scholarly journals Brd4 inactivation increases adenoviral delivery of BMP2 for paracrine stimulation of osteogenic differentiation as a gene therapeutic concept to enhance bone healing

JBMR Plus ◽  
2021 ◽  
Author(s):  
Christopher R. Paradise ◽  
Rodolfo E. De La Vega ◽  
M. Lizeth Galvan ◽  
Margarita E. Carrasco ◽  
Roman Thaler ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Bone Healing ◽  
Therapeutic Concept ◽  
Adenoviral Delivery ◽  
Stimulation Of

scholarly journals Combination of Human Amniotic Fluid Derived-Mesenchymal Stem Cells and Nano-hydroxyapatite Scaffold Enhances Bone Regeneration

2019 ◽  
Vol 7 (17) ◽  
pp. 2739-2750 ◽  
Author(s):  
Eman E. A. Mohammed ◽  
Hanan Beherei ◽  
Mohamed El-Zawahry ◽  
Abdel Razik Farrag ◽  
Naglaa Kholoussi ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Bone Healing ◽  
Therapeutic Potential ◽  
Osteogenic Potential ◽  
3D Scaffold ◽  
Human Amniotic Fluid ◽  
Post Transplantation ◽  
Defect Site ◽  
Tibia Defect

BACKGROUND: Human amniotic fluid-derived stem cells (hAF-MSCs) have a high proliferative capacity and osteogenic differentiation potential in vitro. The combination of hAF-MSCs with three-dimensional (3D) scaffold has a promising therapeutic potential in bone tissue engineering and regenerative medicine. Selection of an appropriate scaffold material has a crucial role in a cell supporting and osteoinductivity to induce new bone formation in vivo. AIM: This study aimed to investigate and evaluate the osteogenic potential of the 2nd-trimester hAF-MSCs in combination with the 3D scaffold, 30% Nano-hydroxyapatite chitosan, as a therapeutic application for bone healing in the induced tibia defect in the rabbit. SUBJECT AND METHODS: hAF-MSCs proliferation and culture expansion was done in vitro, and osteogenic differentiation characterisation was performed by Alizarin Red staining after 14 & 28 days. Expression of the surface markers of hAF-MSCs was assessed using Flow Cytometer with the following fluorescein-labelled antibodies: CD34-PE, CD73-APC, CD90-FITC, and HLA-DR-FITC. Ten rabbits were used as an animal model with an induced defect in the tibia to evaluate the therapeutic potential of osteogenic differentiation of hAF-MSCs seeded on 3D scaffold, 30% Nano-hydroxyapatite chitosan. The osteogenic differentiated hAF-MSCs/scaffold composite system applied and fitted in the defect region and non-seeded scaffold was used as control. The histopathological investigation was performed at 2, 3, & 4 weak post-transplantation and scanning electron microscope (SEM) was assessed at 2 & 4 weeks post-transplantation to evaluate the bone healing potential in the rabbit tibia defect. RESULTS: Culture and expansion of 2nd-trimester hAF-MSCs presented high proliferative and osteogenic potential in vitro. Histopathological examination for the transplanted hAF-MSCs seeded on the 3D scaffold, 30% Nano-hydroxyapatite chitosan, demonstrated new bone formation in the defect site at 2 & 3 weeks post-transplantation as compared to the control (non-seeded scaffold). Interestingly, the scaffold accelerated the osteogenic differentiation of AF-MSCs and showed complete bone healing of the defect site as compared to the control (non-seeded scaffold) at 4 weeks post-transplantation. Furthermore, the SEM analysis confirmed these findings. CONCLUSION: The combination of the 2nd-trimester hAF-MSCs and 3D scaffold, 30% Nano-hydroxyapatite chitosan, have a therapeutic perspective for large bone defect and could be used effectively in bone tissue engineering and regenerative medicine.

