scholarly journals In vivo cytochrome P450 activity alterations in diabetic nonalcoholic steatohepatitis mice

2016 ◽  
Vol 31 (2) ◽  
pp. e21840 ◽  
Author(s):  
Hui Li ◽  
John D. Clarke ◽  
Anika L. Dzierlenga ◽  
John Bear ◽  
Michael J. Goedken ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Nonalcoholic Steatohepatitis ◽  
Cytochrome P450 Activity ◽  
P450 Activity

scholarly journals Pediatric Cytochrome P450 Activity Alterations in Nonalcoholic Steatohepatitis

2017 ◽  
Vol 45 (12) ◽  
pp. 1317-1325 ◽  
Author(s):  
Hui Li ◽  
Mark J. Canet ◽  
John D. Clarke ◽  
Dean Billheimer ◽  
Stavra A. Xanthakos ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Nonalcoholic Steatohepatitis ◽  
Cytochrome P450 Activity ◽  
P450 Activity

Interspecies differences in the cytochrome P450 activity of hepatocytes exposed to PLGA and silica nanoparticles: an in vitro and in vivo investigation

Nanoscale ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 5171-5181 ◽  
Author(s):  
Raphaël Cornu ◽  
Nathalie Rougier ◽  
Yann Pellequer ◽  
Alf Lamprecht ◽  
Paul Hamon ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Silica Nanoparticles ◽  
Interspecies Differences ◽  
Hepatic Cells ◽  
Cytochrome P450 Activity ◽  
Set Up ◽  
P450 Activity

This study emphasizes the interest to set up relevant in vitro models using human hepatic cells to evaluate the hepatotoxicity of nanomedicines.

scholarly journals Rational engineering of photosynthetic electron flux enhances light-powered cytochrome P450 activity

2018 ◽  
Vol 3 (1) ◽  
Author(s):  
Adokiye Berepiki ◽  
John R Gittins ◽  
C Mark Moore ◽  
Thomas S Bibby
Keyword(s):  
Cytochrome P450 ◽  
Electron Flow ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Experimental System ◽  
Type I ◽  
Photosynthetic Electron Flow ◽  
Cytochrome P450 Activity ◽  
The Impact ◽  
P450 Activity

AbstractIn this study, we exploited a modified photosynthetic electron transfer chain (PET) in the model cyanobacterium Synechococcus PCC 7002, where electrons derived from water-splitting are used to power reactions catalyzed by a heterologous cytochrome P450 (CYP1A1). A simple in vivo fluorescent assay for CYP1A1 activity was employed to determine the impact of rationally engineering of photosynthetic electron flow. This showed that knocking out a subunit of the type I NADH dehydrogenase complex (NDH-1), suggested to be involved in cyclic photosynthetic electron flow (ΔndhD2), can double the activity of CYP1A1, with a concomitant increase in the flux of electrons from photosynthesis. This also resulted in an increase in cellular adenosine triphosphate (ATP) and the ATP/nicotinamide adenine dinucleotide phosphate (NADPH) ratio, suggesting that expression of a heterologous electron sink in photosynthetic organisms can be used to modify the bioenergetic landscape of the cell. We therefore demonstrate that CYP1A1 is limited by electron supply and that photosynthesis can be re-engineered to increase heterologous P450 activity for the production of high-value bioproducts. The increase in cellular ATP achieved could be harnessed to support metabolically demanding heterologous processes. Furthermore, this experimental system could provide valuable insights into the mechanisms of photosynthesis.

Effect of vanillin and ethyl vanillin on cytochrome P450 activity in vitro and in vivo

2012 ◽  
Vol 50 (6) ◽  
pp. 1897-1901 ◽  
Author(s):  
Xiao-min Chen ◽  
Min Wei ◽  
Hai-mou Zhang ◽  
Cheng-hao Luo ◽  
Yi-kun Chen ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Ethyl Vanillin ◽  
Cytochrome P450 Activity ◽  
P450 Activity

scholarly journals Cytochrome P450 enzymes inhibitor in the control of Digitaria insularis

2021 ◽  
Vol 45 ◽  
Author(s):  
Gledson Soares de Carvalho ◽  
Jessica Ferreira Lourenço Leal ◽  
Amanda dos Santos Souza ◽  
Francisco Freire de Oliveira Junior ◽  
Ana Claudia Langaro ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Block Design ◽  
Antagonistic Effect ◽  
Cytochrome P450 Enzymes ◽  
Cytochrome P450 Activity ◽  
Randomized Block Design ◽  
Untreated Check ◽  
Herbicide Mixtures ◽  
P450 Activity

ABSTRACT Herbicide interactions can be synergic, additive, or antagonist when mixed in the spray tank. A good example is an association between 2,4-D and graminicides. One hypothesis is that 2,4-D contributes to increasing the Cytochrome P450 activity, which may be one of the causes of antagonism. This study aimed to investigate the use of CYP450 enzymes inhibitor associated with the herbicide mixtures 2,4-D and ACCase inhibitors in vivo on the control of Digitaria insularis. The experiment was performed using a randomized block design in a factorial scheme of 6x2 with four replications. Factor A consisted of untreated check, 2,4-D (1005 g a.e ha-1), clethodim (192 g a.i ha-1) and haloxyfop (62.4 g a.i ha-1), 2,4-D + clethodim (1005 g a.e ha-1+192 g a.i ha-1), and 2,4-D + haloxyfop (1005 g a.e ha-1 +62.4 g a.i ha-1). Factor B represented the presence or absence of malathion (1000 g ha-1) applied two hours before applying the herbicides. A physicochemical test was performed to verify the compatibility of the herbicides in the tank. Malathion application performed two hours before applying the herbicide mixtures (2,4-D and clethodim/haloxyfop) did not provide adequate control of sourgrass, suggesting that CYP450 enzymes inhibited by malathion are not involved in the antagonistic effect between 2,4-D and both graminicides in the management of sourgrass. The 2,4-D + haloxyfop in tank mix demonstrated less efficacy in controlling sourgrass than 2,4-D + clethodim, but both mixtures were incompatible in the tank mix, which may be associated with reduced efficacy in sourgrass management.

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