In vitro studies of Annona muricata L . extract‐loaded electrospun scaffolds for localized treatment of breast cancer

2021 ◽  
Author(s):  
Udom M. Akpan ◽  
Michael Pellegrini ◽  
Ali A. Salifu ◽  
John D. Obayemi ◽  
Theresa Ezenwafor ◽  
...  
Keyword(s):  
Breast Cancer ◽  
In Vitro Studies ◽  
Annona Muricata ◽  
Electrospun Scaffolds ◽  
Localized Treatment

Protective Effect of Isothiocyanates from Cruciferous Vegetables on Breast Cancer: Epidemiological and Preclinical Perspectives

2020 ◽  
Vol 20 ◽  
Author(s):  
Suong N.T. Ngo ◽  
Desmond B. Williams
Keyword(s):  
Breast Cancer ◽  
Protective Effect ◽  
Animal Studies ◽  
Cancer Survival ◽  
Breast Cancer Survival ◽  
Human Studies ◽  
In Vitro Studies ◽  
Cochrane Library ◽  
Cruciferous Vegetables

Background: The effect of cruciferous vegetable intake on breast cancer survival is controversial at present. Glucosinolates are the naturally occurring constituents found across the cruciferous vegetables. Isothiocyanates are produced from the hydrolysis of glucosinolates and this reaction is catalysed by the plant-derived enzyme myrosinase. The main isothiocyanates (ITCs) from cruciferous vegetables are sulforaphane, benzyl ITC, and phenethyl ITC, which had been intensively investigated over the last decade for their antibreast cancer effects. Objective: The aim of this article is to systematically review the evidence from all types of studies, which examined the protective effect of cruciferous vegetables and/or their isothiocyanate constituents on breast cancer. Methods: A systematic review was conducted in Pubmed, EMBASE, and the Cochrane Library from inception to 27 April 2020. Peerreviewed studies of all types (in vitro studies, animal studies, and human studies) were selected. Results: The systematic literature search identified 16 human studies, 4 animal studies, and 65 in vitro studies. The effect of cruciferous vegetables and/or their ITCs intake on breast cancer survival was found to be controversial and varied greatly across human studies. Most of these trials were observational studies conducted in specific regions, mainly in the US and China. Substantial evidence from in vitro and animal studies was obtained, which strongly supported the protective effect of sulforaphane and other ITCs against breast cancer. Evidence from in vitro studies showed sulforaphane and other ITCs reduced cancer cell viability and proliferation via multiple mechanisms and pathways. Isothiocyanates inhibited cell cycle, angiogenesis and epithelial mesenchymal transition, as well as induced apoptosis and altered the expression of phase II carcinogen detoxifying enzymes. These are the essential pathways which promote the growth and metastasis of breast cancer. Noticeably, benzyl ITC showed a significant inhibitory effect on breast cancer stem cells, a new dimension of chemoresistance in breast cancer treatment. Sulforaphane and other ITCs displayed anti-breast cancer effects at variable range of concentrations and benzyl isothiocyanate appeared to have a relatively smallest inhibitory concentration IC50. The mechanisms underlying the cancer protective effect of sulforaphane and other ITCs have also been highlighted in this article. Conclusion: Current preclinical evidence strongly supports the role of sulforaphane and other ITCs as potential therapeutic agents for breast cancer, either as adjunct therapy or combined therapy with current anti-breast cancer drugs, with sulforaphane appeared to display the greatest potential.

Assay of Gcdfp-15 by Elisa: An Available Method for in Vitro Studies of Functional Differentiation in Human Breast Cancer

1988 ◽  
Vol 74 (6) ◽  
pp. 669-674
Author(s):  
Françoise Revillion-Carette ◽  
Louis Hornez ◽  
Brigitte Vandewalle ◽  
Jean Lefebvre
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Culture Media ◽  
Functional Differentiation ◽  
In Vitro Studies ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Breast ◽  
Breast Cyst Fluid ◽  
Breast Cyst

An enzyme-linked immunosorbent assay (ELISA) was applied to a light protein, isolated from human breast cyst fluid (BCF) termed « gross cystic disease fluid protein - 15 Kda » (GCDFP-15), a potential differentiation marker in in vitro human breast cancer studies. The detection limits of this procedure, performed in microtiter plates, were 0.5 to 250 ng/well corresponding to 10 ng/ml to 5 ng/ml of sample or antigen solution. Possible cross-reaction with various antigens, especially those found in culture media, were investigated. The correlation coefficient between enzymoassay and radioimmunoassay was 0.978. The results showed that quantification of GCDFP-15 by ELISA is a specific and highly sensitive method. This procedure may be of interest in in vitro studies on the functional differentiation of breast cancer cells.

