Effects of processing parameters in thermally induced phase separation technique on porous architecture of scaffolds for bone tissue engineering

2014 ◽  
Vol 102 (6) ◽  
pp. 1304-1315 ◽  
Author(s):  
Rosa Akbarzadeh ◽  
Azizeh-Mitra Yousefi
Keyword(s):  
Tissue Engineering ◽  
Phase Separation ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Processing Parameters ◽  
Separation Technique ◽  
Thermally Induced Phase Separation ◽  
Thermally Induced ◽  
Porous Architecture

Preparation of PLLA/HAP/β-TCP Composite Scaffold for Bone Tissue Engineering

2014 ◽  
Vol 513-517 ◽  
pp. 143-146 ◽  
Author(s):  
Xue Jun Wang ◽  
Tao Lou ◽  
Jing Yang ◽  
Zhen Yang ◽  
Kun Peng He
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Lactic Acid ◽  
Phase Separation ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Composite Scaffold ◽  
Thermally Induced Phase Separation ◽  
Composite Scaffolds ◽  
Thermally Induced

In this study, a nanofibrous poly (L-lactic acid) (PLLA) scaffold reinforced by Hydroxyapatite (HAP) and β-tricalcium phosphate (β-TCP) was fabricated using the thermally induced phase separation method. The composite scaffold morphology showed a nanofibrous PLLA matrix and evenly distributed β-TCP/HAP particles. The composite scaffold had interconnective micropores and the pore size ranged 2-10 μm. Introducing β-TCP/HAP particles into PLLA matrix significantly improved the mechanical properties of the composite scaffold. In summary, the new composite scaffolds show a great deal promise for use in bone tissue engineering.

scholarly journals Fabrication techniques involved in developing the composite scaffolds PCL/HA nanoparticles for bone tissue engineering applications

2021 ◽  
Vol 32 (8) ◽  
Author(s):  
Sivasankar Murugan ◽  
Sreenivasa Rao Parcha
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Freeze Drying ◽  
Composite Scaffold ◽  
Sol Gel ◽  
Bone Tissue Regeneration ◽  
Thermally Induced Phase Separation ◽  
Engineering Applications ◽  
Thermally Induced

AbstractA fine-tuned combination of scaffolds, biomolecules, and mesenchymal stem cells (MSCs) is used in tissue engineering to restore the function of injured bone tissue and overcome the complications associated with its regeneration. For two decades, biomaterials have attracted much interest in mimicking the native extracellular matrix of bone tissue. To this aim, several approaches based on biomaterials combined with MSCs have been amply investigated. Recently, hydroxyapatite (HA) nanoparticles have been incorporated with polycaprolactone (PCL) matrix as a suitable substitute for bone tissue engineering applications. This review article aims at providing a brief overview on PCL/HA composite scaffold fabrication techniques such as sol–gel, rapid prototyping, electro-spinning, particulate leaching, thermally induced phase separation, and freeze-drying, as suitable approaches for tailoring morphological, mechanical, and biodegradability properties of the scaffolds for bone tissues. Among these methods, the 3D plotting method shows improvements in pore architecture (pore size of ≥600 µm and porosity of 92%), mechanical properties (higher than 18.38 MPa), biodegradability, and good bioactivity in bone tissue regeneration.

scholarly journals Water Uptake in PHBV/Wollastonite Scaffolds: A Kinetics Study

2019 ◽  
Vol 3 (3) ◽  
pp. 74 ◽  
Author(s):  
Ribas ◽  
Montanheiro ◽  
Montagna ◽  
Prado ◽  
Campos ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Phase Separation ◽  
Cell Growth ◽  
Cell Viability ◽  
Water Uptake ◽  
Kinetic Mechanism ◽  
Kinetics Study ◽  
Biological Applications ◽  
Thermally Induced Phase Separation ◽  
Thermally Induced

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) is a widely studied polymer and it has been found that porous PHBV materials are suitable for substrates for cell cultures. A crucial factor for scaffolds designed for tissue engineering is the water uptake. This property influences the transport of water and nutrients into the scaffold, which promotes cell growth. PHBV has significant hydrophobicity, which can harm the production of cells. Thus, the addition of α-wollastonite (WOL) can modify the PHBV scaffold’s water uptake. To our knowledge, a kinetics study of water uptake of α-wollastonite phase powder and the PHBV matrix has not been reported. In this work, PHBV and WOL, (PHBV/WOL) films were produced with 0, 5, 10, and 20 wt % of WOL. Films were characterized, and the best concentrations were chosen to produce PHBV/WOL scaffolds. The addition of WOL in concentrations up to 10 wt % increased the cell viability of the films. MTT analysis showed that PHBV/5%WOL and PHBV/10%WOL obtained cell viability of 80% and 98%, respectively. Therefore, scaffolds with 0, 5 and 10 wt % of WOL were fabricated by thermally induced phase separation (TIPS). Scaffolds were characterized with respect to morphology and water uptake in assay for 65 days. The scaffold with 10 wt % of WOL absorbed 44.1% more water than neat PHBV scaffold, and also presented a different kinetic mechanism when compared to other samples. Accordingly, PHBV/WOL scaffolds were shown to be potential candidates for biological applications.

Synthesis and characterization of nanofibrous hollow microspheres with tunable size and morphology via thermally induced phase separation technique

RSC Advances ◽  
2015 ◽  
Vol 5 (76) ◽  
pp. 61580-61585 ◽  
Author(s):  
Wei Feng ◽  
Zhiqi Yin ◽  
Weizhong Wang ◽  
Liang Chen ◽  
Xiaojun Zhou ◽  
...  
Keyword(s):  
Phase Separation ◽  
Hollow Microspheres ◽  
Separation Technique ◽  
Synthesis And Characterization ◽  
Thermally Induced Phase Separation ◽  
Thermally Induced ◽  
Size And Morphology

Nanofibrous hollow microspheres with tunable size and morphology were fabricated by using the thermally induced phase separation technique.

Bio-safe fabrication of PLA scaffolds for bone tissue engineering by combining phase separation, porogen leaching and scCO2 drying

2015 ◽  
Vol 97 ◽  
pp. 238-246 ◽  
Author(s):  
Aurelio Salerno ◽  
Mar Fernández-Gutiérrez ◽  
Julio San Román del Barrio ◽  
Concepción Domingo
Keyword(s):  
Tissue Engineering ◽  
Phase Separation ◽  
Bone Tissue Engineering ◽  
Bone Tissue

scholarly journals Biomimetic cellulose/calcium-deficient-hydroxyapatite composite scaffolds fabricated using an electric field for bone tissue engineering

RSC Advances ◽  
2018 ◽  
Vol 8 (37) ◽  
pp. 20637-20647 ◽  
Author(s):  
MyoJin Kim ◽  
MiJi Yeo ◽  
Minseong Kim ◽  
GeunHyung Kim
Keyword(s):  
Tissue Engineering ◽  
Electric Field ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Processing Parameters ◽  
Composite Scaffolds ◽  
Mesh Structure ◽  
Ceramic Scaffold ◽  
Hydroxyapatite Composite

The fabricated ceramic scaffold showed a layer-by-layered mesh structure entangled with cellulose micro/nanofibers and the bioceramic phase. By varying processing parameters, the unique 3D fibrous mesh-structure could be achieved.

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