Determining the optimal protocol for preparing an acellular scaffold of tissue engineered small-diameter blood vessels

2017 ◽  
Vol 106 (2) ◽  
pp. 619-631 ◽  
Author(s):  
Lei Pu ◽  
Jian Wu ◽  
Xingna Pan ◽  
Zongliu Hou ◽  
Jing Zhang ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Small Diameter ◽  
Optimal Protocol ◽  
Acellular Scaffold

Scaffolds in tissue engineering of blood vessels

2010 ◽  
Vol 88 (9) ◽  
pp. 855-873 ◽  
Author(s):  
Divya Pankajakshan ◽  
Devendra K. Agrawal
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Blood Vessels ◽  
Vascular Tissue ◽  
Small Diameter ◽  
Biological Scaffolds ◽  
Hemodynamic Forces ◽  
Biodegradable Scaffolds

Tissue engineering of small diameter (<5 mm) blood vessels is a promising approach for developing viable alternatives to autologous vascular grafts. It involves in vitro seeding of cells onto a scaffold on which the cells attach, proliferate, and differentiate while secreting the components of extracellular matrix that are required for creating the tissue. The scaffold should provide the initial requisite mechanical strength to withstand in vivo hemodynamic forces until vascular smooth muscle cells and fibroblasts reinforce the extracellular matrix of the vessel wall. Hence, the choice of scaffold is crucial for providing guidance cues to the cells to behave in the required manner to produce tissues and organs of the desired shape and size. Several types of scaffolds have been used for the reconstruction of blood vessels. They can be broadly classified as biological scaffolds, decellularized matrices, and polymeric biodegradable scaffolds. This review focuses on the different types of scaffolds that have been designed, developed, and tested for tissue engineering of blood vessels, including use of stem cells in vascular tissue engineering.

3D Coculture of Human Endothelial Cells and Myofibroblasts for Vascular Tissue Engineering

2007 ◽  
Author(s):  
Rolf A. A. Pullens ◽  
Maria Stekelenburg ◽  
Carlijn V. C. Bouten ◽  
Frank P. T. Baaijens ◽  
Mark J. Post
Keyword(s):  
Tissue Engineering ◽  
Blood Vessels ◽  
Vascular Tissue ◽  
Artery Bypass ◽  
Small Diameter ◽  
Life Time ◽  
Mechanical Conditioning ◽  
Tissue Properties ◽  
Vein Grafts ◽  
Limited Life

Cardiovascular disease is still the number one cause of death in the industrialized world. Diseased small diameter blood vessels are frequently replaced by native grafts. However, these vessels have a limited life time [1], for example the patency at 10 year after coronary artery bypass grafting of saphenous vein grafts is 57% [2]. Tissue engineering (TE) of small diameter blood vessels seems a promising approach to overcome these shortcomings or address the increasing need for substitutes during follow up surgery. Mechanical conditioning of myofibroblast (MFs) seeded constructs appears to be beneficial for functional tissue properties, such as cell proliferation, ECM production and mechanical strength [3,4]. Without a functional endothelial cell (ECs) layer however, patency may be compromised by thrombogenecity. Construction of an EC layer might on the other hand affect the tissue composition during culture, as was shown for bovine ECs, which influenced proliferation and ECM production of smooth muscle cells [5].

Biomechanical Properties of Self-Assembly Tissue Engineered Blood Vessels: Insights Into Assembly Techniques

2010 ◽  
Author(s):  
Michael T. Zaucha ◽  
Rudolph Gleason
Keyword(s):  
Coronary Artery Disease ◽  
Blood Vessels ◽  
Self Assembly ◽  
Small Diameter ◽  
Biomechanical Properties ◽  
Industrialized Nations ◽  
Tissue Engineered Blood Vessels ◽  
Artery Disease ◽  
Thrombogenic Potential ◽  
Synthetic Grafts

Coronary artery disease remains to be the leading cause of morbidity and mortality in industrialized nations. Current treatments for small diameter grafts are limited by the availability of suitable autologous vessels and high thrombogenic potential of synthetic grafts. There is a clinical need to development of tissue engineered blood vessels (TEBV) suitable for vascular by pass grafting.

Development of Small-Diameter Vascular Grafts Through Decellularization of Human Blood Vessels

2017 ◽  
Vol 7 (2) ◽  
pp. 101-110 ◽  
Author(s):  
Andrea Porzionato ◽  
Maria Martina Sfriso ◽  
Alex Pontini ◽  
Veronica Macchi ◽  
Maria Ida Buompensiere ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Human Blood ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Small Diameter Vascular Grafts

scholarly journals Feasibility and Safety of Unzipping Small Diameter Stents in the Blood Vessels of Piglets

2016 ◽  
Vol 9 (11) ◽  
pp. 1138-1149 ◽  
Author(s):  
Shyam K. Sathanandam ◽  
T.K. Susheel Kumar ◽  
Deepthi Hoskoppal ◽  
Lauren M. Haddad ◽  
Saradha Subramanian ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Small Diameter

Fabrication and Preliminary Study of a Prototype Bi-Layered Small Diameter Vascular Prosthesis Composed of Nano-Fiber and Silk Fiber

2013 ◽  
Vol 843 ◽  
pp. 66-69 ◽  
Author(s):  
Hui Jing Zhao ◽  
Guo Li Zhou ◽  
Zhi Qing Yuan
Keyword(s):  
Mechanical Properties ◽  
Blood Vessels ◽  
Outer Layer ◽  
Small Diameter ◽  
Peak Stress ◽  
Silk Fiber ◽  
Content Increase ◽  
Peak Strain ◽  
Tissue Engineering Scaffolds ◽  
Vascular Prostheses

