In vivo efficacy of 3D ‐printed elastin–gelatin–hyaluronic acid scaffolds for regeneration of nasal septal cartilage defects

2021 ◽  
Author(s):  
Abbas Shokri ◽  
Kousar Ramezani ◽  
Mohammad Reza Jamalpour ◽  
Chiman Mohammadi ◽  
Farshid Vahdatinia ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Septal Cartilage ◽  
Cartilage Defects ◽  
In Vivo Efficacy ◽  
3D Printed ◽  
Nasal Septal Cartilage

Tissue Engineered Human Septal Cartilage in a Murine Model

2008 ◽  
Vol 139 (2_suppl) ◽  
pp. P87-P88
Author(s):  
Angela Chang ◽  
Sage August ◽  
Barbara L Schumacher ◽  
Williams Gregory ◽  
Robert L Sah ◽  
...  
Keyword(s):  
Cell Viability ◽  
Murine Model ◽  
Three Dimensional ◽  
Alginate Beads ◽  
Septal Cartilage ◽  
Hematoxylin And Eosin ◽  
Matrix Production ◽  
Nasal Septal Cartilage

Problem Tissue engineering of human nasal septal cartilage represents an alternative technique for creating large quantities of autologous material for use in reconstructive surgery of the head and neck. Septal neocartilage constructs developed in vitro by the alginate method have demonstrated cartilaginous extracellular matrix production, but their biocompatibility and development in vivo remains largely unknown. Methods A murine model was used to examine the behavior of neocartilage constructs in vivo. Chondrocytes collected from donors undergoing septoplasty were expanded in monolayer and suspended in alginate beads for three-dimensional culture in media containing human serum and growth factors. After in vitro incubation for 5 weeks, the neocartilage constructs were implanted subcutaneously in the dorsum of athymic mice for 30 days (n=3). The mice were sacrificed and the constructs were explanted for assessment of cell viability, gross morphology, and histology. Results The mice survived and tolerated the implant well. Infection and extrusion were not observed. Neocartilage constructs maintained their general shape and size, and demonstrated cell viability after implantation. Explanted constructs were firm and opaque, sharing closer semblance to native septal tissue relative to the gelatinous, translucent pre-implant constructs. On hematoxylin and eosin staining, the explanted constructs exhibited distinct morphologies characteristic of native tissue, which were not observed in pre-implant constructs. Conclusion Neocartilage constructs are viable in an in vivo murine model. The morphologic and histologic features of explanted constructs more closely resemble native septal tissue when compared to pre-implant constructs. Significance Septal neocartilage constructs are biocompatible and demonstrate potential for in vivo maturation with eventual clinical application.

Trimethyl chitosan-hyaluronic acid nano-polyplexes for intravitreal VEGFR-2 siRNA delivery: Formulation and in vivo efficacy evaluation

2020 ◽  
Vol 26 ◽  
pp. 102181 ◽  
Author(s):  
Farkhondeh Chaharband ◽  
Narsis Daftarian ◽  
Mozhgan Rezaei Kanavi ◽  
Reyhaneh Varshochian ◽  
Maliheh Hajiramezanali ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Sirna Delivery ◽  
Efficacy Evaluation ◽  
In Vivo Efficacy ◽  
Trimethyl Chitosan

Will Tissue-Engineering Strategies Bring New Hope for the Reconstruction of Nasal Septal Cartilage?

2020 ◽  
Vol 15 (2) ◽  
pp. 144-154
Author(s):  
Zohreh Bagher ◽  
Negin Asgari ◽  
Parisa Bozorgmehr ◽  
Seyed Kamran Kamrava ◽  
Rafieh Alizadeh ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Research Work ◽  
Septal Cartilage ◽  
Cartilage Defects ◽  
Nasal Cartilage ◽  
Tissue Engineering Approach ◽  
Cell Sources ◽  
Engineering Strategy ◽  
New Perspective ◽  
Nasal Septal Cartilage

The nasal septal cartilage plays an important role in the growth of midface and as a vertical strut preventing the collapse of the nasal bones. The repair of nasal cartilage defects remains a major challenge in reconstructive surgery. The tissue engineering strategy in the development of tissue has opened a new perspective to generate functional tissue for transplantation. Given the poor regenerative properties of cartilage and a limited amount of autologous cartilage availability, intense interest has evoked for tissue engineering approaches for cartilage development to provide better outcomes for patients who require nasal septal reconstruction. Despite numerous attempts to substitute the shapely hyaline cartilage in the nasal cartilages, many significant challenges remained unanswered. The aim of this research was to carry out a critical review of the literature on research work carried out on the development of septal cartilage using a tissue engineering approach, concerning different cell sources, scaffolds and growth factors, as well as its clinical pathway and trials have already been carried out.

Long-term in vivo stability of rabbit nasal septal cartilage following laser cartilage reshaping: A pilot investigation

2005 ◽  
Vol 36 (2) ◽  
pp. 147-154 ◽  
Author(s):  
Amir M. Karamzadeh ◽  
John C. Chang ◽  
Sergio Diaz ◽  
Thomas E. Milner ◽  
Brian J.F. Wong
Keyword(s):  
Septal Cartilage ◽  
Pilot Investigation ◽  
Cartilage Reshaping ◽  
Nasal Septal Cartilage

Incorporation into a biodegradable hyaluronic acid matrix enhances in vivo efficacy of recombinant human interleukin 11 (rhIL11)

2006 ◽  
Vol 115 (2) ◽  
pp. 134-139 ◽  
Author(s):  
Akira Takagi ◽  
Noboru Yamashita ◽  
Tatsunobu Yoshioka ◽  
Yuuki Takaishi ◽  
Kiyo Nakanishi ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
In Vivo Efficacy ◽  
Interleukin 11
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