 3.5, p=0.003) and a4b1 integrin expression (WT: 83.76 ± 4.35; Arhgap21+/-: 71.06 ± 7.00, p=0.008). In homing assay, the percentage of donor Lin- HPC from Arhgap21+/- mice that homed to BM (6.32 ± 2.41) or spleen (3.48 ± 1.57), were lower than those from WT mice (BM=10.09 ± 1.81;p=0.004; spleen=6.9 ± 1.48; p=0.0007) together with higher frequency of these cells in peripheral blood (WT: 6.39 ± 3.38; Arhgap21+/-: 13.95 ± 5.33, p=0.003), suggesting a retention of Arhgap21+/- HPC in the bloodstream, which inefficiently home to BM and spleen. Arhgap21+/- CFU-S capacity decline was also observed (Arhgap21+/-: 20.86 ± 2; WT: 29.29 ± 5.4 p=0.002). This is the first study showing that ARHGAP21 is involved in hematopoiesis and also shows that ARHGAP21 is an important protein for chemotaxis, adhesion, homing and short term reconstitution of HPC. Table. Hematological Parameters WT Arhgap21+/- Hemoglobin (g/dL) * 16.19 ± 0.35 15.62±0.32 RBC (10^6/uL) * 10.91 ± 0.3 10.46±0.25 Hematocrit (%) 54.78 ± 1.6 53.75± 1.3 MCV (fL) * 50 ±0.4 50.08±0.74 WBC (10^3/Ul) * 10.08 ±2 12.9±1.8 Platelets(10^3/uL) 1793 ±114.3 1770±96.5 Myeloid, Gr1+Mac1+ in BM (%) * 47.27 ±4.7 40.88 ±6.23 Erythroid, Terr119+ in BM (%) * 9.98 ±4.1 6.4 ±1.5 Short Term HSC, LSK in BM (%) * 0.18 ±0.07 0.34 ±0.13 Long Term HSC, LSK CD150+CD48- in BM (%) * 0.002 ±0.0007 0.003 ±0.001 Myeloid, Gr1+Mac1+ in PB (%) * 7.7 ±1.5 13.1 ±2.6 Erythroid, Terr119+ in PB (%) * 38.7 ±4.4 28.4 ±8.7 Myeloid Colonies, CFU-GM (number) 66.7 ±14.7 48.07 ±12.5 Erythroid Colonies, BFU-E (number) 5.5 ±2.2 3.2 ±1.5 Blood samples were collected from 9 WT and 8 Arhgap21+/- mice. RBC: red blood cell count; MCV: mean cell volume; WBC: White blood cell count. Hematopoietic populations were analyzed in flow cytometry and colony formation in methylcellulose assay. Data are expressed as mean ± standard deviation, analyzed by Student t test and considered statistically significant (*) if p ≤ 0.05. Disclosures No relevant conflicts of interest to declare.

Diagnosis support of sickle cell anemia by classifying red blood cell shape in peripheral blood images

2020 ◽  
Vol 58 (6) ◽  
pp. 1265-1284
Author(s):  
Wilkie Delgado-Font ◽  
Miriela Escobedo-Nicot ◽  
Manuel González-Hidalgo ◽  
Silena Herold-Garcia ◽  
Antoni Jaume-i-Capó ◽  
...  
Keyword(s):  
Blood Cell ◽  
Sickle Cell ◽  
Sickle Cell Anemia ◽  
Red Blood Cell ◽  
Peripheral Blood ◽  
Cell Shape

scholarly journals Transplantation of enriched and purged peripheral blood progenitor cells from a single apheresis product in patients with non-Hodgkin's lymphoma

Blood ◽  
1995 ◽  
Vol 85 (11) ◽  
pp. 3334-3341 ◽  
Author(s):  
RS Negrin ◽  
CR Kusnierz-Glaz ◽  
BJ Still ◽  
JR Schriber ◽  
NJ Chao ◽  
...  
Keyword(s):  
Blood Cell ◽  
Progenitor Cells ◽  
Tumor Cells ◽  
Peripheral Blood ◽  
Hodgkin’S Lymphoma ◽  
Hodgkin's Lymphoma ◽  
Hematopoietic Progenitor Cells ◽  
Hematopoietic Progenitor ◽  
Non Hodgkin's Lymphoma ◽  
Cd34 Cells

