Prenatal exposure to bisphenol‐A altered miRNA‐224 and protein expression of aromatase in ovarian granulosa cells concomitant with elevated serum estradiol levels in F 1 adult offspring

2019 ◽  
Vol 33 (6) ◽  
Author(s):  
Christy Lite ◽  
Sheik S. S. J. Ahmed ◽  
Winkins Santosh ◽  
Barathi Seetharaman
Keyword(s):  
Bisphenol A ◽  
Protein Expression ◽  
Prenatal Exposure ◽  
Granulosa Cells ◽  
Elevated Serum ◽  
Adult Offspring ◽  
Serum Estradiol ◽  
Ovarian Granulosa Cells ◽  
Estradiol Levels

scholarly journals MicroRNA-204-5p regulates apoptosis by targeting Bcl2 in rat ovarian granulosa cells exposed to cadmium†

2020 ◽  
Vol 103 (3) ◽  
pp. 608-619
Author(s):  
Ping Zhong ◽  
Jin Liu ◽  
Hong Li ◽  
Senbin Lin ◽  
Lingfeng Zeng ◽  
...  
Keyword(s):  
Protein Expression ◽  
Granulosa Cells ◽  
B Cell Lymphoma ◽  
Experimental Conditions ◽  
Expression Levels ◽  
Ovarian Granulosa Cells ◽  
Mrna And Protein Expression ◽  
Induced Apoptosis ◽  
Apoptosis Regulation

Abstract This study aimed to investigate whether cadmium (Cd) cytotoxicity in rat ovarian granulosa cells (OGCs) is mediated through apoptosis or autophagy and to determine the role of microRNAs (miRNAs) in Cd cytotoxicity. To test this hypothesis, rat OGCs were exposed to 0, 10, and 20 μM CdCl2 in vitro. As the Cd concentration increased, OGC apoptosis increased. In addition, Cd promoted apoptosis by decreasing the mRNA and protein expression levels of inhibition of B-cell lymphoma 2 (Bcl2). However, under our experimental conditions, no autophagic changes in rat OGCs were observed, and the mRNA and protein expression levels of the autophagic markers microtubule-associated protein 1 light chain 3 alpha (Map1lc3b) and Beclin1 (Becn1) were not changed. Microarray chip analysis, miRNA screening, and bioinformatics approaches were used to further explore the roles of apoptosis regulation-related miRNAs. In total, 19 miRNAs putatively related to Cd-induced apoptosis in rat OGCs were identified. Notably, miR-204-5p, which may target Bcl2, was identified. Then, rat OGCs were cultured in vitro and used to construct the miR-204-5p-knockdown cell line LV2-short hairpin RNA (shRNA). LV2-shRNA cells were exposed to 20 μM Cd for 12 h, and the mRNA and protein expression levels of Bcl2 were increased. Our findings suggest that Cd is cytotoxic to rat OGCs, and mitochondrial apoptosis rather than autophagy mediates Cd-induced damage to OGCs. Cd also affects apoptosis-related miRNAs, and the underlying apoptotic mechanism may involve the Bcl2 gene.

Local effect of bisphenol A on the estradiol synthesis of ovarian granulosa cells from PCOS

2016 ◽  
Vol 33 (1) ◽  
pp. 21-25 ◽  
Author(s):  
Yuan Wang ◽  
Qinling Zhu ◽  
Xuan Dang ◽  
Yaqiong He ◽  
Xiaoxue Li ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Granulosa Cells ◽  
Local Effect ◽  
Ovarian Granulosa Cells ◽  
Estradiol Synthesis

The interference effects of bisphenol A on the synthesis of steroid hormones in human ovarian granulosa cells

2020 ◽  
Author(s):  
Junxia Shi ◽  
Chenyu Liu ◽  
Mingyue Chen ◽  
Jinhui Yan ◽  
Chonggang Wang ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Steroid Hormones ◽  
Granulosa Cells ◽  
Interference Effects ◽  
Ovarian Granulosa Cells

Melatonin mitigates bisphenol A-induced estradiol production and proliferation by porcine ovarian granulosa cells in vitro

2018 ◽  
Vol 192 ◽  
pp. 91-98 ◽  
Author(s):  
Guoyun Wu ◽  
Dan Song ◽  
Quanwei Wei ◽  
Jun Xing ◽  
Xiaoli Shi ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Granulosa Cells ◽  
Ovarian Granulosa Cells

Obesity and NonAdherence Correlate with Elevated Serum Estradiol Levels in Postmenopausal Women Receiving Adjuvant Aromatase Inhibitor Therapy

2014 ◽  
Vol 20 (5) ◽  
pp. 553-554 ◽  
Author(s):  
David A. Decker ◽  
Robert B. Reynolds ◽  
David C Molthrop ◽  
Elizabeth Griffith ◽  
Tiffany Encarnacion ◽  
...  
Keyword(s):  
Aromatase Inhibitor ◽  
Postmenopausal Women ◽  
Elevated Serum ◽  
Inhibitor Therapy ◽  
Serum Estradiol ◽  
Aromatase Inhibitor Therapy ◽  
Estradiol Levels
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