Characterization and carboplatin loaded chitosan nanoparticles for the chemotherapy against breast cancer in vitro studies

2017 ◽  
Vol 97 ◽  
pp. 115-122 ◽  
Author(s):  
Md. Asad Khan ◽  
Md. Zafaryab ◽  
Syed Hassan Mehdi ◽  
Javed Quadri ◽  
M. Moshahid A. Rizvi
Keyword(s):  
Breast Cancer ◽  
Chitosan Nanoparticles ◽  
In Vitro Studies

scholarly journals Synthesis, Spectral Characterization, in Vitro and in Silico Studies of Benzodioxin Pyrazoline derivatives

2020 ◽  
Vol 11 (2) ◽  
pp. 9126-9138
Keyword(s):  
Breast Cancer ◽  
In Silico ◽  
Oral Absorption ◽  
Docking Studies ◽  
In Vitro Studies ◽  
Bacterial Protein ◽  
Online Tools ◽  
In Silico Studies ◽  
Theoretical Results

The present study deals with the in silico and in vitro studies of DBDP derivatives, which is formed from the Michal-addition reaction of DihydroBenzo[b]Dioxin Chalcone Derivatives(DBDD) with hydrazine hydrate and carboxyethane. The DBDD were synthesized via Claisen condensation between substituted aldehyde and 1,4-(benzodioxan-6-yl)-methyl ketone. The newly arrived compounds were characterized by IR and NMR spectra. The structurally confirmed synthesized compounds were screened against 1UAG microbial protein, 1OQA cancer protein using auto dock software, and ADME properties also found by using (in silico) Swissadme and Molinspiration online tools. All the newly arrived DBDP compounds have passed the acceptable values of ADME (drug-likeness), medicinal property, and lead likeness in ADME prediction. Compound DBDP-9 scored better values in drug-likeness. It obeys the five basic rules (Lipinski, Ghose, Verber, Egan, and Muegge) of medicinal chemistry property, passed the PAINS, Brenk filters with 0 violation, and also have better lead likeness value. All the other compounds in this series also passed the above-mentioned properties with 1 or 2 violations only present in PAINS and Brenk filter. This theoretical results incitement to performed docking and in vitro studies of the DBDP derivatives. Docking studies results that the good I.S averse to 1 UAG bacterial protein than standard drugs and also give impact values in the docking against 1OQA breast cancer protein. Overall observation from the above studies, DBDP-9 has a maximum oral absorption value 91.36% with 0 violation alert in drug-likeness, medicinal property, and pharmacokinetics filter. DBDP-4 has a good I.S (-8.8), DBDP-2 has 4 numbers of HBI as standard, and all the DBDP 1-9 compounds have higher I.S than the standard and also have impact I.S against 1OQA breast cancer protein.

Propofol Effects in Breast Cancer Cell Progression: Evidences from In Vitro Studies

2019 ◽  
pp. 147-157
Author(s):  
Sabrina Bimonte ◽  
Marco Cascella ◽  
Aldo Giudice ◽  
Francesca Bifulco ◽  
Stefan Wirz ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
In Vitro Studies ◽  
Cell Progression

scholarly journals A systematic comparison of lipopolymers for siRNA delivery to multiple breast cancer cell lines: In vitro studies

2020 ◽  
Vol 102 ◽  
pp. 351-366
Author(s):  
Hamidreza Montazeri Aliabadi ◽  
Remant Bahadur K.C. ◽  
Emira Bousoik ◽  
Ryley Hall ◽  
Ashley Barbarino ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Sirna Delivery ◽  
Cancer Cell Lines ◽  
In Vitro Studies ◽  
Breast Cancer Cell Lines ◽  
Systematic Comparison

Activation of the Kinin-Forming System during Therapy with Cyclophosphamide

1974 ◽  
Vol 20 (1) ◽  
pp. 19-21 ◽  
Author(s):  
A Cuschieri
Keyword(s):  
Breast Cancer ◽  
Side Effects ◽  
Venous Blood ◽  
In Vitro Studies ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Peripheral Venous Blood ◽  
Human Whole Blood ◽  
Maximum Decrease

Abstract Venous blood kininogen is significantly decreased during therapy with cyclophosphamide in cases of advanced breast cancer. The maximum decrease in plasma kininogen coincided with onset of maximal leukopenia in the peripheral venous blood. In vitro studies showed that both human whole blood and mononuclear cell suspensions liberate free kinin when incubated with cyclophosphamide, but plateletfree human plasma does not. Evidently, the kinin-forming system is activated during therapy with cyclophosphamide, and this may account for some of its reported side effects. The in vitro studies suggest that this activation results from damage to peripheral blood leukocytes by the cyclophosphamide, with the release of intracellular proteases.

Synthesis and in vitro studies of biodegradable thiolated chitosan hydrogels for breast cancer therapy

2011 ◽  
Vol 48 (5) ◽  
pp. 747-752 ◽  
Author(s):  
Mohammad Reza Saboktakin ◽  
Roya M. Tabatabaie ◽  
Abel Maharramov ◽  
Mohammad Ali Ramazanov
Keyword(s):  
Breast Cancer ◽  
Cancer Therapy ◽  
In Vitro Studies ◽  
Breast Cancer Therapy ◽  
Thiolated Chitosan ◽  
Chitosan Hydrogels
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