Physically‐cross‐linked poly(vinyl alcohol) cell culture plate coatings facilitate preservation of cell–cell interactions, spheroid formation, and stemness

2021 ◽  
Author(s):  
Kathleen Molyneaux ◽  
Maria D. Wnek ◽  
Sonya E.L. Craig ◽  
Jason Vincent ◽  
Imani Rucker ◽  
...  
Keyword(s):  
Cell Culture ◽  
Vinyl Alcohol ◽  
Cell Interactions ◽  
Poly Vinyl Alcohol ◽  
Spheroid Formation ◽  
Culture Plate ◽  
Cell Cell

Influence of selective laser sintering process parameters on microstructure and physicochemical properties of poly(vinyl alcohol) for the production of scaffolds

2020 ◽  
Vol 26 (6) ◽  
pp. 1155-1164
Author(s):  
Camila Fernandes Higa ◽  
Thatyanne Gradowski ◽  
Selene Elifio-Esposito ◽  
Marcelo Fernandes de Oliveira ◽  
Paulo Inforçatti ◽  
...  
Keyword(s):  
Cell Culture ◽  
Process Parameters ◽  
Vinyl Alcohol ◽  
Laser Power ◽  
Selective Laser Sintering ◽  
Degree Of Crystallinity ◽  
Poly Vinyl Alcohol ◽  
Content Type ◽  
The Degree Of Crystallinity

Purpose This study aims to investigate the production of scaffolds by selective laser sintering (SLS) using poly(vinyl alcohol) (PVA) polymer, for in vitro studies, a relatively new and growing area in which scaffolds could be used in the design of three-dimensional models for in vitro disease model or tissue equivalent for safety and effectiveness tests. Design/methodology/approach The influence of the SLS process parameters laser power, 26 W and 32 W, and number of laser scans, 1, 2, 4 and 6, on the surface microstructure of the samples and on the degree of crystallinity and chemical stability of PVA material, was investigated using powder with particle size of 20-320 µm. Laser sintered PVA samples were subjected to cell culture tests using osteoblastic cells derived from human osteosarcoma (SaOs-2). Findings The laser power has no significant influence on the microstructure of the laser-sintered samples, however the number of scans has a considerable influence on the sintering degree; the SLS process causes a decrease in the degree of crystallinity and changes the chemical structure of the as-received PVA, especially when using higher laser power and more number of scans. Preliminary in vitro cell culture tests show that the laser-sintered PVA material is biocompatible with SaOs-2 cells. Originality/value SLS offers good potential for the fabrication of scaffolds and thus, may be applied as an alternative to conventional scaffold fabrication processes to overcome their limitations.

Poly(vinyl alcohol co-vinyl acetate) as a novel scaffold for mammalian cell culture and controlled drug release

2019 ◽  
Vol 54 (10) ◽  
pp. 7867-7882
Author(s):  
Francisca Villanueva-Flores ◽  
Margarita Miranda-Hernández ◽  
José O. Flores-Flores ◽  
Alberto Porras-Sanjuanico ◽  
Hailin Hu ◽  
...  
Keyword(s):  
Cell Culture ◽  
Drug Release ◽  
Vinyl Acetate ◽  
Mammalian Cell ◽  
Vinyl Alcohol ◽  
Mammalian Cell Culture ◽  
Controlled Drug Release ◽  
Poly Vinyl Alcohol ◽  
Novel Scaffold

Poly(vinyl alcohol-co-itaconic acid) hydrogels grafted with several designed peptides for human pluripotent stem cell culture and differentiation into cardiomyocytes

2021 ◽  
Author(s):  
Tzu-Cheng Sung ◽  
Ming-Wei Lu ◽  
Zeyu Tian ◽  
Henry Hsin-Chung Lee ◽  
Jiandong Pan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Itaconic Acid ◽  
Vinyl Alcohol ◽  
Pluripotent Stem Cell ◽  
Poly Vinyl Alcohol ◽  
Human Pluripotent Stem Cell ◽  
Stem Cell Culture

We developed poly(vinyl alcohol-co-itaconic acid) (PV) hydrogels grafted with laminin-derived peptides that had different joint segments and several specific designs, including dual chain motifs. PV hydrogels grafted with a peptide...

Investigation of the Competition Between Cell/Surface and Cell/Cell Interactions During Neuronal Cell Culture on a Micro-Engineered Surface

2013 ◽  
Vol 13 (11) ◽  
pp. 1546-1555 ◽  
Author(s):  
Amélie Béduer ◽  
Inès Gonzales-Calvo ◽  
Christophe Vieu ◽  
Isabelle Loubinoux ◽  
Laurence Vaysse
Keyword(s):  
Cell Culture ◽  
Cell Surface ◽  
Neuronal Cell ◽  
Cell Interactions ◽  
Neuronal Cell Culture ◽  
Cell Cell
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