scholarly journals Bone sialoprotein deficiency impairs osteoclastogenesis and mineral resorption in vitro

2010 ◽  
Vol 25 (12) ◽  
pp. 2669-2679 ◽  
Author(s):  
Maya Boudiffa ◽  
Ndéye Marième Wade-Gueye ◽  
Alain Guignandon ◽  
Arnaud Vanden-Bossche ◽  
Odile Sabido ◽  
...  
Keyword(s):  
Bone Sialoprotein ◽  
Mineral Resorption

scholarly journals Bone Sialoprotein Deficiency Impairs Osteoclastogenesis and Mineral Resorption In Vitro

2020 ◽  
Vol 35 (8) ◽  
pp. 1617-1617
Author(s):  
Maya Boudiffa ◽  
Ndéye Marième Wade‐Gueye ◽  
Alain Guignandon ◽  
Arnaud Vanden‐Bossche ◽  
Odile Sabido ◽  
...  
Keyword(s):  
Bone Sialoprotein ◽  
Mineral Resorption

Fabrication and Bioactivity of Porous Titanium Implant

2007 ◽  
Vol 342-343 ◽  
pp. 613-616
Author(s):  
Xia Lu ◽  
Li Ang Xing ◽  
Pei Zhi Wang ◽  
Jun Fu
Keyword(s):  
Alkaline Phosphatase ◽  
Titanium Implant ◽  
Study Groups ◽  
Porous Titanium ◽  
Bone Sialoprotein ◽  
Control Group ◽  
Ha Coating ◽  
Titanium Material ◽  
Polymerase Chain

The aim of this study is to fabricate an implant framework for tissue engineering by sintering titanium beads and coating with hydroxyapatite and test its biocompatibility and bioactivity in vitro. The porous titanium with and without hydroxyapatite coating were involved in study groups. Osteoblastic proliferation, activity of alkaline phosphatase, mRNA of osteocalcin and bone sialoprotein were detected by MTT-assay, ALP test and real-time polymerase chain reaction respectively. The results indicated that the porous titanium material with/without HA coating could promote osteoblastic proliferation significantly contrast to the control group. However, only porous titanium with HA coating increased alkaline phosphatase, osteocalcin and bone sialoprotein gene expression apparently and had statistically differences with the other two groups. Abstract no. is TE-Po-044

scholarly journals Fetal bovine bone cells synthesize bone-specific matrix proteins.

1984 ◽  
Vol 99 (2) ◽  
pp. 607-614 ◽  
Author(s):  
S W Whitson ◽  
W Harrison ◽  
M K Dunlap ◽  
D E Bowers ◽  
L W Fisher ◽  
...  
Keyword(s):  
Type I Collagen ◽  
Bone Cells ◽  
Bone Sialoprotein ◽  
Cell System ◽  
Matrix Proteins ◽  
Type I ◽  
Bovine Bone ◽  
Isolated Cells ◽  
Noncollagenous Proteins

We isolated cells from both calvaria and the outer cortices of long bones from 3- to 5-mo bovine fetuses. The cells were identified as functional osteoblasts by indirect immunofluorescence using antibodies against three bone-specific, noncollagenous matrix proteins (osteonectin, the bone proteoglycan, and the bone sialoprotein) and against type 1 collagen. In separate experiments, confluent cultures of the cells were radiolabeled and shown to synthesize and secrete osteonectin, the bone proteoglycan and the bone sialoprotein by immunoprecipitation and fluorography of SDS polyacrylamide gels. Analysis of the radiolabeled collagens synthesized by the cultures showed that they produced predominantly (approximately 94%) type I collagen, with small amounts of types III and V collagens. In agreement with previous investigators who have employed the rodent bone cell system, we confirmed in bovine bone cells that (a) there was a typical cyclic AMP response to parathyroid hormone, (b) freshly isolated cells possessed high levels of alkaline phosphatase, which diminished during culture but returned to normal levels in mineralizing cultures, and (c) cells grown in the presence of ascorbic acid and beta-glycerophosphate rapidly produced and mineralized an extracellular matrix containing largely type I collagen. These results show that antibodies directed against bone-specific, noncollagenous proteins can be used to clearly identify bone cells in vitro.

Bone Sialoprotein Promotes Tumor Cell Migration in Both In Vitro and In Vivo Models

2003 ◽  
Vol 44 (1) ◽  
pp. 279-284 ◽  
Author(s):  
J. Chen ◽  
J. A. Rodriguez ◽  
B. Barnett ◽  
N. Hashimoto ◽  
J. Tang ◽  
...  
Keyword(s):  
Cell Migration ◽  
Tumor Cell ◽  
Bone Sialoprotein ◽  
In Vivo Models ◽  
Tumor Cell Migration

scholarly journals In vitro bone sialoprotein-I expression in combined gingival stromal cells and platelet rich fibrin during osteogenic differentiation

2019 ◽  
Vol 17 (12) ◽  
pp. 2341 ◽  
Author(s):  
Alexander Patera Nugraha ◽  
Ida Bagus Narmada ◽  
Diah Savitri Ernawati ◽  
Aristika Dinaryanti ◽  
Eryk Hendrianto ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Stromal Cells ◽  
Bone Sialoprotein ◽  
Platelet Rich Fibrin
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