Ureteral double-J stents performances toward encrustation after long-term indwelling in a dynamic in vitro model

2016 ◽  
Vol 105 (8) ◽  
pp. 2244-2253 ◽  
Author(s):  
Valentina Cauda ◽  
Angelica Chiodoni ◽  
Marco Laurenti ◽  
Giancarlo Canavese ◽  
Tonia Tommasi
Keyword(s):  
In Vitro Model ◽  
Vitro Model

scholarly journals A Pilot Study To Establish an In Vitro Model To Study Premature Intestinal Epithelium and Gut Microbiota Interactions

mSphere ◽  
2021 ◽  
Author(s):  
Justin Gibbons ◽  
Ji Youn Yoo ◽  
Tina Mutka ◽  
Maureen Groer ◽  
Thao T. B. Ho
Keyword(s):  
Cell Culture ◽  
In Vitro Model ◽  
Bacterial Flora ◽  
Mucosal Barrier ◽  
Clinical Observations ◽  
Culture Models ◽  
Vitro Model ◽  
Cell Culture Models

The gut bacterial flora influences the development of the immune system and long-term health outcomes in preterm infants. Studies of the mechanistic interactions between the gut bacteria and mucosal barrier are limited to clinical observations, animal models, and in vitro cell culture models for this vulnerable population.

Electrospun nanofibers facilitate better alignment, differentiation, and long-term culture in an in vitro model of the neuromuscular junction (NMJ)

2018 ◽  
Vol 6 (12) ◽  
pp. 3262-3272 ◽  
Author(s):  
Baiwen Luo ◽  
Lingling Tian ◽  
Nuan Chen ◽  
Seeram Ramakrishna ◽  
Nitish Thakor ◽  
...  
Keyword(s):  
Neuromuscular Junction ◽  
Culture System ◽  
In Vitro Model ◽  
Electrospun Nanofibers ◽  
Nanofibrous Scaffold ◽  
Long Term Culture ◽  
Vitro Model

An electrospun nanofibrous scaffold is used as a novel in vitro culture system to provide long-term support for NMJ formation.

Schlafen 11 is a novel target for mucosal regeneration in Ulcerative Colitis

2021 ◽  
Author(s):  
Sho Watanabe ◽  
Ryu Nishimura ◽  
Tomoaki Shirasaki ◽  
Nobuhiro Katsukura ◽  
Shuji Hibiya ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Microarray Analysis ◽  
In Vitro Model ◽  
Mucosal Injury ◽  
Mucosal Regeneration ◽  
Vitro Model ◽  
Novel Target

Abstract Background and Aims Ulcerative colitis (UC) is a chronic inflammatory disease of the colon with an intractable course. Although the goal of UC therapy is to achieve mucosal healing, the pathogenesis of mucosal injury caused by chronic inflammation remains unknown. We therefore aim to elucidate molecular mechanisms of mucosal injury by establishing in vitro and in vivo humanized UC mimicking models. Methods An in vitro model using human colon organoids was established by 60 weeks of inflammatory stimulation. The key gene for mucosal injury caused by long-term inflammation was identified by microarray analysis. An in vivo model was established by xenotransplantation of organoids into mouse colonic mucosa. Results An in vitro model demonstrated that long-term inflammation induced irrecoverable changes in organoids: inflammatory response and apoptosis with oxidative stress and suppression of cell viability. This model also mimicked organoids derived from patients with UC at the gene expression and phenotype levels. Microarray analysis revealed Schlafen11 (SLFN11) was irreversibly induced by long-term inflammation. Consistently, SLFN11 was highly expressed in UC mucosa but absent in normal mucosa. The knockdown of SLFN11 (SLFN11-KD) suppressed apoptosis of IECs induced by inflammation. Moreover, SLFN11-KD improved the take rates of xenotransplantation and induced regenerative changes of crypts observed in patients with UC in remission. Conclusions In vitro and in vivo UC mimicking models were uniquely established using human colonic organoids. They revealed SLFN11 is significant for mucosal injury in UC, and its potential as a novel target for mucosal regeneration.

Utility of Long-Term Cultured Human Hepatocytes as an in Vitro Model for Cytochrome P450 Induction

2006 ◽  
Vol 35 (2) ◽  
pp. 215-220 ◽  
Author(s):  
Georgina Meneses-Lorente ◽  
Christine Pattison ◽  
Claire Guyomard ◽  
Christophe Chesné ◽  
Robert Heavens ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
In Vitro Model ◽  
Human Hepatocytes ◽  
Cytochrome P450 Induction ◽  
Vitro Model
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