Type I collagen gel in seeding medium improves murine mesencymal stem cell loading onto the scaffold, increases their subsequent proliferation, and enhances culture mineralization

2009 ◽  
Vol 90B (2) ◽  
pp. 659-667 ◽  
Author(s):  
Mohamadreza Baghaban Eslaminejad ◽  
Hamid Mirzadeh ◽  
Aghbibi Nickmahzar ◽  
Yossef Mohamadi ◽  
Houri Mivehchi
Keyword(s):  
Stem Cell ◽  
Type I Collagen ◽  
Collagen Gel ◽  
Type I ◽  
Cell Loading ◽  
Type I Collagen Gel

Apical beta 1 integrin in polarized MDCK cells mediates tubulocyst formation in response to type I collagen overlay

1996 ◽  
Vol 109 (7) ◽  
pp. 1875-1889 ◽  
Author(s):  
A. Zuk ◽  
K.S. Matlin
Keyword(s):  
Plasma Membranes ◽  
Type I Collagen ◽  
Mdck Cells ◽  
Basolateral Membrane ◽  
Collagen Gel ◽  
Type I ◽  
Membrane Markers ◽  
Polarized Epithelia ◽  
Beta 1 Integrin ◽  
Type I Collagen Gel

A number of epithelia form tubulocysts in vitro when overlaid with type I collagen gel. Because collagen receptors are generally believed to be expressed on the basolateral domain, the mechanism by which collagen elicits this morphogenetic response from the apical surface is unclear. To investigate the role of beta 1 integrins, the major receptor family for collagen, in this process, we overlaid polarized monolayers of MDCK II cells grown on permeable supports with type I collagen gel and correlated integrin polarity with the polarity of other apical and basolateral membrane markers during tubulocyst formation. Polarized monolayers of one clone of MDCK II cells, referred to as Heidelberg MDCK, initially respond to collagen overlay by stratifying; within 48 hours, lumena develop between the cell layers giving rise to tubulocysts. Tight junctions remain intact during tubulocyst formation because transepithelial electrical resistance does not significantly change. Major alterations are observed, however, in the expression and localization of apical and basolateral membrane markers. beta 1 integrins are necessary for tubulocyst morphogenesis because a function-blocking antibody administered to the apical pole of the cells completely inhibits the formation of these structures. To determine how apical-cell collagen interactions elicit tubulocyst formation, we examined whether beta 1 integrins are mobilized to apical plasma membranes in response to collagen overlay. We found that in the absence of collagen, polarized monolayers of Heidelberg MDCK cells endogenously express on apical plasma membranes a small pool of the beta 1 family, including alpha 2 beta 1 and alpha 3 beta 1. Collagen overlay does not mobilize additional beta 1 integrins to apical domains. If beta 1 integrins are not already apically expressed, as in the C6 MDCK cell line (Schoenenberger et al. (1994) J. Cell Biol. 107, 527–541), beta 1 integrins are not directed apically and tubulocysts do not develop in response to collagen. Thus, interaction of beta 1 integrin pre-existing on apical plasma membranes of polarized epithelia with type I collagen gel is the mechanism by which apical application of collagen elicits the formation of tubulocysts. Depolarized integrins on apical plasma membranes of polarized epithelia may be relevant to the pathogenesis of disease and injury.

The Physicochemical Property of Shark Type I Collagen Gel and Membrane

2000 ◽  
Vol 48 (6) ◽  
pp. 2028-2032 ◽  
Author(s):  
Yoshihiro Nomura ◽  
Shinzi Toki ◽  
Yasuhiro Ishii ◽  
Kunio Shirai
Keyword(s):  
Physicochemical Property ◽  
Type I Collagen ◽  
Collagen Gel ◽  
Type I ◽  
Type I Collagen Gel

Effects of tensile and compressive strains on response of a chondrocytic cell line embedded in type I collagen gel

2008 ◽  
Vol 133 (2) ◽  
pp. 245-252 ◽  
Author(s):  
Yuji Hirano ◽  
Naoki Ishiguro ◽  
Masahiro Sokabe ◽  
Masaharu Takigawa ◽  
Keiji Naruse
Keyword(s):  
Cell Line ◽  
Type I Collagen ◽  
Collagen Gel ◽  
Type I ◽  
Type I Collagen Gel

Bovine chondrocyte behaviour in three-dimensional type I collagen gel in terms of gel contraction, proliferation and gene expression

Biomaterials ◽  
2006 ◽  
Vol 27 (1) ◽  
pp. 79-90 ◽  
Author(s):  
Laurent Galois ◽  
Sandrine Hutasse ◽  
Delphine Cortial ◽  
Cécile F. Rousseau ◽  
Laurent Grossin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Type I Collagen ◽  
Three Dimensional ◽  
Collagen Gel ◽  
Type I ◽  
Type I Collagen Gel

scholarly journals Differentiation of embryonic stem cells into fibroblast-like cells in three-dimensional type I collagen gel cultures

2010 ◽  
Vol 47 (2) ◽  
pp. 114-124 ◽  
Author(s):  
Shinsaku Togo ◽  
Tadashi Sato ◽  
Hisatoshi Sugiura ◽  
Xingqi Wang ◽  
Hesham Basma ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Type I Collagen ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Collagen Gel ◽  
Type I ◽  
Type I Collagen Gel
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