scholarly journals Role of Calcium and Calpain in the Downregulation of Voltage-Gated Sodium Channel Expression by the Pyrethroid Pesticide Deltamethrin

2014 ◽  
Vol 29 (3) ◽  
pp. 129-134 ◽  
Author(s):  
Jason P. Magby ◽  
Jason R. Richardson
Keyword(s):  
Sodium Channel ◽  
Voltage Gated Sodium Channel ◽  
Voltage Gated ◽  
Pyrethroid Pesticide ◽  
Channel Expression

Abstract 17384: Cardiac-Specific Overexpression of Human Mutant Desmoplakin in Mice Disrupts Cardiac Voltage-Gated Sodium Channel Expression

Circulation ◽  
2018 ◽  
Vol 138 (Suppl_1) ◽  
Author(s):  
Subat Turdi ◽  
Jeffrey A Towbin
Keyword(s):  
Sodium Channel ◽  
Fatty Infiltration ◽  
Electrical Coupling ◽  
Cx43 Expression ◽  
Voltage Gated Sodium Channel ◽  
Voltage Gated ◽  
Intercalated Discs ◽  
Channel Expression ◽  
Life Threatening ◽  
And Function

Introduction: Arrhythmogenic cardiomyopathy (AC) is characterized by bi-ventricular dilation, fibro-fatty infiltration and life-threatening arrhythmias. Disruptions in cardiac voltage-gated sodium channel (Nav1.5) expression and function are known to cause arrhythmias. We have demonstrated that cardiac-specific overexpression of human mutant desmoplakin (DSP, Tg-R2834H) in mice leads to AC. However, whether mutant DSP expression in the heart affects the Nav1.5 distribution and function are unknown Hypothesis: Here, we tested whether Nav1.5 localization and expression are altered in the R2834H-Tg mouse hearts. Methods: Primary cardiomyocytes and frozen myocardial sections from non-transgenic (NTg), wild-type DSP (Tg-DSP) and Tg-R2834H mice were used for immunofluorescence studies to assess subcellular localization of DSP, desmin, Nav1.5, Cx43, plakoglobin and β-catenin. Western blot and qPCR were used for quantitative analysis. Results: Double staining of cardiomyocytes from NTg mice with DSP and Nav1.5 revealed that Nav1.5 was colocalized with DSP at the intercalated discs (IDs). In contrast, Tg-R2834H cardiomyocytes exhibited marked increase of mutant DSP expression at the IDs concomitant with a reduction in Nav1.5 immunoreactive signals. Tg-R2834H cardiomyocytes also revealed an aberration of DSP and desmin colocalizations at the IDs. There were not obvious differences in Cx43 expression between the genotypes, although the redistribution of Cx43 from the IDs to the sarcolemma was evident in Tg-R2834H cardiomyocytes. qPCR results correlated with reduced Nav1.5 mRNA expression in the Tg-R2834H mouse hearts. Conclusions: Defective DSP protein expression in the heart disrupts Nav1.5 localization and expression, implying an interaction between DSP and Nav1.5 to orchestrate normal mechanical and electrical coupling. Further electrophysiology studies to assess whole-cell Na + currents in these cardiomyocytes will provide insight into DSP and Nav1.5 interaction.

Voltage-gated sodium channel expression in rat and human epidermal keratinocytes: Evidence for a role in pain

Pain ◽  
2008 ◽  
Vol 139 (1) ◽  
pp. 90-105 ◽  
Author(s):  
Peng Zhao ◽  
Travis P. Barr ◽  
Quanzhi Hou ◽  
Sulayman D. Dib-Hajj ◽  
Joel A. Black ◽  
...  
Keyword(s):  
Sodium Channel ◽  
Epidermal Keratinocytes ◽  
Human Epidermal Keratinocytes ◽  
Voltage Gated Sodium Channel ◽  
Voltage Gated ◽  
Channel Expression

scholarly journals Functional role of the C-terminus of voltage-gated sodium channel Nav1.8

FEBS Letters ◽  
2004 ◽  
Vol 572 (1-3) ◽  
pp. 256-260 ◽  
Author(s):  
Jin-Sung Choi ◽  
Lynda Tyrrell ◽  
Stephen G Waxman ◽  
Sulayman D Dib-Hajj
Keyword(s):  
Sodium Channel ◽  
Functional Role ◽  
Voltage Gated Sodium Channel ◽  
Voltage Gated ◽  
C Terminus

scholarly journals Voltage-Gated Sodium Channel Expression and Potentiation of Human Breast Cancer Metastasis

2005 ◽  
Vol 11 (15) ◽  
pp. 5381-5389 ◽  
Author(s):  
Scott P. Fraser ◽  
James K.J. Diss ◽  
Athina-Myrto Chioni ◽  
Maria E. Mycielska ◽  
Huiyan Pan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Sodium Channel ◽  
Human Breast Cancer ◽  
Cancer Metastasis ◽  
Breast Cancer Metastasis ◽  
Human Breast ◽  
Voltage Gated Sodium Channel ◽  
Voltage Gated ◽  
Channel Expression
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