The role of biological extracellular matrix scaffolds in vascularized three-dimensional tissue growthin vivo

2007 ◽  
Vol 82B (1) ◽  
pp. 122-128 ◽  
Author(s):  
Kevin J. Cronin ◽  
Aurora Messina ◽  
Erik W. Thompson ◽  
Wayne A. Morrison ◽  
Geoffrey W. Stevens ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Three Dimensional ◽  
Extracellular Matrix Scaffolds

The role of biological extracellular matrix scaffolds in vascularized three-dimensional tissue growthin vivo

2009 ◽  
Vol 89B (2) ◽  
pp. 575-575
Author(s):  
Kevin J. Cronin ◽  
Aurora Messina ◽  
Erik W. Thompson ◽  
Wayne A. Morrison ◽  
Geoffrey W. Stevens ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Three Dimensional ◽  
Extracellular Matrix Scaffolds

scholarly journals The role of biological extracellular matrix scaffolds in vascularized three-dimensional tissue growthin vivo

2008 ◽  
Vol 85B (1) ◽  
pp. 300-300
Author(s):  
Kevin J. Cronin ◽  
Aurora Messina ◽  
Erik W. Thompson ◽  
Wayne A. Morrison ◽  
Geoffrey W. Stevens ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Three Dimensional ◽  
Extracellular Matrix Scaffolds

Three-Dimensional Simulation of In Vitro Angiogenesis: Effects of Extracellular Matrix Structure and Density

2010 ◽  
Author(s):  
Lowell Taylor Edgar ◽  
James E. Guilkey ◽  
Clayton J. Underwood ◽  
Brenda Baggett ◽  
Urs Utzinger ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Endothelial Cells ◽  
Three Dimensional ◽  
Vascular Endothelial ◽  
Chemical Factors ◽  
Dimensional Simulation ◽  
Endothelial Growth Factor ◽  
In Vitro Angiogenesis

The process of angiogenesis is regulated by both chemical and mechanical signaling. While the role of chemical factors such as vascular endothelial growth factor (VEGF) during angiogenesis has been extensively studied, the influence of the mechanostructural environment on new vessel generation has received significantly less attention. During angiogenesis, endothelial cells in the existing vasculature detach and migrate out into the surrounding extracellular matrix (ECM), forming tubular structures that eventually mature into new blood vessels. This process is modulated by the structure and composition of the ECM [1]. The ECM is then remodeled by endothelial cells in the elongating neovessel tip, resulting in matrix condensation and changes in fiber orientation [2]. The mechanism as to how angiogenic vasculature and the ECM influence each other is poorly understood.

scholarly journals Three-Dimensional Extracellular Matrix Scaffolds by Microfluidic Fabrication for Long-Term Spontaneously Contracted Cardiomyocyte Culture

2014 ◽  
Vol 20 (21-22) ◽  
pp. 2931-2941 ◽  
Author(s):  
Jeng-Chun Mei ◽  
Aden Yuan Kun Wu ◽  
Po-Chen Wu ◽  
Nai-Chen Cheng ◽  
Wei-Bor Tsai ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Three Dimensional ◽  
Cardiomyocyte Culture ◽  
Extracellular Matrix Scaffolds

scholarly journals Three-dimensional migration of macrophages requires Hck for podosome organization and extracellular matrix proteolysis

Blood ◽  
2010 ◽  
Vol 115 (7) ◽  
pp. 1444-1452 ◽  
Author(s):  
Céline Cougoule ◽  
Véronique Le Cabec ◽  
Renaud Poincloux ◽  
Talal Al Saati ◽  
Jean-Louis Mège ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Spatial Organization ◽  
Ectopic Expression ◽  
Three Dimensional ◽  
Matrix Metalloproteases ◽  
3 Dimensional ◽  
Normal Expression ◽  
Migration Of Macrophages

Abstract Tissue infiltration of phagocytes exacerbates several human pathologies including chronic inflammations or cancers. However, the mechanisms involved in macrophage migration through interstitial tissues are poorly understood. We investigated the role of Hck, a Src-family kinase involved in the organization of matrix adhesion and degradation structures called podosomes. In Hck−/− mice submitted to peritonitis, we found that macrophages accumulated in interstitial tissues and barely reached the peritoneal cavity. In vitro, 3-dimensional (3D) migration and matrix degradation abilities, 2 protease-dependent properties of bone marrow–derived macrophages (BMDMs), were affected in Hck−/− BMDMs. These macrophages formed few and undersized podosome rosettes and, consequently, had reduced matrix proteolysis operating underneath despite normal expression and activity of matrix metalloproteases. Finally, in fibroblasts unable to infiltrate matrix, ectopic expression of Hck provided the gain–of–3D migration function, which correlated positively with formation of podosome rosettes. In conclusion, spatial organization of podosomes as large rosettes, proteolytic degradation of extracellular matrix, and 3D migration appeared to be functionally linked and regulated by Hck in macrophages. Hck, as the first protein combining a phagocyte-limited expression with a role in 3D migration, could be a target for new anti-inflammatory and antitumor molecules.

Three-dimensional reconstituted extracellular matrix scaffolds for tissue engineering

Biomaterials ◽  
2009 ◽  
Vol 30 (26) ◽  
pp. 4309-4317 ◽  
Author(s):  
Karthikeyan Narayanan ◽  
Kwong-Joo Leck ◽  
Shujun Gao ◽  
Andrew C.A. Wan
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Three Dimensional ◽  
Extracellular Matrix Scaffolds
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