High-dose chemotherapy with or without radiotherapy followed by autologous transplantation of hematopoietic progenitor cells is an effective treatment for patients with high-risk or relapsed non- Hodgkin's lymphoma. Chemotherapy and/or hematopoietic growth factors have been used to mobilize progenitor cells in the peripheral blood for transplantation. However, the mobilized blood cell products have been found to be frequently contaminated with tumor cells, and techniques have not been developed to purge tumor cells from these products. In addition, the minimum number of hematopoietic progenitor cells required for engraftment has not yet been fully elucidated. We treated 21 patients with a single infusion of cyclophosphamide (4 g/m2) followed by daily administration of granulocyte colony-stimulating factor (G-CSF). After recovery of the white blood cell count, a single 3-hour apheresis collection was performed. The apheresis product was then applied to a discontinuous Percoll gradient. The low-density fractions resulting from this separation procedure were enriched for CD34+ progenitor cells (total cell yield, 19.5%; CD34+ cell recovery, 81.2%). These enriched cellular products were treated with a panel of anti-B cell or anti-T cell monoclonal antibodies and complement in an effort to remove residual tumor cells. After treatment of the patient with myeloablative therapies, the enriched and purged cells were reinfused. Hematologic recovery was rapid, with median neutrophil engraftment in 10 days [absolute neutrophil count (ANC), greater than 0.5 x 10(9)/L] and 11 days (ANC, greater than 1.0 x 10(9)/L). Median platelet transfusion independence required 13 days. The rapidity of multilineage engraftment correlated with the number of CD34+ cells per kilogram that were infused. Patients who received more than 2 x 10(6) CD34+ cells per kilogram had rapid hematologic engraftment, whereas those patients transplanted with less than 2 x 10(6) CD34+ cells per kilogram had slower platelet recovery. Modeling studies using a lymphoma cell line with a t(14; 18) chromosomal translocation demonstrated the successful removal of tumor cells assayed using the polymerase chain reaction (PCR) after the processing and purging. Four of the 21 patients had PCR-detectable lymphoma cells in the bone marrow and peripheral blood; however, the enriched and purged blood products reinfused in all four did not contain detectable tumor cells.(ABSTRACT TRUNCATED AT 400 WORDS)

Prevalence of Red Blood Cell Alloimmunization Among Beta-Thalassemia and Sickle Cell Anemia Patients: a Study from Saudi Arabia

2021 ◽  
Vol 67 (10/2021) ◽  
Author(s):  
Raed Felimban ◽  
Ahmed Alsharyufi ◽  
Jasem Aljehani ◽  
Ahmed Sahlool ◽  
Hamead Aljabri ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Blood Cell ◽  
Sickle Cell ◽  
Sickle Cell Anemia ◽  
Red Blood Cell ◽  
Beta Thalassemia

Alpha thalassemia protects sickle cell anemia patients from macro-albuminuria through its effects on red blood cell rheological properties

2014 ◽  
Vol 57 (1) ◽  
pp. 63-72 ◽  
Author(s):  
Yann Lamarre ◽  
Marc Romana ◽  
Nathalie Lemonne ◽  
Marie-Dominique Hardy-Dessources ◽  
Vanessa Tarer ◽  
...  
Keyword(s):  
Blood Cell ◽  
Rheological Properties ◽  
Sickle Cell ◽  
Sickle Cell Anemia ◽  
Red Blood Cell ◽  
Alpha Thalassemia

scholarly journals The Effects of Cyanate In Vitro on Red Blood Cell Metabolism and Function in Sickle Cell Anemia

1972 ◽  
Vol 51 (3) ◽  
pp. 566-574 ◽  
Author(s):  
Frank G. De Furia ◽  
Denis R. Miller ◽  
Anthony Cerami ◽  
James M. Manning
Keyword(s):  
Blood Cell ◽  
Sickle Cell ◽  
Sickle Cell Anemia ◽  
Red Blood Cell ◽  
Cell Metabolism ◽  
And Function
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