scholarly journals Comparison of the formation, adipogenic maturation, and retention of human adipose‐derived stem cell spheroids in scaffold‐free culture techniques

2020 ◽  
Vol 108 (7) ◽  
pp. 3022-3032 ◽  
Author(s):  
Sarah J. Fitzgerald ◽  
Jared S. Cobb ◽  
Amol V. Janorkar
Keyword(s):  
Stem Cell ◽  
Culture Techniques ◽  
Cell Spheroids ◽  
Adipose Derived Stem Cell

Enhancement of human adipose-derived stem cell spheroid differentiation in an in situ enzyme-crosslinked gelatin hydrogel

2019 ◽  
Vol 7 (7) ◽  
pp. 1064-1075 ◽  
Author(s):  
Ching-Cheng Tsai ◽  
Yu-Jun Hong ◽  
Randall J. Lee ◽  
Nai-Chen Cheng ◽  
Jiashing Yu
Keyword(s):  
Stem Cell ◽  
Cell Spheroid ◽  
Cell Spheroids ◽  
Gelatin Hydrogel ◽  
Adipose Derived Stem Cell

Standardized human adipose-derived stem cell spheroids can be harvested abundantly and the differentiation capability of cell spheroids performed well in the enzyme-crosslinked gelatin hydrogel.

Hydrogels with an embossed surface: An all-in-one platform for mass production and culture of human adipose-derived stem cell spheroids

Biomaterials ◽  
2019 ◽  
Vol 188 ◽  
pp. 198-212 ◽  
Author(s):  
Se-jeong Kim ◽  
Jaesung Park ◽  
Hayeon Byun ◽  
Young-Woo Park ◽  
Luke G. Major ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mass Production ◽  
Cell Spheroids ◽  
Adipose Derived Stem Cell

Baculovirus-transduced, VEGF-expressing adipose-derived stem cell sheet for the treatment of myocardium infarction

Biomaterials ◽  
2014 ◽  
Vol 35 (1) ◽  
pp. 174-184 ◽  
Author(s):  
Tsung-Szu Yeh ◽  
Yu-Hua Dean Fang ◽  
Chia-Hsin Lu ◽  
Shao-Chieh Chiu ◽  
Chia-Lin Yeh ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Sheet ◽  
Myocardium Infarction ◽  
Adipose Derived Stem Cell

scholarly journals Core–shell hydrogel microcapsules enable formation of human pluripotent stem cell spheroids and their cultivation in a stirred bioreactor

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Pouria Fattahi ◽  
Ali Rahimian ◽  
Michael Q. Slama ◽  
Kihak Gwon ◽  
Alan M. Gonzalez-Suarez ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Scale Up ◽  
Bioreactor Culture ◽  
Stirred Bioreactor ◽  
Cell Spheroids ◽  
Poly Ethylene ◽  
Aqueous Core ◽  
End Stage ◽  
Stirred Bioreactors

AbstractCellular therapies based on human pluripotent stem cells (hPSCs) offer considerable promise for treating numerous diseases including diabetes and end stage liver failure. Stem cell spheroids may be cultured in stirred bioreactors to scale up cell production to cell numbers relevant for use in humans. Despite significant progress in bioreactor culture of stem cells, areas for improvement remain. In this study, we demonstrate that microfluidic encapsulation of hPSCs and formation of spheroids. A co-axial droplet microfluidic device was used to fabricate 400 μm diameter capsules with a poly(ethylene glycol) hydrogel shell and an aqueous core. Spheroid formation was demonstrated for three hPSC lines to highlight broad utility of this encapsulation technology. In-capsule differentiation of stem cell spheroids into pancreatic β-cells in suspension culture was also demonstrated.

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