Biomaterials used for vascular prostheses should possess certain strength that can keep the normal blood fluidity, as well as certain flexibility and elasticity that can resist blood pulsation pressure. In order to fabricate small diameter vascular prostheses (SDVP) that possess matchable mechanical properties with natural blood vessels, a bi-layered tubular structure composed of electrospinning blended nanofiber and silk fiber was designed and prepared in this study. The inner layer of the structure, prepared through electrospinning, was composed of Poly (L-lactide-co-ε-caprolactone) (PLCL) and silk fibroin (SF) blended nanofibers. Braided silk tube was used as the outer layer of the structure. Morphological, structural and mechanical properties including peak stress, peak strain, and Youngs modulus of the prototype bi-layered SDVP were characterized initially. Results showed that the diameter range of the blended nanofiber was between 100 and 900 nm, and the fiber diameter increased with the content increase of PLCL. Through blending PLCL together with SF, peak stress and peak strain of the electrospun inner layer were improved, and that of the Youngs modulus decreased. Meanwhile, the outer layer of SDVP was stronger and had higher Youngs modulus. Those mechanical performances of the prototype bi-layered SDVP fabricated in this study are similar to natural blood vessels, which provide a promising biomaterial that could be applied on tubular tissue engineering scaffolds.

Electrospun polylactide/silk fibroin-gelatin composite tubular scaffolds for small-diameter tissue engineering blood vessels

2009 ◽  
Vol 113 (4) ◽  
pp. 2675-2682 ◽  
Author(s):  
Shudong Wang ◽  
Youzhu Zhang ◽  
Guibo Yin ◽  
Hongwei Wang ◽  
Zhihui Dong
Keyword(s):  
Tissue Engineering ◽  
Blood Vessels ◽  
Silk Fibroin ◽  
Small Diameter

Theory and Experiments for Mechanically-Induced Remodeling of Tissue Engineered Blood Vessels

2008 ◽  
Vol 57 ◽  
pp. 226-234 ◽  
Author(s):  
Rudolph L. Gleason ◽  
William Wan
Keyword(s):  
Mechanical Properties ◽  
Blood Vessels ◽  
Laser Scanning ◽  
Biomechanical Testing ◽  
Small Diameter ◽  
Mechanical Stimuli ◽  
Two Photon ◽  
Constrained Mixture ◽  
Tissue Engineered Blood Vessels ◽  
Luminal Flow

There is a great unmet clinical need to develop small diameter tissue engineered blood vessels (TEBV) with low thrombogenicity and immune response and suitable mechanical properties. In this paper we describe experimental and computational frameworks to characterize the use of mechanical stimuli to improve the mechanical properties of TEBVs. We model the TEBV as a constrained mixture and track the production, degradation, mechanical state, and organization of each structural constituent. Specifically, we assume that individual load bearing constituents can co-exist within each neighborhood and, although they are constrained to deform together, each constituent within this neighborhood may have different natural (i.e., stress-free) configurations. Motivated by this theoretical framework, we have designed a bioreactor and biomechanical testing device for TEBVs. This device is designed to provide precise and independent control of mean and cyclic luminal flow rate, transmural pressure, and axial load over weeks and months in culture and perform intermittent biaxial biomechanical tests. This device also fits under a two-photon laser scanning microscope for 3-dimenstional imaging of the content and organization of cells and matrix constituents. These data directly support our theoretical model.

scholarly journals Blood Vessel-Derived Acellular Matrix for Vascular Graft Application

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Luigi Dall’Olmo ◽  
Ilenia Zanusso ◽  
Rosa Di Liddo ◽  
Tatiana Chioato ◽  
Thomas Bertalot ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Immunomagnetic Separation ◽  
Enzymatic Digestion ◽  
Enzymatic Treatment ◽  
Acellular Matrix ◽  
Cell Coverage ◽  
Class Ii Antigens

To overcome the issues connected to the use of autologous vascular grafts and artificial materials for reconstruction of small diameter (<6 mm) blood vessels, this study aimed to develop acellular matrix- (AM-) based vascular grafts. Rat iliac arteries were decellularized by a detergent-enzymatic treatment, whereas endothelial cells (ECs) were obtained through enzymatic digestion of rat skin followed by immunomagnetic separation of CD31-positive cells. Sixteen female Lewis rats (8 weeks old) received only AM or previouslyin vitroreendothelialized AM as abdominal aorta interposition grafts (about 1 cm). The detergent-enzymatic treatment completely removed the cellular part of vessels and both MHC class I and class II antigens. One month after surgery, the luminal surface of implanted AMs was partially covered by ECs and several platelets adhered in the areas lacking cell coverage. Intimal hyperplasia, already detected after 1 month, increased at 3 months. On the contrary, all grafts composed by AM and ECs were completely covered at 1 month and their structure was similar to that of native vessels at 3 months. Taken together, our findings show that prostheses composed of AM preseeded with ECs could be a promising approach for the replacement of blood vessels.

Preparation and Characterization of Collagen-Elastin Matrices From Blood Vessels Intended as Small Diameter Vascular Grafts

2000 ◽  
Vol 24 (3) ◽  
pp. 217-223 ◽  
Author(s):  
Gilberto Goissis ◽  
Sueli Suzigan ◽  
Diderot Rodrigues Parreira ◽  
Jose Vitor Maniglia ◽  
Domingo Marcolino Braile ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Small Diameter Vascular Grafts
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