In vitroresponse of osteoarthritic chondrocytes and fibroblast-like synoviocytes to a 500-730 kDa hyaluronan amide derivative

2012 ◽  
Vol 100B (8) ◽  
pp. 2073-2081 ◽  
Author(s):  
Paola Brun ◽  
Barbara Zavan ◽  
Vincenzo Vindigni ◽  
Antonella Schiavinato ◽  
Assunta Pozzuoli ◽  
...  
Keyword(s):  
Amide Derivative ◽  
Osteoarthritic Chondrocytes ◽  
Fibroblast Like Synoviocytes

scholarly journals The novel H2S-releasing compound D-P* reduces inflammation in osteoarthritic chondrocytes and fibroblast-like synoviocytes

2020 ◽  
Vol 28 ◽  
pp. S211-S212
Author(s):  
A. Fischer ◽  
N. Udovicic ◽  
R. Byrne ◽  
S. Bauer ◽  
J. Breitenbach ◽  
...  
Keyword(s):  
The Novel ◽  
Osteoarthritic Chondrocytes ◽  
Fibroblast Like Synoviocytes

scholarly journals Effect of growth hormone and hyaluronan amide derivative on human osteoarthritic chondrocytes

2015 ◽  
Vol 23 ◽  
pp. A163
Author(s):  
F. Paolella ◽  
E. Gabusi ◽  
C. Manferdini ◽  
L. Gambari ◽  
A. Schiavinato ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Amide Derivative ◽  
Osteoarthritic Chondrocytes

Age-independent effects of hyaluronan amide derivative and growth hormone on human osteoarthritic chondrocytes

2015 ◽  
Vol 56 (6) ◽  
pp. 440-451 ◽  
Author(s):  
Elena Gabusi ◽  
Francesca Paolella ◽  
Cristina Manferdini ◽  
Laura Gambari ◽  
Antonella Schiavinato ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Amide Derivative ◽  
Osteoarthritic Chondrocytes ◽  
Independent Effects ◽  
Age Independent

Treatment with Melittin Induces Apoptosis and Autophagy of Fibroblast-like Synoviocytes in Patients with Rheumatoid Arthritis

2020 ◽  
Vol 21 (8) ◽  
pp. 734-740 ◽  
Author(s):  
Shou-di He ◽  
Ning Tan ◽  
Chen-xia Sun ◽  
Kang-han Liao ◽  
Hui-jun Zhu ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Caspase 3 ◽  
Joint Destruction ◽  
Joint Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Caspase 9 ◽  
Inflammatory Processes ◽  
Related Proteins ◽  
Local Stimulation ◽  
Fibroblast Like Synoviocytes

Background: Melittin, the major medicinal component of honeybee venom, exerts antiinflammatory, analgesic, and anti-arthritic effects in patients with Rheumatoid Arthritis (RA). RA is an inflammatory autoimmune joint disease that leads to irreversible joint destruction and functional loss. Fibroblast-Like Synoviocytes (FLS) are dominant, special mesenchymal cells characterized by the structure of the synovial intima, playing a crucial role in both the initiation and progression of RA. Objective: In this study, we evaluated the effects of melittin on the viability and apoptosis of FLS isolated from patients with RA. Methods: Cell viability was determined using CCK-8 assays; apoptosis was evaluated by flow cytometry, and the expression levels of apoptosis-related proteins (caspase-3, caspase-9, BAX, and Bcl-2) were also determined. To explore whether melittin alters inflammatory processes in RA-FLS, IL-1β levels were determined using an enzyme-linked immunosorbent assay (ELISA). Furthermore, we performed GFP-LC3 punctate fluorescence dot assays and western blotting (for LC3, ATG5, p62, and Beclin 1) to assess autophagy in RA-FLS. Results: Our results show that melittin can significantly impair viability, promote apoptosis and autophagy, and inhibit IL-1β secretion in RA-FLS. Conclusion: Melittin may be useful in preventing damage to the joints during accidental local stimulation.

Mitochondrial Pathology in Osteoarthritic Chondrocytes

2014 ◽  
Vol 15 (7) ◽  
pp. 710-719 ◽  
Author(s):  
Longhuo Wu ◽  
Haiqing Liu ◽  
Linfu Li ◽  
Hai Liu ◽  
Qilai Cheng ◽  
...  
Keyword(s):  
Osteoarthritic Chondrocytes ◽  
Mitochondrial Pathology

THU0028 Microrna-381 Ameliorates Arthritis by Controlling Functions of Fibroblast-like Synoviocytes, Which Are Affected by A To I RNA Editing

2016 ◽  
Vol 75 (Suppl 2) ◽  
pp. 187.2-187
Author(s):  
Y. Tanaka ◽  
S. Takada ◽  
H. Iizasa ◽  
A. Hatzigeorgiou ◽  
S.-I. Miyazawa ◽  
...  
Keyword(s):  
Rna Editing ◽  
Fibroblast Like Synoviocytes
Sign in / Sign up

Export Citation Format

Share Document