Large-volume leukapheresis using femoral venous access for harvesting peripheral blood stem cells with the Fenwal CS 3000 Plus from normal healthy donors: Predictors of CD34+ cell yield and collection efficiency

2003 ◽  
Vol 18 (1) ◽  
pp. 10-15 ◽  
Author(s):  
Sang Kyun Sohn ◽  
Jong Gwang Kim ◽  
Yeo Soo Chae ◽  
Dong Hwan Kim ◽  
Nan Young Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Large Volume ◽  
Peripheral Blood ◽  
Collection Efficiency ◽  
Venous Access ◽  
Cell Yield ◽  
Peripheral Blood Stem Cells ◽  
Healthy Donors ◽  
Blood Stem Cells

Mobilization of Allogeneic Peripheral Blood Stem Cells in Family Donors with Single-Dose Pegfilgrastim.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 2148-2148
Author(s):  
Vladan Vucinic ◽  
Nadezda Basara ◽  
Runa Stiegler ◽  
Kristina Bartsch ◽  
Constanze Kliem ◽  
...  
Keyword(s):  
Stem Cells ◽  
Body Weight ◽  
Single Dose ◽  
Peripheral Blood ◽  
Peripheral Blood Stem Cells ◽  
Weight Range ◽  
Healthy Donors ◽  
Cd34 Cells ◽  
Blood Stem Cells ◽  
Pbsc Mobilization

Abstract Abstract 2148 Poster Board II-125 Introduction: The standard procedure for obtaining peripheral blood stem cells (PBSC) is donor mobilization with G-CSF. Pegfilgrastim is a covalently bound conjugate of filgrastim and monomethoxypolyethylene glycol with longer half-life elimination due to decreased plasma clearance and could represent an alternative approach for PBSC mobilization in healthy donors. Design and Methods: From July 2006 till August 2009 28 related healthy donors (50% male, 50% female) were treated with single dose of 12 mg pegfilgrastim for mobilization of allogeneic PBSC. The harvests were performed as large-volume, continuous-flow collections using a Cobe Spectra blood cell separator on day 4 and if necessary on day 5 of the mobilization regimen. In case of inadequate CD34+ counts (less than 4×106/kg body weight of recipient on day 5), stimulation was continued with filgrastim. In addition, the serum level of filgrastim was determined twice daily. Results: We present the results of 27 donors (the results of the 28th donor are still pending). In all 27 cases the harvests were successful. In 22 out of 27 donors (82%) only a single apheresis was needed to reach the target. Two of the donors required additional treatment with non-pegylated filgrastim. The maximal concentration of circulating CD34+ cells was achieved on day 4 (median 74.3/μl; range 24.6-136.6). The median yield of CD34+ cells was 5.9×106/kg of the recipients body weight (range 3-14.5), and the median CD3+ count was 9.1×108/kg of the recipient body weight (range 1.4-6.2). Serum filgrastim level peak was on day 2 of the mobilization regimen with a median level of 226 ng/ml (range 35 to 1123 ng/ml), thus preceding the increase of CD34+ cells in blood. The main adverse events were WHO grade 1 and included headaches, bone pain and transient elevations of alkaline phosphatase and lactate dehydrogenase. Conclusion: PBSC mobilization with a single dose of pegfilgrastim is feasible for healthy donors. The graft composition was comparable to that obtained with the conventional regimen of short-term G-CSF. Long-term follow-up of healthy donors treated with pegfilgrastim should be further investigated. Disclosures: No relevant conflicts of interest to declare.

Engraftment with peripheral blood stem cells collected by large-volume leukapheresis for patients with lymphoma

Transfusion ◽  
2003 ◽  
Vol 32 (8) ◽  
pp. 729-731 ◽  
Author(s):  
R.L. COMENZO ◽  
M.E. MALACHOWSKI ◽  
K.B. MILLER ◽  
J.J. ERBAN ◽  
D.P. SCHENKEIN ◽  
...  
Keyword(s):  
Stem Cells ◽  
Large Volume ◽  
Peripheral Blood ◽  
Peripheral Blood Stem Cells ◽  
Blood Stem Cells

Mobilization Factors of Peripheral Blood Stem Cells in Healthy Donors

2002 ◽  
Vol 6 (6) ◽  
pp. 413-418 ◽  
Author(s):  
N. Shimizu ◽  
T. Asai ◽  
S. Hashimoto ◽  
M. Narita ◽  
M. Kobayashi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Peripheral Blood ◽  
Peripheral Blood Stem Cells ◽  
Healthy Donors ◽  
Blood Stem Cells

Effective collection of peripheral blood stem cells in children weighing 20 kilogram or less in a single large-volume apheresis procedure

2014 ◽  
Vol 30 (5) ◽  
pp. 281-287 ◽  
Author(s):  
Rosario Salazar-Riojas ◽  
José Alberto García-Lozano ◽  
Mayra Valdés-Galván ◽  
Odra Martínez-González ◽  
Olga Graciela Cantú-Rodríguez ◽  
...  
Keyword(s):  
Stem Cells ◽  
Large Volume ◽  
Peripheral Blood ◽  
Peripheral Blood Stem Cells ◽  
Blood Stem Cells

scholarly journals A Method to Predict the Yield of Peripheral Blood Stem Cells Collected by Large Volume Leukapheresis

2017 ◽  
Vol 07 (01) ◽  
Author(s):  
Chen J ◽  
Burns K ◽  
Kennedy M ◽  
Segura FJ ◽  
Garcia S ◽  
...  
Keyword(s):  
Stem Cells ◽  
Large Volume ◽  
Peripheral Blood ◽  
Peripheral Blood Stem Cells ◽  
Blood Stem Cells
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