scholarly journals Polymethyl methacrylate does not adversely affect the osteogenic potential of human adipose stem cells or primary osteoblasts

2020 ◽  
Vol 108 (4) ◽  
pp. 1536-1545
Author(s):  
Angela P. Bastidas‐Coral ◽  
Astrid D. Bakker ◽  
Cornelis J. Kleverlaan ◽  
Jolanda M. A. Hogervorst ◽  
Jenneke Klein‐Nulend ◽  
...  
Keyword(s):  
Stem Cells ◽  
Polymethyl Methacrylate ◽  
Adipose Stem Cells ◽  
Osteogenic Potential ◽  
Primary Osteoblasts ◽  
Human Adipose Stem Cells

scholarly journals Modulation of Human Adipose Stem Cells’ Neurotrophic Capacity Using a Variety of Growth Factors for Neural Tissue Engineering Applications: Axonal Growth, Transcriptional, and Phosphoproteomic Analyses In Vitro

Cells ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1939
Author(s):  
Katharina M. Prautsch ◽  
Alexander Schmidt ◽  
Viola Paradiso ◽  
Dirk J. Schaefer ◽  
Raphael Guzman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Neurotrophic Factor ◽  
Axonal Growth ◽  
Cellular Level ◽  
Adipose Stem Cells ◽  
Transcriptional Analysis ◽  
Axonal Outgrowth ◽  
Human Adipose Stem Cells

We report on a potential strategy involving the exogenous neurotrophic factors (NTF) for enhancing the neurotrophic capacity of human adipose stem cells (ASC) in vitro. For this, ASC were stimulated for three days using NTF, i.e., nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin 3 (NT3), NT4, glial cell-derived neurotrophic factor (GDNF), and ciliary neurotrophic factor (CNTF). The resulting conditioned medium (CM) as well as individual NTF exhibited distinct effects on axonal outgrowth from dorsal root ganglion (DRG) explants. In particular, CM derived from NT3-stimulated ASC (CM-NT3-ASC) promoted robust axonal outgrowth. Subsequent transcriptional analysis of DRG cultures in response to CM-NT3-ASC displayed significant upregulation of STAT-3 and GAP-43. In addition, phosphoproteomic analysis of NT3-stimulated ASC revealed significant changes in the phosphorylation state of different proteins that are involved in cytokine release, growth factors signaling, stem cell maintenance, and differentiation. Furthermore, DRG cultures treated with CM-NT3-ASC exhibited significant changes in the phosphorylation levels of proteins involved in tubulin and actin cytoskeletal pathways, which are crucial for axonal growth and elongation. Thus, the results obtained at the transcriptional, proteomic, and cellular level reveal significant changes in the neurotrophic capacity of ASC following NT3 stimulation and provide new options for improving the axonal growth-promoting potential of ASC in vitro.

scholarly journals Strategies for the hypothermic preservation of cell sheets of human adipose stem cells

PLoS ONE ◽  
2019 ◽  
Vol 14 (10) ◽  
pp. e0222597 ◽  
Author(s):  
Sara Freitas-Ribeiro ◽  
Andreia Filipa Carvalho ◽  
Marina Costa ◽  
Mariana Teixeira Cerqueira ◽  
Alexandra Pinto Marques ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Stem Cells ◽  
Cell Sheets ◽  
Hypothermic Preservation ◽  
Human Adipose Stem Cells

Exogenously added BMP-6, BMP-7 and VEGF may not enhance the osteogenic differentiation of human adipose stem cells

2013 ◽  
Vol 31 (5) ◽  
pp. 141-153 ◽  
Author(s):  
Laura Kyllönen ◽  
Suvi Haimi ◽  
Janne Säkkinen ◽  
Hannu Kuokkanen ◽  
Bettina Mannerström ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Adipose Stem Cells ◽  
Human Adipose Stem Cells

scholarly journals The effect of S53P4-based borosilicate glasses and glass dissolution products on the osteogenic commitment of human adipose stem cells

PLoS ONE ◽  
2018 ◽  
Vol 13 (8) ◽  
pp. e0202740 ◽  
Author(s):  
Miina Ojansivu ◽  
Ayush Mishra ◽  
Sari Vanhatupa ◽  
Miia Juntunen ◽  
Antonina Larionova ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Stem Cells ◽  
Borosilicate Glasses ◽  
Glass Dissolution ◽  
Human Adipose Stem Cells

Low serum and serum-free culture of multipotential human adipose stem cells

Cytotherapy ◽  
2007 ◽  
Vol 9 (7) ◽  
pp. 637-646 ◽  
Author(s):  
A.M. Parker ◽  
H. Shang ◽  
M. Khurgel ◽  
A.J. Katz
Keyword(s):  
Stem Cells ◽  
Adipose Stem Cells ◽  
Serum Free ◽  
Human Adipose Stem Cells ◽  
Low Serum

scholarly journals Recovery ability of human adipose stem cells exposed to cobalt nanoparticles: outcome of dissolution

Nanomedicine ◽  
2020 ◽  
Vol 15 (5) ◽  
pp. 453-465 ◽  
Author(s):  
Marina Borgese ◽  
Federica Rossi ◽  
Patrizia Bonfanti ◽  
Anita Colombo ◽  
Paride Mantecca ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Real Time Pcr ◽  
Slight Modification ◽  
Cobalt Nanoparticles ◽  
Normal Function ◽  
Adipose Stem Cells ◽  
Cell Recovery ◽  
Viability Test ◽  
Human Adipose Stem Cells

Aim: To demonstrate that cobalt nanoparticles doses are safe for use in humans and to understand the consequences of the particulate effects, which may persist inside the cells. Materials & methods: Human adipose stem cells were used. We evaluated cell recovery by viability test, morphology and ultrastructure using electronic and optical microscopy, while gene expression was assessed utilizing real-time PCR. Results: After exposure, most stem cells recovered their normal function. Co3O4-nanoparticles remained inside the cell for the entirety of the considered time. A slight modification of gene expression was observed in the exposed cells. Conclusion: After exposure to 100 M cobalt nanoparticles, most cells returned to normal function. Nanoparticle toxicity was due to ions released by dissolution as well as from the nanoparticles themselves.

Human adipose stem cells induced to osteogenic differentiation by an innovative collagen/hydroxylapatite hybrid scaffold

2017 ◽  
Vol 31 (10) ◽  
pp. 4555-4565 ◽  
Author(s):  
Elisa Mazzoni ◽  
Antonio D’Agostino ◽  
Marco Manfrini ◽  
Stefania Maniero ◽  
Andrea Puozzo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Adipose Stem Cells ◽  
Hybrid Scaffold ◽  
Human Adipose Stem Cells
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