scholarly journals SDF-1/CXCR4 Axis in Tie2-Lineage Cells Including Endothelial Progenitor Cells Contributes to Bone Fracture Healing

2014 ◽  
Vol 30 (1) ◽  
pp. 95-105 ◽  
Author(s):  
Yohei Kawakami ◽  
Masaaki Ii ◽  
Tomoyuki Matsumoto ◽  
Ryosuke Kuroda ◽  
Tomoya Kuroda ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Bone Fracture ◽  
Bone Fracture Healing ◽  
Endothelial Progenitor ◽  
Cxcr4 Axis

scholarly journals Conditioned media from endothelial progenitor cells cultured in simulated microgravity promote angiogenesis and bone fracture healing

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Lingchi Kong ◽  
Yan Wang ◽  
Haixing Wang ◽  
Qi Pan ◽  
Rongtai Zuo ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Bone Fracture ◽  
Mechanical Test ◽  
Human Peripheral Blood ◽  
Mapk Signaling ◽  
Fracture Repair ◽  
Conditioned Media ◽  
Endothelial Progenitor

Abstract Background Paracrine signaling from endothelial progenitor cells (EPCs) is beneficial for angiogenesis and thus promotes tissue regeneration. Microgravity (MG) environment is found to facilitate the functional potentials of various stem or progenitor cells. The present study aimed to elucidate the effects of MG on pro-angiogenic properties and fracture repair capacities of conditioned media (CM) from EPCs. Methods Human peripheral blood-derived EPCs were cultured under MG or normal gravity (NG) followed by analysis for angiogenic gene expression. Furthermore, the serum-free CM under MG (MG-CM) or NG (NG-CM) were collected, and their pro-angiogenic properties were examined in human umbilical vein endothelial cells (HUVECs). In order to investigate the effects of MG-CM on fracture healing, they were injected into the fracture gaps of rat models, and radiography, histology, and mechanical test were performed to evaluate neovascularization and fracture healing outcomes. Results MG upregulated the expression of hypoxia-induced factor-1α (HIF-1α) and endothelial nitric oxide synthase (eNOS) and promoted NO release. Comparing to NG-CM, MG-CM significantly facilitated the proliferation, migration, and angiogenesis of HUVECs through NO-induced activation of FAK/Erk1/2-MAPK signaling pathway. In addition, MG-CM were verified to improve angiogenic activities in fracture area in a rat tibial fracture model, accelerate fracture healing, and well restore the biomechanical properties of fracture bone superior to NG-CM. Conclusion These findings provided insight into the use of MG bioreactor to enhance the angiogenic properties of EPCs’ paracrine signals via HIF-1α/eNOS/NO axis, and the administration of MG-CM favored bone fracture repair. Graphical abstract

Dynamic compression promotes proliferation and neovascular networks of endothelial progenitor cells in demineralized bone matrix scaffold seed

2012 ◽  
Vol 113 (4) ◽  
pp. 619-626 ◽  
Author(s):  
Zhan Kong ◽  
Jianjun Li ◽  
Qun Zhao ◽  
Zhendong Zhou ◽  
Xiangnan Yuan ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Demineralized Bone Matrix ◽  
Dynamic Compression ◽  
Bone Matrix ◽  
Tube Formation ◽  
Endothelial Progenitor ◽  
Demineralized Bone

Neovascularization is required for bone formation and successful fracture healing. In the process of neovascularization, endothelial progenitor cells (EPCs) play an important role and finish vascular repair through reendothelialization to promote successful fracture healing. In this study, we found that dynamic compression can promote the proliferation and capillary-like tube formation of EPCs in the demineralized bone matrix (DBM) scaffold seed. EPCs isolated from the bone marrow of rats have been cultured in DBM scaffolds before dynamic compression and then seeded in the DBM scaffolds under dynamic conditions. The cells/scaffold constructs were subjected to cyclic compression with 5% strain and at 1 Hz for 4 h/day for 7 consecutive days. By using MTT and real-time PCR, we found that dynamic compression can significantly induce the proliferation of EPCs in three-dimensional culture with an even distribution of cells onto DBM scaffolds. Both in vitro and in vivo, the tube formation assays in the scaffolds showed that the loaded EPCs formed significant tube-like structures. These findings suggest that dynamic compression promoted the vasculogenic activities of EPCs seeded in the scaffolds, which would benefit large bone defect tissue engineering.

scholarly journals Spleen recruits endothelial progenitor cells (EPCs) via SDF-1/CXCR4 axis

Heart ◽  
2011 ◽  
Vol 97 (Suppl 3) ◽  
pp. A86-A86 ◽  
Author(s):  
X. Zhao ◽  
D. Qian ◽  
N. Wu ◽  
Y. Yin ◽  
M. Song ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Endothelial Progenitor ◽  
Cxcr4 Axis

scholarly journals Spleen recruits endothelial progenitor cells (EPCs) via SDF-1/CXCR4 axis(*)

Heart ◽  
2011 ◽  
Vol 97 (Suppl 3) ◽  
pp. A92-A93
Author(s):  
X. Zhao ◽  
D. Qian ◽  
N. Wu ◽  
Y. Yin ◽  
M. Song ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Endothelial Progenitor ◽  
Cxcr4 Axis

Mobilization of endothelial progenitor cells in fracture healing and distraction osteogenesis

Bone ◽  
2008 ◽  
Vol 42 (5) ◽  
pp. 932-941 ◽  
Author(s):  
Dong Yeon Lee ◽  
Tae-Joon Cho ◽  
Jin A Kim ◽  
Hye Ran Lee ◽  
Won Joon Yoo ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Distraction Osteogenesis ◽  
Endothelial Progenitor
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