A new approach to the production of a biovital skin graft based on human acellular dermal matrix produced in‐house, in vitro revitalized internally by human fibroblasts and keratinocytes on the surface

2020 ◽  
Vol 108 (4) ◽  
pp. 1281-1294
Author(s):  
Wojciech Łabuś ◽  
Diana Kitala ◽  
Agnieszka Klama‐Baryła ◽  
Michał Szapski ◽  
Wojciech Smętek ◽  
...  
Keyword(s):  
Skin Graft ◽  
Human Fibroblasts ◽  
Acellular Dermal Matrix ◽  
Dermal Matrix ◽  
New Approach ◽  
Acellular Dermal ◽  
Human Acellular Dermal Matrix

scholarly journals In Vitro Analysis of Histology, Mechanics, and Safety of Radiation-free Pre-hydrated Human Acellular Dermal Matrix

2020 ◽  
Vol 23 (6) ◽  
pp. 635
Author(s):  
Ji Young Kim ◽  
Kyung Min Yang ◽  
Ji Hyun Youn ◽  
Heejun Park ◽  
Hyung Min Hahn ◽  
...  
Keyword(s):  
Acellular Dermal Matrix ◽  
Dermal Matrix ◽  
Vitro Analysis ◽  
Acellular Dermal ◽  
Human Acellular Dermal Matrix ◽  
In Vitro Analysis

scholarly journals Acellular dermal matrix as filler in breast-conserving surgery: warnings for a careful use

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Gianluca Franceschini ◽  
Riccardo Masetti
Keyword(s):  
Acellular Dermal Matrix ◽  
Breast Conserving Surgery ◽  
Human Origin ◽  
Dermal Matrix ◽  
Adequate Knowledge ◽  
Aesthetic Results ◽  
Dermal Matrices ◽  
Acellular Dermal ◽  
Human Acellular Dermal Matrix ◽  
Host Tissues

AbstractAcellular dermal matrices are biological materials of porcine, bovine, or human origin used as scaffold for reconstructive purpose in plastic surgery; these materials are well-tolerated and safely integrated in host tissues without causing resorption, contracture, and encapsulation thanks to their low antigenicity.Recently, human acellular dermal matrix has been used as a filler in breast-conserving surgery to improve aesthetic results. Adequate knowledge of biomaterials properties, appropriate skill, and careful compliance with some specific recommendations are mandatory in order to optimize outcomes and obtain a work of success.

Construction of Tissue Engineered Skin with VEGF-Modified Human Mesenchymal Stem Cells and Acellular Dermal Matrix

2009 ◽  
Vol 610-613 ◽  
pp. 1298-1301
Author(s):  
Xiang Rong Zhang ◽  
De Wu Liu ◽  
Guang Hua Guo ◽  
Yan Peng
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Gradient Centrifugation ◽  
Human Mesenchymal Stem Cells ◽  
Acellular Dermal Matrix ◽  
Culture Method ◽  
Gene Encoding ◽  
Dermal Matrix ◽  
Acellular Dermal

The development of skin tissue engineering provides a noninvasive method for skin restoration. Unfortunately, the lack of a vascular plexus leads to greater time for vascularization compared with native skin autografts and contributes to graft failure. Our purpose was to construct tissue-engineered skin with VEGF- modified human bone marrow mesenchymal stem cells (hMSCs) as well as acellular dermal matrix(ADM) in vitro , Thus by increased vascular endothelial growth factor expression, which could prospectively improve vascularization of tissue-engineered skin for wound healing applications. To reach this aim, hMSCs were isolated and cultured with density gradient centrifugation combined with attachment culture method in vitro. Liposome- mediated gene transfer was used to generate a population of hMSCs overexpressing the gene encoding VEGF165. Then VEGF- modified hMSCs were seeded onto the surface of ADM. The experimental results showed that ADM we prepared has good compatibility with MSCs, the cells in ADM grew and proliferated well in vitro and the tissue - engineered skin with VEGF- modified hMSCs and ADM has been successfully constructed.

scholarly journals Reconstruction for Defects of Total Nail Bed and Germinal Matrix Loss with Acellular Dermal Matrix Coverage and Subsequently Skin Graft

Medicina ◽  
2020 ◽  
Vol 56 (1) ◽  
pp. 17
Author(s):  
Tsung-Hsien Liu ◽  
Meng-Chien Hsieh ◽  
Ping-Ruey Chou ◽  
Shu-Hung Huang
Keyword(s):  
Skin Graft ◽  
Donor Site ◽  
Acellular Dermal Matrix ◽  
Dermal Matrix ◽  
Germinal Matrix ◽  
Nail Bed ◽  
Staged Operation ◽  
Matrix Loss ◽  
Bone Exposure ◽  
Acellular Dermal

Background and Objectives: Nail bed and germinal matrix loss due to wide excision for fingertip tumors or malignancy are occasionally encountered complications. These defects also result from severely comminuted fingertip crush injuries. Large-area dorsal finger or toenail bed defects, which usually present with phalangeal bone exposure, remain challenging regardless of the usage of different reconstruction strategies. This study aimed to evaluate the clinical outcome of a staged operation with an acellular dermal matrix coverage and subsequent skin graft as reconstruction for defects of total nail bed, germinal matrix loss, and bone exposure. Materials and Methods: From April 2018 to October 2019, four patients with total nail bed, germinal matrix, and bone exposure loss after surgery were enrolled in our series. A staged operation of the acellular dermal matrix coverage with subsequent skin graft was performed on these patients. Skin graft take rate, oncological prognosis, and cosmetic outcome were evaluated. Patients were followed up for 5–13 months. An excellent skin graft take rate with a satisfying aesthetic result without local malignancy recurrence was noted. Minimal functional deficit and donor site morbidity were reported. Results: A staged operation with acellular dermal matrix coverage and subsequent skin graft proves to serve as a feasible strategy for patients who experience total nail bed, germinal matrix loss, and bone exposure after surgery. Conclusions: This reconstruction method provides a reliable repair result, satisfying aesthetic outcomes, as well as having minimal functional deficits and donor site morbidity.

Sign in / Sign up

Export Citation Format

Share Document