scholarly journals Dual growth factor delivery using PLGA nanoparticles in silk fibroin/PEGDMA hydrogels for articular cartilage tissue engineering

2019 ◽  
Vol 108 (5) ◽  
pp. 2041-2062 ◽  
Author(s):  
Milad Fathi‐Achachelouei ◽  
Dilek Keskin ◽  
Erhan Bat ◽  
Nihal E. Vrana ◽  
Aysen Tezcaner
Keyword(s):  
Tissue Engineering ◽  
Articular Cartilage ◽  
Growth Factor ◽  
Silk Fibroin ◽  
Cartilage Tissue Engineering ◽  
Plga Nanoparticles ◽  
Cartilage Tissue ◽  
Growth Factor Delivery ◽  
Dual Growth Factor Delivery

scholarly journals Effect of initial cell seeding density on 3D-engineered silk fibroin scaffolds for articular cartilage tissue engineering

Biomaterials ◽  
2011 ◽  
Vol 32 (34) ◽  
pp. 8927-8937 ◽  
Author(s):  
Sarmistha Talukdar ◽  
Quynhhoa T. Nguyen ◽  
Albert C. Chen ◽  
Robert L. Sah ◽  
Subhas C. Kundu
Keyword(s):  
Tissue Engineering ◽  
Articular Cartilage ◽  
Silk Fibroin ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Seeding Density ◽  
Cell Seeding ◽  
Initial Cell ◽  
Cell Seeding Density

scholarly journals Fabrication and In Vitro Study of Tissue-Engineered Cartilage Scaffold Derived from Wharton’s Jelly Extracellular Matrix

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Tongguang Xiao ◽  
Weimin Guo ◽  
Mingxue Chen ◽  
Chunxiang Hao ◽  
Shuang Gao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Articular Cartilage ◽  
Growth Factor ◽  
Cartilage Tissue Engineering ◽  
Wharton’S Jelly ◽  
Immunofluorescent Staining ◽  
Cartilage Tissue ◽  
Wharton's Jelly ◽  
Engineered Cartilage

The scaffold is a key element in cartilage tissue engineering. The components of Wharton’s jelly are similar to those of articular cartilage and it also contains some chondrogenic growth factors, such as insulin-like growth factor I and transforming growth factor-β. We fabricated a tissue-engineered cartilage scaffold derived from Wharton’s jelly extracellular matrix (WJECM) and compared it with a scaffold derived from articular cartilage ECM (ACECM) using freeze-drying. The results demonstrated that both WJECM and ACECM scaffolds possessed favorable pore sizes and porosities; moreover, they showed good water uptake ratios and compressive moduli. Histological staining confirmed that the WJECM and ACECM scaffolds contained similar ECM. Moreover, both scaffolds showed good cellular adherence, bioactivity, and biocompatibility. MTT and DNA content assessments confirmed that the ACECM scaffold tended to be more beneficial for improving cell proliferation than the WJECM scaffold. However, RT-qPCR results demonstrated that the WJECM scaffold was more favorable to enhance cellular chondrogenesis than the ACECM scaffold, showing more collagen II and aggrecan mRNA expression. These results were confirmed indirectly by glycosaminoglycan and collagen content assessments and partially confirmed by histology and immunofluorescent staining. In conclusion, these results suggest that a WJECM scaffold may be favorable for future cartilage tissue engineering.

Biomarker Identification Under Growth Factor Priming for Cartilage Tissue Engineering

2012 ◽  
Author(s):  
Elena Alegre-Aguarón ◽  
Sonal R. Sampat ◽  
Perry J. Hampilos ◽  
J. Chloë Bulinski ◽  
James L. Cook ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Articular Cartilage ◽  
Growth Factor ◽  
Cartilage Repair ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Physical Factors ◽  
Functional Tissue ◽  
Cell Sources ◽  
The Impact

Adult articular cartilage has a poor healing capacity, which has lead to intense research toward development of cell-based therapies for cartilage repair. The destruction of articular cartilage results in osteoarthritis (OA), which affects about 27 million Americans. In order to create functional tissue, it is essential to mimic the native environment by optimizing expansion protocols. Cell passaging and priming with chemical or physical factors are often necessary steps in cell-based strategies for regenerative medicine [1]. The ability to identify biomarkers that can act as predictors of cells with a high capacity to form functional engineered cartilage will permit optimization of protocols for cartilage tissue engineering using different cell sources. Recent investigations have shown that chondrocytes and synovium-derived stem cells (SDSCs) are promising cell sources for cartilage repair [2,3]. The analysis of gene expression and comparative proteomics, which defines the differences in expression of proteins among different biological states, provides a potentially powerful tool in this effort [4]. The aim of this study was to investigate the impact of growth factor priming in 2D canine chondrocytes and SDSCs cultures by identifying differentially regulated biomarkers, which can correlate to functional tissue elaboration in 3D.

scholarly journals Potential of 3-D tissue constructs engineered from bovine chondrocytes/silk fibroin-chitosan for in vitro cartilage tissue engineering

Biomaterials ◽  
2011 ◽  
Vol 32 (25) ◽  
pp. 5773-5781 ◽  
Author(s):  
Nandana Bhardwaj ◽  
Quynhhoa T. Nguyen ◽  
Albert C. Chen ◽  
David L. Kaplan ◽  
Robert L. Sah ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Silk Fibroin ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Tissue Constructs

scholarly journals Hydrogels for the Application of Articular Cartilage Tissue Engineering: A Review of Hydrogels

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Er-Yuan Chuang ◽  
Chih-Wei Chiang ◽  
Pei-Chun Wong ◽  
Chih-Hwa Chen
Keyword(s):  
Tissue Engineering ◽  
Articular Cartilage ◽  
Cartilage Damage ◽  
Medical Science ◽  
Cartilage Tissue Engineering ◽  
Polymeric Materials ◽  
Tissue Growth ◽  
Cartilage Tissue ◽  
Cellular Interaction ◽  
Polymeric Hydrogels

The treatment of articular cartilage damage is a major task in the medical science of orthopedics. Hydrogels possess the ability to form multifunctional cartilage grafts since they possess polymeric swellability upon immersion in an aqueous phase. Polymeric hydrogels are capable of physiological swelling and greasing, and they possess the mechanical behavior required for use as articular cartilage substitutes. The chondrogenic phenotype of these materials may be enhanced by embedding living cells. Artificial hydrogels fabricated from biologically derived and synthesized polymeric materials are also used as tissue-engineering scaffolds; with their controlled degradation profiles, the release of stimulatory growth factors can be achieved. In order to make use of these hydrogels, cartilage implants were formulated in the laboratory to demonstrate the bionic mechanical behaviors of physiological cartilage. This paper discusses developments concerning the use of polymeric hydrogels for substituting injured cartilage tissue and assisting tissue growth. These gels are designed with consideration of their polymeric classification, mechanical strength, manner of biodegradation, limitations of the payload, cellular interaction, amount of cells in the 3D hydrogel, sustained release for the model drug, and the different approaches for incorporation into adjacent organs. This article also summarizes the different advantages, disadvantages, and the future prospects of hydrogels.

Sign in / Sign up

Export Citation Format

Share Document