Stimulation of osteogenic differentiation in bone marrow stromal cells via Wnt/β-catenin pathway by Qili Jiegu-containing serum

2018 ◽  
Vol 103 ◽  
pp. 1664-1668
Author(s):  
Han-Bing Song ◽  
Ying Jiang ◽  
Jia-Xing Liu ◽  
Ge-Qiang Wang ◽  
Da-Peng Zhang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Stimulation Of

scholarly journals PPARβ/δ accelerates bone regeneration in diabetic mellitus by enhancing AMPK/mTOR pathway-mediated autophagy

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Miao Chen ◽  
Dian Jing ◽  
Rui Ye ◽  
Jianru Yi ◽  
Zhihe Zhao
Keyword(s):  
Bone Regeneration ◽  
Osteogenic Differentiation ◽  
Bone Healing ◽  
High Glucose ◽  
Diabetic Rats ◽  
Diabetic Patients ◽  
Regulatory Effect ◽  
Compound C ◽  
High Glucose Condition ◽  
Glucose Condition

Abstract Background Diabetic patients are more vulnerable to skeletal complications. Peroxisome proliferators-activated receptor (PPAR) β/δ has a positive regulatory effect on bone turnover under physiologic glucose concentration; however, the regulatory effect in diabetes mellitus has not been investigated yet. Herein, we explored the effects of PPARβ/δ agonist on the regeneration of diabetic bone defects and the osteogenic differentiation of rat bone marrow mesenchymal stem cells (rBMSCs) under a pathological high-glucose condition. Methods We detected the effect of PPARβ/δ agonist on osteogenic differentiation of rBMSCs in vitro and investigated the bone healing process in diabetic rats after PPARβ/δ agonist treatment in vivo. RNA sequencing was performed to detect the differentially expressed genes and enriched pathways. Western blot was performed to detect the autophagy-related protein level. Laser confocal microscope (LSCM) and transmission electron microscope (TEM) were used to observe the formation of autophagosomes. Results Our results demonstrated that the activation of PPARβ/δ can improve the osteogenic differentiation of rBMSCs in high-glucose condition and promote the bone regeneration of calvarial defects in diabetic rats, while the inhibition of PPARβ/δ alleviated the osteogenic differentiation of rBMSCs. Mechanistically, the activation of PPARβ/δ up-regulates AMPK phosphorylation, yielding mTOR suppression and resulting in enhanced autophagy activity, which further promotes the osteogenic differentiation of rBMSCs in high-glucose condition. The addition of AMPK inhibitor Compound C or autophagy inhibitor 3-MA inhibited the osteogenesis of rBMSCs in high-glucose condition, suggesting that PPARβ/δ agonist promotes osteogenic differentiation of rBMSCs through AMPK/mTOR-regulated autophagy. Conclusion In conclusion, our study demonstrates the potential role of PPARβ/δ as a molecular target for the treatment of impaired bone quality and delayed bone healing in diabetic patients for the first time.

Dioxin Exposure Inhibits Osteogenic Differentiation and Impairs Bone Healing in a Rat Spine Fusion Model

2015 ◽  
Vol 15 (10) ◽  
pp. S135-S136
Author(s):  
Chawon Yun ◽  
Ryan Freshman ◽  
Danielle Chun ◽  
Sean M. Mitchell ◽  
Abhishek Kannan ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Bone Healing ◽  
Spine Fusion ◽  
Fusion Model ◽  
Dioxin Exposure

Experimental Stimulation of Bone Healing with Teriparatide: Histomorphometric and Microhardness Analysis in a Mouse Model of Closed Fracture

2011 ◽  
Vol 89 (2) ◽  
pp. 163-171 ◽  
Author(s):  
Barbara Mognetti ◽  
Silvia Marino ◽  
Alessandro Barberis ◽  
Anne-Sophie Bravo Martin ◽  
Yohann Bala ◽  
...  
Keyword(s):  
Mouse Model ◽  
Bone Healing ◽  
Closed Fracture ◽  
Stimulation Of
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