Amyloid β induces NLRP3 inflammasome activation in retinal pigment epithelial cells via NADPH oxidase- and mitochondria-dependent ROS production

2016 ◽  
Vol 31 (6) ◽  
pp. e21887 ◽  
Author(s):  
Ke Wang ◽  
Yong Yao ◽  
Xue Zhu ◽  
Kai Zhang ◽  
Fanfan Zhou ◽  
...  
Keyword(s):  
Nlrp3 Inflammasome ◽  
Retinal Pigment Epithelial ◽  
Retinal Pigment ◽  
Amyloid Β ◽  
Retinal Pigment Epithelial Cells ◽  
Inflammasome Activation ◽  
Ros Production ◽  
Pigment Epithelial ◽  
Nlrp3 Inflammasome Activation ◽  
Pigment Epithelial Cells

scholarly journals Cyanidin-3-glucoside Alleviates 4-Hydroxyhexenal-Induced NLRP3 Inflammasome Activation via JNK-c-Jun/AP-1 Pathway in Human Retinal Pigment Epithelial Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Xiaolu Jin ◽  
Chengtao Wang ◽  
Wei Wu ◽  
Tingting Liu ◽  
Baoping Ji ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Nlrp3 Inflammasome ◽  
Retinal Pigment Epithelial ◽  
Retinal Pigment ◽  
Retinal Pigment Epithelial Cells ◽  
Inflammasome Activation ◽  
Protective Effects ◽  
Pigment Epithelial ◽  
Nlrp3 Inflammasome Activation ◽  
Pigment Epithelial Cells

Recently, the NLRP3 inflammasome activation in the eyes has been known to be associated with the pathogenesis of age-related macular degeneration. The aim of this study was to investigate the protective effects of cyanidin-3-glucoside (C3G), an important anthocyanin with great potential for preventing eye diseases, against 4-hydroxyhexenal- (HHE-) induced inflammatory damages in human retinal pigment epithelial cells, ARPE-19. We noticed that C3G pretreatment to the ARPE-19 cells rescued HHE-induced antiproliferative effects. Cell apoptosis ratio induced by HHE was also decreased by C3G, measured by flow cytometry. The activation of NLRP3 inflammasome induced by HHE was found with increases of caspase-1 activity, proinflammatory cytokine releases (IL-1β and IL-18), and NLRP3 inflammasome-related gene expressions (NLRP3, IL-1β, IL-18, and caspase-1). The C3G showed potent inhibitive effects on these NLRP3 inflammasome activation hallmarks induced by HHE. Moreover, we noticed that the C3G’s pretreatment leads to a delayed and a decreased JNK activation in HHE-challenged ARPE-19 cells. Finally, using a luciferase reporter gene assay system, we demonstrated that HHE-induced activation protein- (AP-) 1 transcription activity was abolished by C3G pretreatment in a dose-dependent manner. Taken together, these data showed that HHE leads to inflammatory damages to ARPE-19 cells while C3G has great protective effects, highlighting future potential applications of C3G against AMD-associated inflammation.

scholarly journals Effects of concentration of amyloid β (Aβ) on viability of cultured retinal pigment epithelial cells

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Naonori Masuda ◽  
Hiroki Tsujinaka ◽  
Hiromasa Hirai ◽  
Mariko Yamashita ◽  
Tetsuo Ueda ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Retinal Pigment Epithelial ◽  
Retinal Pigment ◽  
Amyloid Β ◽  
Retinal Pigment Epithelial Cells ◽  
Pigment Epithelial ◽  
Pigment Epithelial Cells
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