The growth improvement of porcine esophageal smooth muscle cells on collagen-grafted poly(DL-lactide-co-glycolide) membrane

2005 ◽  
Vol 75B (1) ◽  
pp. 193-199 ◽  
Author(s):  
Yabin Zhu ◽  
Mary B. Chan-Park ◽  
Kerm Sin Chian
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Esophageal Smooth Muscle Cells

Human esophageal smooth muscle cells express muscarinic receptor subtypes M1 through M5

2000 ◽  
Vol 279 (5) ◽  
pp. G1059-G1069 ◽  
Author(s):  
Jian Wang ◽  
Pawel S. Krysiak ◽  
Lisanne G. Laurier ◽  
Stephen M. Sims ◽  
Harold G. Preiksaitis
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscarinic Receptor ◽  
Muscle Cells ◽  
Receptor Subtypes ◽  
Muscarinic Receptor Subtypes ◽  
Functional Responses ◽  
Selective Antagonist ◽  
Esophageal Physiology ◽  
Esophageal Smooth Muscle Cells

Receptor characterization in human esophageal smooth muscle is limited by tissue availability. We used human esophageal smooth muscle cells in culture to examine the expression and function of muscarinic receptors. Primary cultures were established using cells isolated by enzymatic digestion of longitudinal muscle (LM) and circular muscle (CM) obtained from patients undergoing esophagectomy for cancer. Cultured cells grew to confluence after 10–14 days in medium containing 10% fetal bovine serum and stained positively for anti-smooth muscle specific α-actin. mRNA encoding muscarinic receptor subtypes M1–M5 was identified by RT-PCR. The expression of corresponding protein for all five subtypes was confirmed by immunoblotting and immunocytochemistry. Functional responses were assessed by measuring free intracellular Ca2+ concentration ([Ca2+]i) using fura 2 fluorescence. Basal [Ca2+]i, which was 135 ± 22 nM, increased transiently to 543 ± 29 nM in response to 10 μM ACh in CM cells ( n = 8). This response was decreased <95% by 0.01 μM 4-diphenylacetoxy- N-methylpiperidine, a M1/M3-selective antagonist, whereas 0.1 μM methoctramine, a M2/M4-selective antagonist, and 0.1 μM pirenzepine, a M1-selective antagonist, had more modest effects. LM and CM cells showed similar results. We conclude that human smooth muscle cells in primary culture express five muscarinic receptor subtypes and respond to ACh with a rise in [Ca2+]i mediated primarily by the M3 receptor and involving release of Ca2+ from intracellular stores. This culture model provides a useful tool for further study of esophageal physiology.

The signaling of protease-activated receptor-2 activating peptide-induced contraction in cat esophageal smooth muscle cells

2017 ◽  
Vol 40 (12) ◽  
pp. 1443-1454 ◽  
Author(s):  
Hyun Su Ha ◽  
Se Eun Lee ◽  
Hyun Seok Lee ◽  
Gil Hyung Kim ◽  
Chan Jong Yoon ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Esophageal Smooth Muscle Cells ◽  
Protease Activated Receptor 2

Clonal overgrowth of esophageal smooth muscle cells in diffuse leiomyomatosis-Alport syndrome caused by partial deletion in COL4A5 and COL4A6 genes

2011 ◽  
Vol 30 (1) ◽  
pp. 3-8 ◽  
Author(s):  
Toshitaka Oohashi ◽  
Ichiro Naito ◽  
Yasuyoshi Ueki ◽  
Tomoki Yamatsuji ◽  
Rattiya Permpoon ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Alport Syndrome ◽  
Muscle Cells ◽  
Partial Deletion ◽  
Esophageal Smooth Muscle Cells

The signaling mechanism of the sphingosylphosphorylcholine-induced contraction in cat esophageal smooth muscle cells

2007 ◽  
Vol 30 (12) ◽  
pp. 1608-1618 ◽  
Author(s):  
Yong Sung Kim ◽  
Hyun Ju Song ◽  
Sun Young Park ◽  
Young Sil Min ◽  
Byung Ok Im ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Signaling Mechanism ◽  
Esophageal Smooth Muscle Cells

scholarly journals The Signaling Mechanism of Contraction Induced by ATP and UTP in Feline Esophageal Smooth Muscle Cells

2015 ◽  
Vol 38 (7) ◽  
pp. 616-623 ◽  
Author(s):  
Tae Hoon Kwon ◽  
Hyunwoo Jung ◽  
Eun Jeong Cho ◽  
Ji Hoon Jeong ◽  
Uy Dong Sohn
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Signaling Mechanism ◽  
Esophageal Smooth Muscle Cells

Mo1227 IN HUMAN ESOPHAGEAL SMOOTH MUSCLE CELLS, IL-4 STIMULATES EOTAXIN-3 SECRETION THAT IS BLOCKED ONLY MINIMALLY BY A PPI OR A P-CAB.

2020 ◽  
Vol 158 (6) ◽  
pp. S-834-S-835
Author(s):  
Melissa R. Nelson ◽  
Xi Zhang ◽  
Eitan Podgaetz ◽  
Vani J. Konda ◽  
Stuart J. Spechler ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Esophageal Smooth Muscle Cells
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