Murine Tissue‐Resident PDGFRα+ Fibro‐Adipogenic Progenitors Spontaneously Acquire Osteogenic Phenotype in an Altered Inflammatory Environment

2020 ◽  
Vol 35 (8) ◽  
pp. 1525-1534 ◽  
Author(s):  
Christine Eisner ◽  
Michael Cummings ◽  
Gabrielle Johnston ◽  
Lin Wei Tung ◽  
Elena Groppa ◽  
...  
Keyword(s):  
Murine Tissue

Aspirin Inhibits Tumor Necrosis Factor-α Gene Expression in Murine Tissue Macrophages

1997 ◽  
Vol 52 (3) ◽  
pp. 421-429 ◽  
Author(s):  
Rodney E. Shackelford ◽  
Paul B. Alford ◽  
Yan Xue ◽  
Sheau-Fung Thai ◽  
Dolph O. Adams ◽  
...  
Keyword(s):  
Gene Expression ◽  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Murine Tissue ◽  
Factor Α ◽  
Necrosis Factor

scholarly journals Host Rather than Graft Origin of Matrigel-Induced Adipose Tissue in the Murine Tissue-Engineering Chamber

2007 ◽  
Vol 13 (9) ◽  
pp. 2291-2300 ◽  
Author(s):  
Filip Stillaert ◽  
Michael Findlay ◽  
Jason Palmer ◽  
Rejhan Idrizi ◽  
Shirley Cheang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Adipose Tissue ◽  
Murine Tissue

scholarly journals Collagenase 2 (MMP-8) Expression in Murine Tissue-remodeling Processes

1998 ◽  
Vol 273 (37) ◽  
pp. 23959-23968 ◽  
Author(s):  
Milagros Balbı́n ◽  
Antonio Fueyo ◽  
Vera Knäuper ◽  
Alberto M. Pendás ◽  
José M. López ◽  
...  
Keyword(s):  
Tissue Remodeling ◽  
Murine Tissue

scholarly journals Purification and properties of sialoadhesin, a sialic acid-binding receptor of murine tissue macrophages.

1991 ◽  
Vol 10 (7) ◽  
pp. 1661-1669 ◽  
Author(s):  
P.R. Crocker ◽  
S. Kelm ◽  
C. Dubois ◽  
B. Martin ◽  
A.S. McWilliam ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Purification And Properties ◽  
Sialic Acid Binding ◽  
Murine Tissue

scholarly journals Deep Learning Identifies Cardiomyocyte Nuclei in Murine Tissue with High Precision

2020 ◽  
Author(s):  
Shah R. Ali ◽  
Dan Nguyen ◽  
Brandon Wang ◽  
Steven Jiang ◽  
Hesham A. Sadek
Keyword(s):  
Deep Learning ◽  
Cell Types ◽  
Label Cell ◽  
Biological Research ◽  
Mouse Heart ◽  
Specific Cell ◽  
Label Free ◽  
Structural Protein ◽  
Mammalian Tissues ◽  
Murine Tissue

ABSTRACTProper identification and annotation of cells in mammalian tissues is of paramount importance to biological research. Various approaches are currently used to identify and label cell types of interest in complex tissues. In this report, we generated an artificial intelligence (AI) deep learning model that uses image segmentation to predict cardiomyocyte nuclei in mouse heart sections without a specific cardiomyocyte nuclear label. This tool can annotate cardiomyocytes highly sensitively and specifically (AUC 0.94) using only cardiomyocyte structural protein immunostaining and a global nuclear stain. We speculate that our method is generalizable to other tissues to annotate specific cell types and organelles in a label-free way.

scholarly journals Genetic polymorphism of murine tissue plasminogen activator associated with antiphospholipid syndrome

1999 ◽  
Vol 1 (2) ◽  
pp. 130-136 ◽  
Author(s):  
J Shirai ◽  
A Ida ◽  
Y Jiang ◽  
R Sanokawa-Akakura ◽  
Y Miura ◽  
...  
Keyword(s):  
Genetic Polymorphism ◽  
Plasminogen Activator ◽  
Tissue Plasminogen Activator ◽  
Antiphospholipid Syndrome ◽  
Tissue Plasminogen ◽  
Murine Tissue

Propagation based differential phase contrast imaging and tomography of murine tissue with a laser plasma x-ray source

2007 ◽  
Vol 91 (17) ◽  
pp. 173901 ◽  
Author(s):  
Christopher M. Laperle ◽  
Philip Wintermeyer ◽  
Jack R. Wands ◽  
Daxin Shi ◽  
Mark A. Anastasio ◽  
...  
Keyword(s):  
Laser Plasma ◽  
Phase Contrast ◽  
Phase Contrast Imaging ◽  
Contrast Imaging ◽  
X Ray ◽  
Differential Phase ◽  
Murine Tissue ◽  
Differential Phase Contrast

scholarly journals Discovery of Glucocorticoid Receptor-β in Mice with a Role in Metabolism

2010 ◽  
Vol 24 (9) ◽  
pp. 1715-1727 ◽  
Author(s):  
Terry D. Hinds ◽  
Sadeesh Ramakrishnan ◽  
Harrison A. Cash ◽  
Lance A. Stechschulte ◽  
Garrett Heinrich ◽  
...  
Keyword(s):  
Glucocorticoid Receptor ◽  
Hairpin Rna ◽  
Culture Cells ◽  
Physiological Processes ◽  
Inflammatory Signals ◽  
Tissue Culture Cells ◽  
Rigorous Testing ◽  
Murine Tissue ◽  
Exon 9 ◽  
Rna Knockdown

Abstract Glucocorticoid hormones control diverse physiological processes, including metabolism and immunity, by activating the major glucocorticoid receptor (GR) isoform, GRα. However, humans express an alternative isoform, human (h)GRβ, that acts as an inhibitor of hGRα to produce a state of glucocorticoid resistance. Indeed, evidence exists that hGRβ contributes to many diseases and resistance to glucocorticoid hormone therapy. However, rigorous testing of the GRβ contribution has not been possible, because rodents, especially mice, are not thought to express the β-isoform. Here, we report expression of GRβ mRNA and protein in the mouse. The mGRβ isoform arises from a distinct alternative splicing mechanism utilizing intron 8, rather than exon 9 as in humans. The splicing event produces a form of β that is similar in structure and functionality to hGRβ. Mouse (m)GRβ has a degenerate C-terminal region that is the same size as hGRβ. Using a variety of newly developed tools, such as a mGRβ-specific antibody and constructs for overexpression and short hairpin RNA knockdown, we demonstrate that mGRβ cannot bind dexamethasone agonist, is inhibitory of mGRα, and is up-regulated by inflammatory signals. These properties are the same as reported for hGRβ. Additionally, novel data is presented that mGRβ is involved in metabolism. When murine tissue culture cells are treated with insulin, no effect on mGRα expression was observed, but GRβ was elevated. In mice subjected to fasting-refeeding, a large increase of GRβ was seen in the liver, whereas mGRα was unchanged. This work uncovers the much-needed rodent model of GRβ for investigations of physiology and disease.

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