Excess hydrogen peroxide inhibits head and foot regeneration in hydra by affecting DNA repair and expression of essential genes

2020 ◽  
Vol 34 (11) ◽  
Author(s):  
Gauri A. Haval ◽  
Komal D. Pekhale ◽  
Nusrat A. Perween ◽  
Surendra M. Ghaskadbi ◽  
Saroj S. Ghaskadbi
Keyword(s):  
Hydrogen Peroxide ◽  
Dna Repair ◽  
Essential Genes ◽  
Excess Hydrogen

scholarly journals Fluorescent Derivatization of Aromatic Carboxylic Acids with Horseradish Peroxidase in the Presence of Excess Hydrogen Peroxide

2015 ◽  
Vol 31 (1) ◽  
pp. 37-44
Author(s):  
Junichi ODO ◽  
Masahiko INOGUCHI ◽  
Hiroyuki AOKI ◽  
Yuto SOGAWA ◽  
Masahiro NISHIMURA
Keyword(s):  
Hydrogen Peroxide ◽  
Horseradish Peroxidase ◽  
Carboxylic Acids ◽  
Aromatic Carboxylic Acids ◽  
Excess Hydrogen

Influence of Natural Organic Matter on Oxidation of Volatile Organic Compounds by the H2O2/VisUV Process

1997 ◽  
Vol 2 (3) ◽  
Author(s):  
James M. Symons ◽  
Charlene M. Baker ◽  
H. William Prengle
Keyword(s):  
Hydrogen Peroxide ◽  
Rate Constant ◽  
Reaction Rate ◽  
Tap Water ◽  
Reaction Rate Constant ◽  
Photon Flux ◽  
Excess Hydrogen ◽  
Volatile Organic ◽  
Natural Organic Material ◽  
Experimental Runs

AbstractThis paper presents experimental research to determine the affect of background natural organic material (NOM) on the conversion of five (5) VOC's: 1,1,1-trichloroethane (TCA), benzene (BNZ), trichloroethylene (TCE), 1,4-dichlorobenzene (DCB), and tetrachloroethylene (PCE). Experiments were conducted using DI water and Houston tap water ([TOC] = 3.6 mg/L) as solvents. In addition, the affects of buffer form and excess hydrogen peroxide were determined. Experimental runs were conducted in a photochemical-flow-stirred-tank reactor (pcfSTR), using a 450 W visible/ultraviolet radiation source. The data were analyzed using the Prengle- Shimoda reaction rate model, yielding the reaction rate constant ka (μmols A conv/min, Lr, photon flux) for comparison purposes. Analysis of the experimental data indicated the following conclusions: 1) At the concentrations used for bicarbonate or phosphate buffer, little or no affect was observed; 2) The presence of NOM surpressed the reaction rate for three of the compounds, TCA, BNZ, and PCE at the 95 % confidence level; and 3) Excess hydrogen peroxide, beyond the stoichiometric value, increased the reaction rate constant for all five compounds. The greatest increase was seen for DCB.

Assay of Peracid in the Presence of Excess Hydrogen Peroxide

2000 ◽  
Vol 33 (3) ◽  
pp. 479-488 ◽  
Author(s):  
W. H. Binder ◽  
F. M. Menger
Keyword(s):  
Hydrogen Peroxide ◽  
Excess Hydrogen

Abstract 265: Assessment Of Oxidative DNA Double Strand Break And Repair In Cardiomyocytes

2013 ◽  
Vol 113 (suppl_1) ◽  
Author(s):  
Bo Ye ◽  
Ning Hou ◽  
Lu Xiao ◽  
Haodong Xu ◽  
Faqian Li
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Dna Damage ◽  
Dna Repair ◽  
Stress Responses ◽  
Strand Break ◽  
Neonatal Rat ◽  
Rat Cardiomyocytes ◽  
Repair Protein ◽  
Dna Repair Protein

Backgrounds: DNA damage occurs in cardiomyocytes during normal cellular metabolism and is significantly increased under cardiac stresses. How cardiomyocytes repair their DNA damage, especially DNA double strand breaks (DSBs), remains undetermined. We assessed DSBs caused by oxidative stress. More importantly, we investigated the spatiotemporal dynamics of DNA repair protein assembly/disassembly in DNA damage sites. Methods: Cultured neonatal rat cardiomyocytes were treated with different doses of hydrogen peroxide (H2O2) for 30 minutes to assess DNA damage response (DDR). To investigate the dynamics of DDR, cells were treated with 200 uM H2O2 and followed up to 72 hours. DSBs were evaluated by counting DNA damage foci after staining with antibody against histone H2AX phosphorylation at serine 139 (g-H2AX). The dynamics and posttranslational modification of DNA repair proteins were determined by Western blotting, immunolabeling, and confocal microscopy. Result: g-H2AX was proportionally increased to H2O2 dosage. Discrete nuclear g-H2AX foci were seen 30 minutes after hydrogen peroxide treatment with 50 uM, but became pannuclear when H2O2 was above 400 uM. At 200 uM of hydrogen peroxide, g-H2AX started to increase at 15 minutes and reached to highest levels at 60 minutes with up to 70 nuclear foci, started to decline at 2 hours, and returned to basal levels at 24 hours. DDR transducer kinase, ataxia telangiectasia mutated (ATM) was activated at 5 minutes with increased phosphorylation at serine 1981 (pATM) which started to decrease at 24 hours, but remained elevated up to 48 hours. Another DDR transducer kinase, ATM and Rad3-related (ATR) showed a biphasic activation at 30 minutes and 8 hours. ATM and ATR colocalized with g-H2AX. DNA damage mediator proteins such as MRN complex and p53BP1 were also recruited to sites of DNA damage at g-H2AX foci. Conclusions: DSBs and their repair have emerged as a new frontier of stress responses. Newly developed methods for studying g-H2AX and DNA repair protein dynamics can be explored to investigate DDR to oxidative stress in cardiomyocytes.

scholarly journals Distinctive adaptive response to repeated exposure to hydrogen peroxide associated with upregulation of DNA repair genes and cell cycle arrest

Redox Biology ◽  
2016 ◽  
Vol 9 ◽  
pp. 124-133 ◽  
Author(s):  
Gloria A. Santa-Gonzalez ◽  
Andrea Gomez-Molina ◽  
Mauricio Arcos-Burgos ◽  
Joel N. Meyer ◽  
Mauricio Camargo
Keyword(s):  
Cell Cycle ◽  
Hydrogen Peroxide ◽  
Dna Repair ◽  
Cell Cycle Arrest ◽  
Adaptive Response ◽  
Repeated Exposure ◽  
Dna Repair Genes ◽  
Cycle Arrest ◽  
Repair Genes
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